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Chelating calcium with BAPTA-AM or EGTA potently inhibited HDAC- and CMV-mediated MICA\/B expression. It was further observed that endoplasmic reticulum calcium stores were depleted after HDAC treatment. NF-\u03baB activity can be induced by HDAC treatment. However, nuclear translocation of NF-\u03baB p65 was not observed after HDAC treatment of Jurkat T cells and even though we could effectively inhibit p65 expression by siRNA, it did not modify MICA\/B expression. To identify important elements in MICA regulation, we made a promoter construct consisting of \u223c3 kb of the proximal MICA promoter in front of GFP. Deletion analysis showed that a germinal center-box containing a putative Sp1 site from position \u2212113 to \u221293 relative to the mRNA start site was important for HDAC and CMV-induced promoter activity. Sp1 was subsequently shown to be important, as targeted mutation of the Sp1 binding sequence or siRNA mediated down modulation of Sp1-inhibited MICA promoter activity and surface-expression.<\/jats:p>","DOI":"10.4049\/jimmunol.179.12.8235","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:25:48Z","timestamp":1397849148000},"page":"8235-8242","source":"Crossref","is-referenced-by-count":49,"title":["Molecular Regulation of MHC Class I Chain-Related Protein A Expression after HDAC-Inhibitor Treatment of Jurkat T Cells"],"prefix":"10.1093","volume":"179","author":[{"given":"Lars","family":"Andresen","sequence":"first","affiliation":[{"name":"Department of Immunology, Institute of International Health, Immunology and Microbiology, University of Copenhagen , Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helle","family":"Jensen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of International Health, Immunology and Microbiology, University of Copenhagen , Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marianne T","family":"Pedersen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of International Health, Immunology and Microbiology, University of Copenhagen , Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen A","family":"Hansen","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of International Health, Immunology and Microbiology, University of Copenhagen , Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S\u00f8ren","family":"Skov","sequence":"additional","affiliation":[{"name":"Department of Immunology, Institute of International Health, Immunology and Microbiology, University of Copenhagen , Copenhagen ,","place":["Denmark"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2007,12,15]]},"reference":[{"key":"2026012809223442400_R1","first-page":"727","volume-title":"Science","year":"1999"},{"key":"2026012809223442400_R2","first-page":"755","volume-title":"Nat. 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