{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T11:45:56Z","timestamp":1791287156575,"version":"4.1.0"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>It is well-established that heat shock proteins (HSPs)-peptides complexes elicit antitumor responses in prophylactic and therapeutic immunization protocols. HSPs such as gp96 and Hsp70 have been demonstrated to undergo receptor-mediated uptake by APCs with subsequent representation of the HSP-associated peptides to MHC class I molecules on APCs, facilitating efficient cross-presentation. On the contrary, despite its abundant expression among HSPs in the cytosol, the role of Hsp90 for the cross-presentation remains unknown. We show here that exogenous Hsp90-peptide complexes can gain access to the MHC class I presentation pathway and cause cross-presentation by bone marrow-derived dendritic cells. Interestingly, this presentation is TAP independent, and followed chloroquine, leupeptin-sensitive, as well as cathepsin S-dependent endosomal pathways. In addition, we show that Hsp90-chaperoned precursor peptides are processed and transferred onto MHC class I molecules in the endosomal compartment. Furthermore, we demonstrate that immunization with Hsp90-peptide complexes induce Ag-specific CD8+ T cell responses and strong antitumor immunity in vivo. These findings have significant implications for the design of T cell-based cancer immunotherapy.<\/jats:p>","DOI":"10.4049\/jimmunol.179.3.1803","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T22:56:47Z","timestamp":1397861807000},"page":"1803-1813","source":"Crossref","is-referenced-by-count":85,"title":["Efficient Cross-Presentation by Heat Shock Protein 90-Peptide Complex-Loaded Dendritic Cells via an Endosomal Pathway"],"prefix":"10.1093","volume":"179","author":[{"given":"Takehiro","family":"Kurotaki","sequence":"first","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]},{"name":"Department of Surgery, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuaki","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gosei","family":"Ueda","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]},{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of the Ryukyus , Okinawa ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Oura","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Goro","family":"Kutomi","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshihiko","family":"Hirohashi","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroeki","family":"Sahara","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Torigoe","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyoshi","family":"Hiratsuka","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of the Ryukyus , Okinawa ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hajime","family":"Sunakawa","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery, Faculty of Medicine, University of the Ryukyus , Okinawa ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koichi","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Surgery, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Noriyuki","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Pathology, School of Medicine, Sapporo Medical University , Sapporo ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,8,1]]},"reference":[{"key":"2025032701455031200_R1","first-page":"395","volume-title":"Annu. 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