{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:11:00Z","timestamp":1791047460154,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Whether IFN-\u03b3 contributes to the per-cell protective capacity of memory CD8+ T cells against Listeria monocytogenes (LM) has not been formally tested. In this study, we generated LM Ag-specific memory CD8+ T cells via immunization of wild-type (WT) and IFN-\u03b3-deficient (gamma knockout (GKO)) mice with LM peptide-coated dendritic cells and compared them phenotypically and functionally. Immunization of WT and GKO mice resulted in memory CD8+ T cells that were similar in number, functional avidity, TCR repertoire use, and memory phenotype. The protective capacity of memory CD8+ T cells from immunized WT and GKO mice was evaluated after adoptive transfer of equal numbers of WT or GKO cells into naive BALB\/c mice followed by LM challenge. The adoptively transferred CD8+ T cells from GKO donors exhibited a decreased ability to reduce bacterial numbers in the organs of recipient mice when compared with an equivalent number of Ag-matched WT CD8+ T cells. This deficiency was most evident early (day 3) after infection if a relatively low infectious dose was used; however, transferring fewer memory CD8+ T cells or increasing the LM challenge dose revealed a more pronounced defect in protective immunity mediated by the CD8+ T cells from GKO mice. Our studies identified a decrease in Ag-specific target cell lysis in vivo by CD8+ T cells from GKO mice as the mechanism for the decreased protective immunity after LM challenge. Further studies suggest that the lack of IFN-\u03b3 production by the Ag-specific CD8 T cells themselves diminishes target cell sensitivity to cytolysis, thereby reducing the lytic potency of IFN-\u03b3-deficient LM-specific memory CD8+ T cells.<\/jats:p>","DOI":"10.4049\/jimmunol.179.4.2457","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T18:56:09Z","timestamp":1397847369000},"page":"2457-2466","source":"Crossref","is-referenced-by-count":32,"title":["A Role for IFN-\u03b3 from Antigen-Specific CD8+ T Cells in Protective Immunity to\n                    <i>Listeria monocytogenes<\/i>"],"prefix":"10.1093","volume":"179","author":[{"given":"Kelly A N","family":"Messingham","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir P","family":"Badovinac","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali","family":"Jabbari","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John T","family":"Harty","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Iowa , Iowa City, IA 52242"},{"name":"Interdisciplinary Program in Immunology, University of Iowa , Iowa City, IA 52242"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,8,15]]},"reference":[{"key":"2026012809183992200_R1","first-page":"360","volume-title":"Curr. 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