{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:33:15Z","timestamp":1790969595773,"version":"4.1.0"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Binding of peptide\/MHC (pMHC) complexes by TCR initiates T cell activation. Despite long interest, the exact relationship between the biochemistry of TCR\/pMHC interaction (particularly TCR affinity or ligand off-rate) and T cell responses remains unresolved, because the number of complexes examined in each independent system has been too small to draw a definitive conclusion. To test the current models of T cell activation, we have analyzed the interactions between the mouse P14 TCR and a set of altered peptides based on the lymphocytic choriomeningitis virus epitope gp33\u201341 sequence bound to mouse class I MHC Db. pMHC binding, TCR-binding characteristics, CD8+ T cell cytotoxicity, and IFN-\u03b3 production were measured for the peptides. We found affinity correlated well with both cytotoxicity and IFN-\u03b3 production. In contrast, no correlation was observed between any kinetic parameter of TCR-pMHC interaction and cytotoxicity or IFN-\u03b3 production. This study strongly argues for an affinity threshold model of T cell activation.<\/jats:p>","DOI":"10.4049\/jimmunol.179.5.2952","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:17:46Z","timestamp":1397848666000},"page":"2952-2960","source":"Crossref","is-referenced-by-count":124,"title":["CD8+ T Cell Activation Is Governed by TCR-Peptide\/MHC Affinity, Not Dissociation Rate"],"prefix":"10.1093","volume":"179","author":[{"given":"Shaomin","family":"Tian","sequence":"first","affiliation":[{"name":"Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Maile","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"},{"name":"Surgery, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward J","family":"Collins","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"},{"name":"Biochemistry and Biophysics, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey A","family":"Frelinger","sequence":"additional","affiliation":[{"name":"Microbiology and Immunology, University of North Carolina , Chapel Hill, NC 27599"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,9,1]]},"reference":[{"key":"2026012809341132400_R1","first-page":"523","volume-title":"Annu. 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