{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T01:19:43Z","timestamp":1791595183068,"version":"4.3.4"},"reference-count":31,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2007,9,1]],"date-time":"2007-09-01T00:00:00Z","timestamp":1188604800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2007,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A soluble recombinant form of Neisseria meningitidis adhesin A (NadA\u0394351\u2013405), proposed as a constituent of anti-meningococcal B vaccines, is here shown to specifically interact with and immune-modulate human monocyte-derived dendritic cells (mo-DCs). After priming with IFN-\u03b3 and stimulation with NadA\u0394351\u2013405, mo-DCs strongly up-regulated maturation markers CD83, CD86, CD80, and HLA-DR, secreted moderate quantities of TNF-\u03b1, IL-6, and IL-8, and produced a slight, although significant, amount of IL-12p70. Costimulation of mo-DCs with NadA\u0394351\u2013405 and the imidoazoquinoline drug R-848, believed to mimic bacterial RNA, increased CD86 in an additive way, but strongly synergized the secretion of IL-12p70, IL-1, IL-6, TNF-\u03b1, and MIP-1\u03b1, especially after IFN-\u03b3 priming. CD86\/CD80 overexpression correlated with the occupation of high-(kd \u223c 80 nM) and low-(kd \u223c 4 \u03bcM) affinity binding sites for NadA\u0394351\u2013405. Alternatively, secretion of IL-12p70 and TNF-\u03b1, IL-6, and IL-8 corresponded to the occupation of high- or low-affinity receptors, respectively. Mo-DCs matured by IFN-\u03b3 and NadA\u0394351\u2013405 supported the proliferation of naive CD4+ T lymphocytes, inducing the differentiation of both IFN-\u03b3 and IL-4 producing phenotypes. Our data show that NadA not only is a good immunogen but is as well endowed with a proimmune, self-adjuvating, activity.<\/jats:p>","DOI":"10.4049\/jimmunol.179.6.3904","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:32:36Z","timestamp":1397849556000},"page":"3904-3916","source":"Crossref","is-referenced-by-count":23,"title":["IFN-\u03b3 and R-848 Dependent Activation of Human Monocyte-Derived Dendritic Cells by\n                    <i>Neisseria meningitidis<\/i>\n                    Adhesin A"],"prefix":"10.1093","volume":"179","author":[{"given":"Cristina","family":"Mazzon","sequence":"first","affiliation":[{"name":"Centro Ricerche Interdipartimentale Biotecnologie Innovative and Dipartimento di Scienze Biomediche Sperimentali, Universita\u0300 di Padova , Padova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Baldani-Guerra","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia, Sezione di Immunologia, Universita\u0300 di Verona , Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Cecchini","sequence":"additional","affiliation":[{"name":"Centro Ricerche Interdipartimentale Biotecnologie Innovative and Dipartimento di Scienze Biomediche Sperimentali, Universita\u0300 di Padova , Padova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tihana","family":"Kasic","sequence":"additional","affiliation":[{"name":"Istituto Veneto Medicina Molecolare and Dipartimento di Scienze Biomediche Sperimentali, Universita\u0300 di Padova , Padova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Viola","sequence":"additional","affiliation":[{"name":"Istituto Veneto Medicina Molecolare and Dipartimento di Scienze Biomediche Sperimentali, Universita\u0300 di Padova , Padova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"de Bernard","sequence":"additional","affiliation":[{"name":"Istituto Veneto Medicina Molecolare and Dipartimento di Biologia, Universita\u0300 di Padova , Padova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beatrice","family":"Arico\u0300","sequence":"additional","affiliation":[{"name":"Research Centre, Novartis Vaccines and Diagnostics , Siena ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franca","family":"Gerosa","sequence":"additional","affiliation":[{"name":"Dipartimento di Patologia, Sezione di Immunologia, Universita\u0300 di Verona , Verona ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuele","family":"Papini","sequence":"additional","affiliation":[{"name":"Centro Ricerche Interdipartimentale Biotecnologie Innovative and Dipartimento di Scienze Biomediche Sperimentali, Universita\u0300 di Padova , Padova ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2007,9,15]]},"reference":[{"key":"2026012809262546600_R1","first-page":"144","volume-title":"Clin. Microbiol. Rev.","year":"2000"},{"key":"2026012809262546600_R2","first-page":"967","volume-title":"Pediatr. Infect. Dis. J.","year":"1996"},{"key":"2026012809262546600_R3","first-page":"1816","volume-title":"Science","year":"2000"},{"key":"2026012809262546600_R4","first-page":"787","volume-title":"Nature","year":"1996"},{"key":"2026012809262546600_R5","first-page":"251","volume-title":"Nat. Rev. Immunol.","year":"2005"},{"key":"2026012809262546600_R6","first-page":"289","volume-title":"Springer Semin. Immunopathol.","year":"2005"},{"key":"2026012809262546600_R7","first-page":"1551","volume-title":"J. Immunol.","year":"2005"},{"key":"2026012809262546600_R8","first-page":"1636","volume-title":"J. Immunol.","year":"2005"},{"key":"2026012809262546600_R9","first-page":"147","volume-title":"J. Exp. Med.","year":"1996"},{"key":"2026012809262546600_R10","first-page":"1593","volume-title":"Int. Immunol.","year":"1998"},{"key":"2026012809262546600_R11","first-page":"4351","volume-title":"Infect. Immun.","year":"2001"},{"key":"2026012809262546600_R12","first-page":"5590","volume-title":"Infect. Immun.","year":"2004"},{"key":"2026012809262546600_R13","first-page":"651","volume-title":"Cell. Microbiol.","year":"2004"},{"key":"2026012809262546600_R14","first-page":"1809","volume-title":"Science","year":"2000"},{"key":"2026012809262546600_R15","first-page":"1445","volume-title":"J. Exp. Med.","year":"2002"},{"key":"2026012809262546600_R16","first-page":"5989","volume-title":"EMBO J.","year":"2000"},{"key":"2026012809262546600_R17","first-page":"4217","volume-title":"Infect. Immun.","year":"2004"},{"key":"2026012809262546600_R18","first-page":"687","volume-title":"Mol. Microbiol.","year":"2005"},{"key":"2026012809262546600_R19","first-page":"10834","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2006"},{"key":"2026012809262546600_R20","first-page":"1983","volume-title":"J. Immunol.","year":"2005"},{"key":"2026012809262546600_R21","first-page":"36560","volume-title":"J. Biol. Chem.","year":"2006"},{"key":"2026012809262546600_R22","first-page":"1109","volume-title":"J. Exp. Med.","year":"1994"},{"key":"2026012809262546600_R23","first-page":"1","volume-title":"Genome Biol.","year":"2002"},{"key":"2026012809262546600_R24","first-page":"641","volume-title":"Immunity","year":"2003"},{"key":"2026012809262546600_R25","first-page":"769","volume-title":"Nat. Immunol.","year":"2005"},{"key":"2026012809262546600_R26","first-page":"196","volume-title":"Nat. Immunol.","year":"2002"},{"key":"2026012809262546600_R27","first-page":"499","volume-title":"Nat. Immunol.","year":"2002"},{"key":"2026012809262546600_R28","first-page":"933","volume-title":"Int. Immunol.","year":"2001"},{"key":"2026012809262546600_R29","first-page":"415","volume-title":"Eur. J. Immunol.","year":"2000"},{"key":"2026012809262546600_R30","first-page":"2543","volume-title":"Eur. J. Immunol.","year":"2002"},{"key":"2026012809262546600_R31","first-page":"2987","volume-title":"Eur. J. Immunol.","year":"2003"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/179\/6\/3904\/62666009\/zim01807003904.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/179\/6\/3904\/62666009\/zim01807003904.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T14:26:35Z","timestamp":1769610395000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/179\/6\/3904\/8022087"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,9]]},"references-count":31,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2007,9,15]]},"published-print":{"date-parts":[[2007,9,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.179.6.3904","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2007,9]]},"published":{"date-parts":[[2007,9]]}}}