{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:59:24Z","timestamp":1791255564349,"version":"4.1.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Listeria monocytogenes escapes from the phagosome of macrophages and replicates within the cytosolic compartment. The macrophage responds to L. monocytogenes through detection pathways located on the cell surface (TLRs) and within the cytosol (Nod-like receptors) to promote inflammatory processes aimed at clearing the pathogen. Cytosolic L. monocytogenes activates caspase 1, resulting in post-translational processing of the cytokines IL-1\u03b2 and IL-18 as well as caspase 1-dependent cell death (pyroptosis). We demonstrate that the presence of L. monocytogenes within the cytosolic compartment induces caspase 1 activation through multiple Nod-like receptors, including Ipaf and Nalp3. Flagellin expression by cytosolic L. monocytogenes was detected through Ipaf in a dose-dependent manner. Concordantly, detection of flagellin promoted bacterial clearance in a murine infection model. Finally, we provide evidence that suggests cytosolic L. monocytogenes activates caspase 1 through a third pathway, which signals through the adaptor protein ASC. Thus, L. monocytogenes activates caspase 1 in macrophages via multiple pathways, all of which detect the presence of bacteria within the cytosol.<\/jats:p>","DOI":"10.4049\/jimmunol.180.11.7558","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:09:57Z","timestamp":1397848197000},"page":"7558-7564","source":"Crossref","is-referenced-by-count":152,"title":["Multiple Nod-Like Receptors Activate Caspase 1 during\n                    <i>Listeria monocytogenes<\/i>\n                    Infection"],"prefix":"10.1093","volume":"180","author":[{"given":"Sarah E","family":"Warren","sequence":"first","affiliation":[{"name":"Department of Immunology, University of Washington , Seattle WA 98195"},{"name":"Institute for Systems Biology , Seattle WA 98103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dat P","family":"Mao","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology , Seattle WA 98103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April E","family":"Rodriguez","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology , Seattle WA 98103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward A","family":"Miao","sequence":"additional","affiliation":[{"name":"Institute for Systems Biology , Seattle WA 98103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan","family":"Aderem","sequence":"additional","affiliation":[{"name":"Department of Immunology, University of Washington , Seattle WA 98195"},{"name":"Institute for Systems Biology , Seattle WA 98103"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,6,1]]},"reference":[{"key":"2026012809144752300_R1","first-page":"816","volume-title":"Cell Death Differ.","year":"2006"},{"key":"2026012809144752300_R2","first-page":"1","volume-title":"Cell. Microbiol.","year":"2008"},{"key":"2026012809144752300_R3","first-page":"214","volume-title":"Immunol. Rev.","year":"2007"},{"key":"2026012809144752300_R4","first-page":"1167","volume-title":"Microbes Infect.","year":"2007"},{"key":"2026012809144752300_R5","first-page":"1176","volume-title":"Microbes Infect.","year":"2007"},{"key":"2026012809144752300_R6","first-page":"272","volume-title":"Trends Microbiol.","year":"1997"},{"key":"2026012809144752300_R7","first-page":"215","volume-title":"J. Pathol.","year":"2006"},{"key":"2026012809144752300_R8","first-page":"4411","volume-title":"J. Immunol.","year":"2007"},{"key":"2026012809144752300_R9","first-page":"147","volume-title":"J. Mol. Med.","year":"2002"},{"key":"2026012809144752300_R10","first-page":"45","volume-title":"Curr. Opin. Microbiol.","year":"2004"},{"key":"2026012809144752300_R11","first-page":"499","volume-title":"Nat. Rev. Immunol.","year":"2004"},{"key":"2026012809144752300_R12","first-page":"13861","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2002"},{"key":"2026012809144752300_R13","first-page":"19","volume-title":"Ann. NY Acad. Sci.","year":"2006"},{"key":"2026012809144752300_R14","first-page":"12","volume-title":"PLoS Pathog.","year":"2008"},{"key":"2026012809144752300_R15","first-page":"228","volume-title":"Nature","year":"2006"},{"key":"2026012809144752300_R16","first-page":"790","volume-title":"J. Exp. Med.","year":"1985"},{"key":"2026012809144752300_R17","first-page":"213","volume-title":"J. Leukocyte Biol.","year":"2003"},{"key":"2026012809144752300_R18","first-page":"561","volume-title":"Cell","year":"2004"},{"key":"2026012809144752300_R19","first-page":"615","volume-title":"Curr. Opin. Immunol.","year":"2007"},{"key":"2026012809144752300_R20","first-page":"233","volume-title":"Nature","year":"2006"},{"key":"2026012809144752300_R21","first-page":"1583","volume-title":"Cell Death Differ.","year":"2007"},{"key":"2026012809144752300_R22","first-page":"569","volume-title":"Nat. Immunol.","year":"2006"},{"key":"2026012809144752300_R23","first-page":"576","volume-title":"Nat. Immunol.","year":"2006"},{"key":"2026012809144752300_R24","first-page":"33897","volume-title":"J. Biol. Chem.","year":"2007"},{"key":"2026012809144752300_R25","first-page":"35217","volume-title":"J. Biol. Chem.","year":"2006"},{"key":"2026012809144752300_R26","first-page":"1093","volume-title":"J. Exp. Med.","year":"2006"},{"key":"2026012809144752300_R27","first-page":"e18","volume-title":"PLoS Pathog.","year":"2006"},{"key":"2026012809144752300_R28","first-page":"275","volume-title":"Semin. Immunopathol.","year":"2007"},{"key":"2026012809144752300_R29","first-page":"e111","volume-title":"PLoS Pathog.","year":"2007"},{"key":"2026012809144752300_R30","first-page":"18810","volume-title":"J. Biol. Chem.","year":"2007"},{"key":"2026012809144752300_R31","first-page":"335","volume-title":"Int. Immunol.","year":"2004"},{"key":"2026012809144752300_R32","first-page":"235","volume-title":"Cell. Microbiol.","year":"2004"},{"key":"2026012809144752300_R33","first-page":"1065","volume-title":"Int. Immunol.","year":"2002"},{"key":"2026012809144752300_R34","first-page":"5165","volume-title":"J. Immunol.","year":"2003"},{"key":"2026012809144752300_R35","first-page":"664","volume-title":"Microbes Infect.","year":"2007"},{"key":"2026012809144752300_R36","first-page":"213","volume-title":"Nature","year":"2004"},{"key":"2026012809144752300_R37","first-page":"259","volume-title":"J. Leukocyte Biol.","year":"2007"},{"key":"2026012809144752300_R38","first-page":"417","volume-title":"Mol. Cell.","year":"2002"},{"key":"2026012809144752300_R39","first-page":"319","volume-title":"Immunity","year":"2004"},{"key":"2026012809144752300_R40","first-page":"15195","volume-title":"J. Biol. Chem.","year":"1994"},{"key":"2026012809144752300_R41","first-page":"184","volume-title":"J. Biochem.","year":"1988"},{"key":"2026012809144752300_R42","first-page":"2344","volume-title":"Cell. Microbiol.","year":"2007"},{"key":"2026012809144752300_R43","first-page":"3030","volume-title":"Eur. J. Immunol.","year":"2007"},{"key":"2026012809144752300_R44","first-page":"3235","volume-title":"J. Exp. Med.","year":"2007"},{"key":"2026012809144752300_R45","first-page":"2562","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2008"},{"key":"2026012809144752300_R46","first-page":"213","volume-title":"Semin. Immunopathol.","year":"2007"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/180\/11\/7558\/62724685\/zim01108007558.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/180\/11\/7558\/62724685\/zim01108007558.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T14:14:54Z","timestamp":1769609694000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/180\/11\/7558\/8021229"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6,1]]},"references-count":46,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2008,6,1]]},"published-print":{"date-parts":[[2008,6,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.180.11.7558","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2008,6]]},"published":{"date-parts":[[2008,6,1]]}}}