{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T03:02:24Z","timestamp":1769655744928,"version":"3.49.0"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,3,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The intracytosolic niche for replication of Listeria monocytogenes (Lm) facilitates delivery of bacteria-derived Ags into the MHC class I pathway for subsequent stimulation of CD8 effector T cells. Using Lm strains that are equivalent for in vivo virulence yet express marked differences in the level of secretion of a protective target Ag, we have evaluated how these specific differences in secretion levels influences the magnitude and effector function of Ag-specific CD8 T cell responses following Lm injection. Immunization with low doses of a hyperantigen-secreting Lm strain stimulated enhanced target-Ag specific CD8 T cell responses compared with the magnitude stimulated following immunization with the same dose of wild-type Lm. The enhanced determinant-specific response was also evident by in vivo CTL activity, increased numbers of memory cells 4 wk following immunization, and enhanced antilisterial protection following a challenge infection. Initiation of antibiotic treatment 24 h following infection with wild-type Lm markedly reduced the magnitude of the effector CD8 T cell response. In contrast, antibiotic treatment initiated 24 h following immunization with the hyperantigen secreting strain of Lm did not impact the frequency of the target-Ag specific CD8 T cells. Thus, immunization with a low dose of a hyperantigen secreting Lm strain, followed by antibiotic treatment to limit the extent of the infection, may represent a safe strategy for the stimulation of enhanced effector CD8 T cell responses to specific Ag by a rLm vaccine.<\/jats:p>","DOI":"10.4049\/jimmunol.180.5.3406","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:16:52Z","timestamp":1397848612000},"page":"3406-3416","source":"Crossref","is-referenced-by-count":7,"title":["Stimulation of Enhanced CD8 T Cell Responses Following Immunization with a Hyper-Antigen Secreting Intracytosolic Bacterial Pathogen"],"prefix":"10.1093","volume":"180","author":[{"given":"Megan J","family":"Smithey","sequence":"first","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Brandt","sequence":"additional","affiliation":[{"name":"Earle A. Chiles Research Institute, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy E","family":"Freitag","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois , Chicago, IL 60612"},{"name":"Seattle Biomedical Research Institute, University of Washington , Seattle, WA 98195"},{"name":"Departments of Pathobiology and Microbiology, University of Washington , Seattle, WA 98195"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Darren E","family":"Higgins","sequence":"additional","affiliation":[{"name":"Department of Molecular Genetics and Microbiology, Harvard Medical School , Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H G Archie","family":"Bouwer","sequence":"additional","affiliation":[{"name":"Veterans Affairs Medical Center, Oregon Health and Science University , Portland, OR 97239"},{"name":"Earle A. Chiles Research Institute, Oregon Health and Science University , Portland, OR 97239"},{"name":"Department of Molecular Microbiology and Immunology, Oregon Health and Science University , Portland, OR 97239"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,3,1]]},"reference":[{"key":"2026012809410180100_R1","first-page":"3987","volume-title":"Proc. Natl. Acad. Sci. USA","year":"1995"},{"key":"2026012809410180100_R2","first-page":"5264","volume-title":"Cancer Res.","year":"1999"},{"key":"2026012809410180100_R3","first-page":"346","volume-title":"Clin. Immunol.","year":"2001"},{"key":"2026012809410180100_R4","first-page":"454","volume-title":"Curr. Opin. Mol. Ther.","year":"2005"},{"key":"2026012809410180100_R5","first-page":"420","volume-title":"J. Immunol.","year":"2004"},{"key":"2026012809410180100_R6","first-page":"853","volume-title":"Nat. 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