{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:41:14Z","timestamp":1790613674158,"version":"4.1.0"},"reference-count":44,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:00:00Z","timestamp":1207008000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>1\u03b1,25-Dihydroxyvitamin D3 (1,25(OH)2D3), a potent inhibitor of NF-\u03baB expression, can prevent the maturation of dendritic cells in vitro leading to tolerogenic dendritic cells with increased potential to induce regulatory T cells. Herein, we investigated whether the combination of allergen immunotherapy with 1,25(OH)2D3 potentiates the suppressive effects of immunotherapy and whether the immunoregulatory cytokines IL-10 and TGF-\u03b2 are involved in the effector phase. OVA-sensitized and challenged BALB\/c mice displayed airway hyperresponsiveness (AHR) and increased serum OVA-specific IgE levels, bronchoalveolar lavage eosinophilia, and Th2 cytokine levels. In this model, the dose response of allergen immunotherapy 10 days before OVA inhalation challenge shows strong suppression of asthma manifestations at 1 mg of OVA, but partial suppression of bronchoalveolar lavage eosinophilia, IgE up-regulation, and no reduction of AHR at 100 \u03bcg. Interestingly, coadministration of 10 ng of 1,25(OH)2D3 with 100 \u03bcg of OVA immunotherapy significantly inhibited AHR and potentiated the reduction of serum OVA-specific IgE levels, airway eosinophilia, and Th2-related cytokines concomitant with increased IL-10 levels in lung tissues and TGF-\u03b2 and OVA-specific IgA levels in serum. Similar effects on suboptimal immunotherapy were observed by inhibition of the NF-\u03baB pathway using the selective I\u03baB kinase 2 inhibitor PS-1145. The suppressive effects of this combined immunotherapy were partially reversed by treatment with mAb to either IL-10R or TGF-\u03b2 before OVA inhalation challenge but completely abrogated when both Abs were given. These data demonstrate that 1,25(OH)2D3 potentiates the efficacy of immunotherapy and that the regulatory cytokines IL-10 and TGF-\u03b2 play a crucial role in the effector phase of this mouse model.<\/jats:p>","DOI":"10.4049\/jimmunol.180.8.5211","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T18:52:42Z","timestamp":1397847162000},"page":"5211-5221","source":"Crossref","is-referenced-by-count":136,"title":["1\u03b1,25-Dihydroxyvitamin D3 Potentiates the Beneficial Effects of Allergen Immunotherapy in a Mouse Model of Allergic Asthma: Role for IL-10 and TGF-\u03b2"],"prefix":"10.1093","volume":"180","author":[{"given":"Yousef A","family":"Taher","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University , Utrecht ,","place":["The Netherlands"]},{"name":"Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, Groningen University , Groningen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Betty C A M","family":"van Esch","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University , Utrecht ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerard A","family":"Hofman","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University , Utrecht ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul A J","family":"Henricks","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University , Utrecht ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoon J M","family":"van Oosterhout","sequence":"additional","affiliation":[{"name":"Laboratory of Allergology and Pulmonary Diseases, University Medical Center Groningen, Groningen University , Groningen ,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,4,15]]},"reference":[{"key":"2026012809161250800_R1","first-page":"558","volume-title":"J. 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