{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:27:58Z","timestamp":1787030878099,"version":"build-2736575974"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Perforin-2, the product of the MPEG1 gene, limits the spread and dissemination of bacterial pathogens in vivo. It is highly expressed in murine and human phagocytes, and macrophages lacking Perforin-2 are compromised in their ability to kill phagocytosed bacteria. In this study, we used Salmonella enterica serovar Typhimurium as a model intracellular pathogen to elucidate the mechanism of Perforin-2\u2019s bactericidal activity. In vitro Perforin-2 was found to facilitate the degradation of Ags contained within the envelope of phagocytosed bacteria. In contrast, degradation of a representative surface Ag was found to be independent of Perforin-2. Consistent with our in vitro results, a protease-sensitive, periplasmic superoxide dismutase (SodCII) contributed to the virulence of S. Typhimurium in Perforin-2 knockout but not wild-type mice. In aggregate, our studies indicate that Perforin-2 breaches the envelope of phagocytosed bacteria, facilitating the delivery of proteases and other antimicrobial effectors to sites within the bacterial cell.<\/jats:p>","DOI":"10.4049\/jimmunol.1800365","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T11:05:21Z","timestamp":1537787121000},"page":"2710-2720","source":"Crossref","is-referenced-by-count":24,"title":["Perforin-2 Breaches the Envelope of Phagocytosed Bacteria Allowing Antimicrobial Effectors Access to Intracellular Targets"],"prefix":"10.1093","volume":"201","author":[{"given":"Fangfang","family":"Bai","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, University of Miami Miller School of Medicine , Miami, FL 33136"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan M","family":"McCormack","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Miami Miller School of Medicine , Miami, FL 33136"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7432-3046","authenticated-orcid":false,"given":"Suzanne","family":"Hower","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Miami Miller School of Medicine , Miami, FL 33136"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory V","family":"Plano","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Miami Miller School of Medicine , Miami, FL 33136"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mathias G","family":"Lichtenheld","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Miami Miller School of Medicine , Miami, FL 33136"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3692-8199","authenticated-orcid":false,"given":"George P","family":"Munson","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Miami Miller School of Medicine , Miami, FL 33136"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,11,1]]},"reference":[{"key":"2025030417574033800_r1","doi-asserted-by":"crossref","first-page":"3277","DOI":"10.1016\/j.bbagen.2014.07.022","article-title":"Assembly of phagocyte NADPH oxidase: a concerted binding process?","volume":"1840","author":"Karimi","year":"2014","journal-title":"Biochim. 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