{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,2]],"date-time":"2026-11-02T17:06:46Z","timestamp":1793639206695,"version":"4.5.1"},"reference-count":94,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2025,2,26]],"date-time":"2025-02-26T00:00:00Z","timestamp":1740528000000},"content-version":"vor","delay-in-days":2279,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In transplantation, development of humoral alloimmunity against donor HLA is a major cause of organ transplant failure, but our ability to assess the immunological risk associated with a potential donor\u2013recipient HLA combination is limited. We hypothesized that the capacity of donor HLA to induce a specific alloantibody response depends on their structural and physicochemical dissimilarity compared with recipient HLA. To test this hypothesis, we first developed a novel computational scoring system that enables quantitative assessment of surface electrostatic potential differences between donor and recipient HLA molecules at the tertiary structure level [three-dimensional electrostatic mismatch score (EMS-3D)]. We then examined humoral alloimmune responses in healthy females subjected to a standardized injection of donor lymphocytes from their male partner. This analysis showed a strong association between the EMS-3D of donor HLA and donor-specific alloantibody development; this relationship was strongest for HLA-DQ alloantigens. In the clinical transplantation setting, the immunogenic potential of HLA-DRB1 and -DQ mismatches expressed on donor kidneys, as assessed by their EMS-3D, was an independent predictor of development of donor-specific alloantibody after graft failure. Collectively, these findings demonstrate the translational potential of our approach to improve immunological risk assessment and to decrease the burden of humoral alloimmunity in organ transplantation.<\/jats:p>","DOI":"10.4049\/jimmunol.1800683","type":"journal-article","created":{"date-parts":[[2018,11,14]],"date-time":"2018-11-14T11:25:44Z","timestamp":1542194744000},"page":"3780-3792","source":"Crossref","is-referenced-by-count":70,"title":["Predicting Humoral Alloimmunity from Differences in Donor and Recipient HLA Surface Electrostatic Potential"],"prefix":"10.1093","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7434-1201","authenticated-orcid":false,"given":"Dermot H","family":"Mallon","sequence":"first","affiliation":[{"name":"Department of Surgery, University of Cambridge, Addenbrooke\u2019s Hospital , Cambridge CB2 0QQ ,","place":["United Kingdom"]},{"name":"National Institute for Health Research Blood and Transplant Research Unit in Organ Donation and Transplantation, University of Cambridge , Cambridge CB2 0QQ ,","place":["United Kingdom"]},{"name":"National Institute of Health Research Cambridge Biomedical Research Centre , Cambridge CB2 0QQ ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8809-9708","authenticated-orcid":false,"given":"Christiane","family":"Kling","sequence":"additional","affiliation":[{"name":"Institute for Immunology, University Medical Centre Schleswig-Holstein, Kiel University , 24105 Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Robb","sequence":"additional","affiliation":[{"name":"Statistics and Clinical Studies Unit, National Health Service Blood and Transplant , Bristol BS34 7QH ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2914-3382","authenticated-orcid":false,"given":"Eva","family":"Ellinghaus","sequence":"additional","affiliation":[{"name":"Institute of Clinical Molecular Biology, University Medical Centre Schleswig-Holstein, Kiel University , 24105 Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J Andrew","family":"Bradley","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Cambridge, Addenbrooke\u2019s Hospital , Cambridge CB2 0QQ ,","place":["United Kingdom"]},{"name":"National Institute for Health Research Blood and Transplant Research Unit in Organ Donation and Transplantation, University of Cambridge , Cambridge CB2 0QQ ,","place":["United Kingdom"]},{"name":"National Institute of Health Research Cambridge Biomedical Research Centre , Cambridge CB2 0QQ ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig J","family":"Taylor","sequence":"additional","affiliation":[{"name":"National Institute of Health Research Cambridge Biomedical Research Centre , Cambridge CB2 0QQ ,","place":["United Kingdom"]},{"name":"Tissue Typing Laboratory, Cambridge University Hospitals National Health Service Foundation Trust , Cambridge CB2 0QQ ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Kabelitz","sequence":"additional","affiliation":[{"name":"Institute for Immunology, University Medical Centre Schleswig-Holstein, Kiel University , 24105 Kiel ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7298-1387","authenticated-orcid":false,"given":"Vasilis","family":"Kosmoliaptsis","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Cambridge, Addenbrooke\u2019s Hospital , Cambridge CB2 0QQ ,","place":["United Kingdom"]},{"name":"National Institute for Health Research Blood and Transplant Research Unit in Organ Donation and Transplantation, University of Cambridge , Cambridge CB2 0QQ ,","place":["United Kingdom"]},{"name":"National Institute of Health Research Cambridge Biomedical Research Centre , Cambridge CB2 0QQ ,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,12,15]]},"reference":[{"key":"2025030417575981200_r1","doi-asserted-by":"crossref","first-page":"2921","DOI":"10.1111\/ajt.13347","article-title":"Rates and determinants of progression to graft failure in kidney allograft recipients with de novo donor-specific antibody","volume":"15","author":"Wiebe","year":"2015","journal-title":"Am. J. Transplant."},{"key":"2025030417575981200_r2","doi-asserted-by":"crossref","first-page":"30","DOI":"10.1111\/nyas.12034","article-title":"Clinical relevance of antibody development in renal transplantation","volume":"1283","author":"Mehra","year":"2013","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2025030417575981200_r3","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/S0140-6736(12)61265-3","article-title":"Antibody-mediated vascular rejection of kidney allografts: a population-based study","volume":"381","author":"Lefaucheur","year":"2013","journal-title":"Lancet"},{"key":"2025030417575981200_r4","doi-asserted-by":"crossref","first-page":"769","DOI":"10.1016\/j.healun.2013.05.012","article-title":"Pathologic classification of antibody-mediated rejection correlates with donor-specific antibodies and endothelial cell activation","volume":"32","author":"Tible","year":"2013","journal-title":"J. Heart Lung Transplant."},{"key":"2025030417575981200_r5","doi-asserted-by":"crossref","first-page":"1398","DOI":"10.1681\/ASN.2009101065","article-title":"Preexisting donor-specific HLA antibodies predict outcome in kidney transplantation","volume":"21","author":"Lefaucheur","year":"2010","journal-title":"J. Am. Soc. 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J. Transplant."},{"key":"2025030417575981200_r9","doi-asserted-by":"crossref","first-page":"575","DOI":"10.3389\/fimmu.2016.00575","article-title":"HLA mismatching strategies for solid organ transplantation - a balancing act","volume":"7","author":"Zachary","year":"2016","journal-title":"Front. Immunol."},{"key":"2025030417575981200_r10","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1016\/S0140-6736(96)02296-9","article-title":"Association between specific HLA combinations and probability of kidney allograft loss: the taboo concept","volume":"348","author":"Doxiadis","year":"1996","journal-title":"Lancet"},{"key":"2025030417575981200_r11","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1084\/jem.172.6.1547","article-title":"T cell requirements for the rejection of renal allografts bearing an isolated class I MHC disparity","volume":"172","author":"Gracie","year":"1990","journal-title":"J. Exp. 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Res."},{"key":"2025030417575981200_r24","doi-asserted-by":"crossref","first-page":"14330","DOI":"10.1073\/pnas.96.25.14330","article-title":"Molecular dynamics and free-energy calculations applied to affinity maturation in antibody 48G7","volume":"96","author":"Chong","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025030417575981200_r25","doi-asserted-by":"crossref","first-page":"1171","DOI":"10.1038\/nbt1336","article-title":"Computational design of antibody-affinity improvement beyond in vivo maturation","volume":"25","author":"Lippow","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"2025030417575981200_r26","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1146\/annurev.bb.19.060190.001505","article-title":"Electrostatic interactions in macromolecules: theory and applications","volume":"19","author":"Sharp","year":"1990","journal-title":"Annu. Rev. Biophys. Biophys. 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Cryst."},{"key":"2025030417575981200_r40","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1038\/356083a0","article-title":"Assessment of protein models with three-dimensional profiles","volume":"356","author":"L\u00fcthy","year":"1992","journal-title":"Nature"},{"key":"2025030417575981200_r41","doi-asserted-by":"crossref","first-page":"2507","DOI":"10.1110\/ps.062416606","article-title":"Statistical potential for assessment and prediction of protein structures","volume":"15","author":"Shen","year":"2006","journal-title":"Protein Sci."},{"key":"2025030417575981200_r42","doi-asserted-by":"crossref","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","article-title":"Clustal W and clustal X version 2.0","volume":"23","author":"Larkin","year":"2007","journal-title":"Bioinformatics"},{"key":"2025030417575981200_r43","doi-asserted-by":"crossref","first-page":"525","DOI":"10.1021\/ct100578z","article-title":"PROPKA3: consistent treatment of internal and surface residues in empirical pKa predictions","volume":"7","author":"Olsson","year":"2011","journal-title":"J. Chem. Theory Comput."},{"key":"2025030417575981200_r44","doi-asserted-by":"crossref","first-page":"1978","DOI":"10.1021\/j100058a043","article-title":"Accurate calculation of hydration free energies using macroscopic solvent models","volume":"98","author":"Sitkoff","year":"1994","journal-title":"J. Phys. 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J. Quantum Chem."},{"key":"2025030417575981200_r49","doi-asserted-by":"crossref","first-page":"172","DOI":"10.1038\/ng.3176","article-title":"High-density mapping of the MHC identifies a shared role for HLA-DRB1*01:03 in inflammatory bowel diseases and heterozygous advantage in ulcerative colitis","volume":"47","year":"2015","journal-title":"Nat. Genet."},{"issue":"S14","key":"2025030417575981200_r50","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1002\/qua.560320814","article-title":"Molecular similarity based on electrostatic potential and electric field","volume":"32","author":"Hodgkin","year":"1987","journal-title":"Int. J. Quantum Chem."},{"key":"2025030417575981200_r51","volume-title":"R: A Language and Environment for Statistical Computing.","author":"R. 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Med."},{"key":"2025030417575981200_r55","doi-asserted-by":"crossref","first-page":"490","DOI":"10.4049\/jimmunol.137.2.490","article-title":"Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression","volume":"137","author":"Edwards","year":"1986","journal-title":"J. Immunol."},{"key":"2025030417575981200_r56","first-page":"247","article-title":"Control of MHC class II gene expression in autoimmune, infectious, and neoplastic diseases","volume":"13","author":"Guardiola","year":"1993","journal-title":"Crit. Rev. Immunol."},{"key":"2025030417575981200_r57","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/tri.12316","article-title":"DQ molecules are the principal stimulators of de novo donor-specific antibodies in nonsensitized pediatric recipients receiving a first kidney transplant","volume":"27","author":"Tagliamacco","year":"2014","journal-title":"Transpl. 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