{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T11:20:56Z","timestamp":1790680856247,"version":"4.1.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2018,10,1]],"date-time":"2018-10-01T00:00:00Z","timestamp":1538352000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,10,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Translating studies on T cell function and modulation from mouse models to humans requires extrapolating in vivo results on mouse T cell responses in lymphoid organs (spleen and lymph nodes [LN]) to human peripheral blood T cells. However, our understanding of T cell responses in human lymphoid sites and their relation to peripheral blood remains sparse. In this study, we used a unique human tissue resource to study human T cells in different anatomical compartments within individual donors and identify a subset of memory CD8+ T cells in LN, which maintain a distinct differentiation and functional profile compared with memory CD8+ T cells in blood, spleen, bone marrow, and lungs. Whole-transcriptome and high-dimensional cytometry by time-of-flight profiling reveals that LN memory CD8+ T cells express signatures of quiescence and self-renewal compared with corresponding populations in blood, spleen, bone marrow, and lung. LN memory T cells exhibit a distinct transcriptional signature, including expression of stem cell\u2013associated transcription factors TCF-1 and LEF-1, T follicular helper cell markers CXCR5 and CXCR4, and reduced expression of effector molecules. LN memory T cells display high homology to a subset of mouse CD8+ T cells identified in chronic infection models that respond to checkpoint blockade immunotherapy. Functionally, human LN memory T cells exhibit increased proliferation to TCR-mediated stimulation and maintain higher TCR clonal diversity compared with memory T cells from blood and other sites. These findings establish human LN as reservoirs for memory T cells with high capacities for expansion and diverse recognition and important targets for immunotherapies.<\/jats:p>","DOI":"10.4049\/jimmunol.1800716","type":"journal-article","created":{"date-parts":[[2018,8,15]],"date-time":"2018-08-15T10:17:20Z","timestamp":1534328240000},"page":"2132-2140","source":"Crossref","is-referenced-by-count":78,"title":["Human Lymph Nodes Maintain TCF-1hi Memory T Cells with High Functional Potential and Clonal Diversity throughout Life"],"prefix":"10.1093","volume":"201","author":[{"given":"Michelle","family":"Miron","sequence":"first","affiliation":[{"name":"Columbia Center for Translational Immunology, Columbia University Medical Center , New York, NY 10032"},{"name":"Department of Microbiology and Immunology, Columbia University , New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brahma V","family":"Kumar","sequence":"additional","affiliation":[{"name":"Columbia Center for Translational Immunology, Columbia University Medical Center , New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenzhao","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomer","family":"Granot","sequence":"additional","affiliation":[{"name":"Columbia Center for Translational Immunology, Columbia University Medical Center , New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dustin J","family":"Carpenter","sequence":"additional","affiliation":[{"name":"Columbia Center for Translational Immunology, Columbia University Medical Center , New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Senda","sequence":"additional","affiliation":[{"name":"Columbia Center for Translational Immunology, Columbia University Medical Center , New York, NY 10032"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8991-0673","authenticated-orcid":false,"given":"Dora","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania , Philadelphia, PA 19102"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2266-0355","authenticated-orcid":false,"given":"Aaron M","family":"Rosenfeld","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Drexel University , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8968-096X","authenticated-orcid":false,"given":"Bochao","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biomedical Engineering, Drexel University , Philadelphia, PA 19104"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1857-5190","authenticated-orcid":false,"given":"Harvey","family":"Lerner","sequence":"additional","affiliation":[{"name":"LiveOnNY , New York, NY 10001"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4706-8884","authenticated-orcid":false,"given":"Amy L","family":"Friedman","sequence":"additional","affiliation":[{"name":"LiveOnNY , New York, NY 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