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In this study, we examined the in vivo role of surface IgD in human B cell homeostasis and Ab responses in four individuals with heterozygous nonsense mutations in IGHD. All IGHD heterozygous individuals had normal numbers of B cells and serum Igs and did not show signs of immunodeficiency or immune dysregulation. IgD+ and IgD\u2212 naive mature B cells were present in equal numbers and showed similar immunophenotypes, except for decreased expression of CD79b in the IgD\u2212 subset. Furthermore, both IgD+ and IgD\u2212 naive mature B cells had normal replication histories and similar capacities to differentiate into plasma cells upon in vitro stimulation, and Ig class\u2013switched memory B cells showed similar levels of somatic hypermutations. Thus, human B cells lacking IgD expression develop normally and generate immunological memory in vivo, suggesting that surface IgD might function more restrictedly in regulating of B cell activation to specific antigenic structures.<\/jats:p>","DOI":"10.4049\/jimmunol.1800767","type":"journal-article","created":{"date-parts":[[2018,8,24]],"date-time":"2018-08-24T12:45:46Z","timestamp":1535114746000},"page":"1928-1935","source":"Crossref","is-referenced-by-count":11,"title":["Absence of Surface IgD Does Not Impair Naive B Cell Homeostasis or Memory B Cell Formation in\n                    <i>IGHD<\/i>\n                    Haploinsufficient Humans"],"prefix":"10.1093","volume":"201","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0634-005X","authenticated-orcid":false,"given":"Jana","family":"Nechvatalova","sequence":"first","affiliation":[{"name":"Department of Clinical Immunology and Allergology, St. Anne\u2019s University Hospital in Brno , 65691 Brno ,","place":["the Czech Republic"]},{"name":"Faculty of Medicine, Masaryk University , 62500 Brno ,","place":["the Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophinus J W","family":"Bartol","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus MC, University Medical Center , 3015CN Rotterdam ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zita","family":"Chovancova","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Allergology, St. Anne\u2019s University Hospital in Brno , 65691 Brno ,","place":["the Czech Republic"]},{"name":"Faculty of Medicine, Masaryk University , 62500 Brno ,","place":["the Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louis","family":"Boon","sequence":"additional","affiliation":[{"name":"Bioceros , 3584CM Utrecht ,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2122-7784","authenticated-orcid":false,"given":"Marcela","family":"Vlkova","sequence":"additional","affiliation":[{"name":"Department of Clinical Immunology and Allergology, St. Anne\u2019s University Hospital in Brno , 65691 Brno ,","place":["the Czech Republic"]},{"name":"Faculty of Medicine, Masaryk University , 62500 Brno ,","place":["the Czech Republic"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4161-1919","authenticated-orcid":false,"given":"Menno C","family":"van Zelm","sequence":"additional","affiliation":[{"name":"Department of Immunology, Erasmus MC, University Medical Center , 3015CN Rotterdam ,","place":["the Netherlands"]},{"name":"Department of Immunology and Pathology, Central Clinical School, Monash University and The Alfred Hospital , Melbourne, Victoria 3004 ,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2018,10,1]]},"reference":[{"key":"2025030417573310200_r1","doi-asserted-by":"crossref","first-page":"575","DOI":"10.1038\/302575a0","article-title":"Somatic generation of antibody diversity","volume":"302","author":"Tonegawa","year":"1983","journal-title":"Nature"},{"key":"2025030417573310200_r2","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1016\/S0092-8674(00)80373-6","article-title":"In vivo ablation of surface immunoglobulin on mature B cells by inducible gene targeting results in rapid cell death","volume":"90","author":"Lam","year":"1997","journal-title":"Cell"},{"key":"2025030417573310200_r3","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1111\/j.0105-2896.2003.097.x","article-title":"Positive selection and lineage commitment during peripheral B-lymphocyte development","volume":"197","author":"Pillai","year":"2004","journal-title":"Immunol. 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