{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T03:53:47Z","timestamp":1768449227699,"version":"3.49.0"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Adenoviruses are a major cause of infectious mortality in children following allogeneic hematopoietic stem cell transplantation, with adoptive transfer of adenovirus-specific T cells being an effective therapeutic approach. We have previously shown that T cells specific for the peptide epitope LTDLGQNLLY were protective. In this study, we aimed to establish a viral dissemination assay to measure the antiviral capacity of T cells specific for this and other peptide epitopes in an infectious setting. We used replication-competent adenovirus 11 (Ad11pGFP) and adenovirus 5 containing adenovirus 35 fiber (Ad5F35GFP) viruses and T cells specific for HLA-A*01\u2013restricted LTDLGQNLLY, HLA-B*07\u2013restricted KPYSGTAYNAL, and HLA-A*02\u2013restricted LLDQLIEEV peptide epitopes. T cells in PBMC from healthy donors were expanded with peptide and IL-2 or treated with IL-2 alone to serve as nonstimulated control cells, and then these expanded or nonstimulated CD8+ cells were purified and cocultured with autologous monocytes infected with adenovirus at low multiplicity of infection. After 3 d, the number of infected GFP+ monocytes and, hence, viral dissemination was quantified by flow cytometry. T cells expanded with LTDLGQNLLY peptide from multiple HLA-A*01+ donors prevented adenovirus dissemination, and nonstimulated T cells did not prevent dissemination, thus, indicating that LTDLGQNLLY-specific T cells have high antiviral capacity. Similarly, expanded KPYSGTAYNAL- and LLDQLIEEV-specific T cells could prevent viral dissemination. However, the frequency of expanded T cells specific for these last two epitopes was variable between donors with consequent variable prevention of adenoviral dissemination. Taken together, we demonstrate that T cells specific for three peptide epitopes, from both structural and nonstructural proteins, can prevent adenoviral dissemination and provide a novel method to measure the antiviral capacity of adenovirus-specific T cell responses.<\/jats:p>","DOI":"10.4049\/jimmunol.1801003","type":"journal-article","created":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T20:49:23Z","timestamp":1544215763000},"page":"618-624","source":"Crossref","is-referenced-by-count":9,"title":["Measuring Antiviral Capacity of T Cell Responses to Adenovirus"],"prefix":"10.1093","volume":"202","author":[{"given":"Anna","family":"Keib","sequence":"first","affiliation":[{"name":"Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital T\u00fcbingen \u00a0 72076, Tubingen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7719-3512","authenticated-orcid":false,"given":"Ya-Fang","family":"Mei","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Umea University , Umea 90185 ,","place":["Sweden"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3978-778X","authenticated-orcid":false,"given":"Luka","family":"Cicin-Sain","sequence":"additional","affiliation":[{"name":"Helmholtz Center for Infection Research , Braunschweig 38124 ,","place":["Germany"]},{"name":"German Center for Infection Research , partner sites T\u00fcbingen 72076, Munich 81675, and Braunschweig 38124 ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dirk H","family":"Busch","sequence":"additional","affiliation":[{"name":"German Center for Infection Research , partner sites T\u00fcbingen 72076, Munich 81675, and Braunschweig 38124 ,","place":["Germany"]},{"name":"Institute for Medical Microbiology, Immunology and Hygiene, Technical University Munich , Munich 81675 ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin M","family":"Dennehy","sequence":"additional","affiliation":[{"name":"Institute for Medical Virology and Epidemiology of Viral Diseases, University Hospital T\u00fcbingen \u00a0 72076, Tubingen ,","place":["Germany"]},{"name":"German Center for Infection Research , partner sites T\u00fcbingen 72076, Munich 81675, and Braunschweig 38124 ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,1,15]]},"reference":[{"key":"2025030417560103000_r1","doi-asserted-by":"crossref","first-page":"244","DOI":"10.1080\/10428190600881157","article-title":"Adenovirus infection after allogeneic stem cell transplantation","volume":"48","author":"Feuchtinger","year":"2007","journal-title":"Leuk. 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