{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T05:13:32Z","timestamp":1772255612786,"version":"3.50.1"},"reference-count":76,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2019,2,1]],"date-time":"2019-02-01T00:00:00Z","timestamp":1548979200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>CMV is a prevalent human pathogen. The virus cannot be eliminated from the body, but is kept in check by CMV-specific T cells. Patients with an insufficient T cell response, such as transplant recipients, are at high risk of developing CMV disease. However, the CMV-specific T cell repertoire is complex, and it is not yet clear which T cells protect best against virus reactivation and disease. In this study, we present a highly resolved characterization of CMV-specific human CD8+ T cells based on enrichment by specific peptide stimulation and mRNA sequencing of their TCR \u03b2-chains (TCR\u03b2). Our analysis included recently identified T cell epitopes restricted through HLA-C, whose presentation is resistant to viral immunomodulation, and well-studied HLA-B\u2013restricted epitopes. In eight healthy virus carriers, we identified a total of 1052 CMV-specific TCR\u03b2 sequences. HLA-C\u2013restricted, CMV-specific TCR\u03b2 clonotypes dominated the ex vivo T cell response and contributed the highest-frequency clonotype of the entire repertoire in two of eight donors. We analyzed sharing and similarity of CMV-specific TCR\u03b2 sequences and identified 63 public or related sequences belonging to 17 public TCR\u03b2 families. In our cohort, and in an independent cohort of 352 donors, the cumulative frequency of these public TCR\u03b2 family members was a highly discriminatory indicator of carrying both CMV infection and the relevant HLA type. Based on these findings, we propose CMV-specific TCR\u03b2 signatures as a biomarker for an antiviral T cell response to identify patients in need of treatment and to guide future development of immunotherapy.<\/jats:p>","DOI":"10.4049\/jimmunol.1801401","type":"journal-article","created":{"date-parts":[[2018,12,26]],"date-time":"2018-12-26T15:15:15Z","timestamp":1545837315000},"page":"979-990","source":"Crossref","is-referenced-by-count":37,"title":["Antigen-Specific TCR Signatures of Cytomegalovirus Infection"],"prefix":"10.1093","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1028-0128","authenticated-orcid":false,"given":"Alina","family":"Huth","sequence":"first","affiliation":[{"name":"German Center for Infection Research Group Host Control of Viral Latency and Reactivation, Research Unit Gene Vectors, Helmholtz Center Munich , 81377 Munich ,","place":["Germany"]},{"name":"Deutsches Zentrum f\u00fcr Infektionsforschung , 81377 Munich ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0572-5188","authenticated-orcid":false,"given":"Xiaoling","family":"Liang","sequence":"additional","affiliation":[{"name":"German Center for Infection Research Group Host Control of Viral Latency and Reactivation, Research Unit Gene Vectors, Helmholtz Center Munich , 81377 Munich ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Krebs","sequence":"additional","affiliation":[{"name":"Laboratory for Functional Genome Analysis, Gene Center, Ludwig Maximilian University of Munich , 81377 Munich ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helmut","family":"Blum","sequence":"additional","affiliation":[{"name":"Laboratory for Functional Genome Analysis, Gene Center, Ludwig Maximilian University of Munich , 81377 Munich ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8044-5146","authenticated-orcid":false,"given":"Andreas","family":"Moosmann","sequence":"additional","affiliation":[{"name":"German Center for Infection Research Group Host Control of Viral Latency and Reactivation, Research Unit Gene Vectors, Helmholtz Center Munich , 81377 Munich ,","place":["Germany"]},{"name":"Deutsches Zentrum f\u00fcr Infektionsforschung , 81377 Munich ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,2,1]]},"reference":[{"key":"2025030417560567400_r1","doi-asserted-by":"crossref","first-page":"1232","DOI":"10.1182\/blood.V97.5.1232","article-title":"Direct visualization of cytomegalovirus-specific T-cell reconstitution after allogeneic stem cell transplantation","volume":"97","author":"Cwynarski","year":"2001","journal-title":"Blood"},{"key":"2025030417560567400_r2","doi-asserted-by":"crossref","first-page":"3830","DOI":"10.1182\/blood.V99.10.3830","article-title":"Sensitive detection of human cytomegalovirus peptide-specific cytotoxic T-lymphocyte responses by interferon-gamma-enzyme-linked immunospot assay and flow cytometry in healthy individuals and in patients after allogeneic stem cell transplantation","volume":"99","author":"Hebart","year":"2002","journal-title":"Blood"},{"key":"2025030417560567400_r3","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1182\/asheducation-2011.1.305","article-title":"Complications, diagnosis, management, and prevention of CMV infections: current and future","volume":"2011","author":"Boeckh","year":"2011","journal-title":"Hematology (Am. Soc. Hematol. Educ. Program)"},{"key":"2025030417560567400_r4","article-title":"Definitions of resistant and refractory cytomegalovirus infection and disease in transplant recipients for use in clinical trials","author":"Chemaly","year":"2018","journal-title":"Clin. Infect. Dis."},{"key":"2025030417560567400_r5","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.1172\/JCI90599","article-title":"Immunotherapy for transplantation-associated viral infections","volume":"127","author":"Roddie","year":"2017","journal-title":"J. Clin. Invest."},{"key":"2025030417560567400_r6","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.1016\/j.bbmt.2018.03.028","article-title":"Control of cytomegalovirus viremia after allogeneic stem cell transplantation: a review on CMV-specific T cell reconstitution","volume":"24","author":"van der Heiden","year":"2018","journal-title":"Biol. Blood Marrow Transplant."},{"key":"2025030417560567400_r7","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1128\/CMR.00034-08","article-title":"Immunobiology of human cytomegalovirus: from bench to bedside","volume":"22","author":"Crough","year":"2009","journal-title":"Clin. Microbiol. Rev."},{"key":"2025030417560567400_r8","doi-asserted-by":"crossref","first-page":"139","DOI":"10.1038\/cmi.2014.105","article-title":"Classical and non-classical MHC I molecule manipulation by human cytomegalovirus: so many targets\u2014but how many arrows in the quiver?","volume":"12","author":"Halenius","year":"2015","journal-title":"Cell. Mol. Immunol."},{"key":"2025030417560567400_r9","doi-asserted-by":"crossref","first-page":"140","DOI":"10.1007\/s12026-012-8304-8","article-title":"Diverse immune evasion strategies by human cytomegalovirus","volume":"54","author":"Noriega","year":"2012","journal-title":"Immunol. Res."},{"key":"2025030417560567400_r10","doi-asserted-by":"crossref","first-page":"3993","DOI":"10.1038\/srep03993","article-title":"Molecular imprint of exposure to naturally occurring genetic variants of human cytomegalovirus on the T cell repertoire","volume":"4","author":"Smith","year":"2014","journal-title":"Sci. Rep."},{"key":"2025030417560567400_r11","doi-asserted-by":"crossref","first-page":"8179","DOI":"10.1128\/JVI.73.10.8179-8184.1999","article-title":"Target structures of the CD8(+)-T-cell response to human cytomegalovirus: the 72-kilodalton major immediate-early protein revisited","volume":"73","author":"Kern","year":"1999","journal-title":"J. Virol."},{"key":"2025030417560567400_r12","doi-asserted-by":"crossref","first-page":"7569","DOI":"10.1128\/jvi.70.11.7569-7579.1996","article-title":"The human cytotoxic T-lymphocyte (CTL) response to cytomegalovirus is dominated by structural protein pp65: frequency, specificity, and T-cell receptor usage of pp65-specific CTL","volume":"70","author":"Wills","year":"1996","journal-title":"J. Virol."},{"key":"2025030417560567400_r13","doi-asserted-by":"crossref","first-page":"1075","DOI":"10.1182\/blood-2003-06-1937","article-title":"Immune evasion proteins of human cytomegalovirus do not prevent a diverse CD8+ cytotoxic T-cell response in natural infection","volume":"104","author":"Manley","year":"2004","journal-title":"Blood"},{"key":"2025030417560567400_r14","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1084\/jem.20042384","article-title":"Protection from cytomegalovirus after transplantation is correlated with immediate early 1-specific CD8 T cells","volume":"201","author":"Bunde","year":"2005","journal-title":"J. Exp. Med."},{"key":"2025030417560567400_r15","doi-asserted-by":"crossref","first-page":"10143","DOI":"10.1128\/JVI.00688-08","article-title":"Expansion of human cytomegalovirus (HCMV) immediate-early 1-specific CD8+ T cells and control of HCMV replication after allogeneic stem cell transplantation","volume":"82","author":"Sacre","year":"2008","journal-title":"J. Virol."},{"key":"2025030417560567400_r16","doi-asserted-by":"crossref","first-page":"3102","DOI":"10.1128\/jvi.61.10.3102-3108.1987","article-title":"CD8-positive T lymphocytes specific for murine cytomegalovirus immediate-early antigens mediate protective immunity","volume":"61","author":"Reddehase","year":"1987","journal-title":"J. Virol."},{"key":"2025030417560567400_r17","doi-asserted-by":"crossref","first-page":"e1003383","DOI":"10.1371\/journal.ppat.1003383","article-title":"Presentation of an immunodominant immediate-early CD8+ T cell epitope resists human cytomegalovirus immunoevasion","volume":"9","author":"Ameres","year":"2013","journal-title":"PLoS Pathog."},{"key":"2025030417560567400_r18","doi-asserted-by":"crossref","first-page":"5894","DOI":"10.4049\/jimmunol.1302281","article-title":"CD8 T cell-evasive functions of human cytomegalovirus display pervasive MHC allele specificity, complementarity, and cooperativity","volume":"192","author":"Ameres","year":"2014","journal-title":"J. Immunol."},{"key":"2025030417560567400_r19","doi-asserted-by":"crossref","first-page":"1776","DOI":"10.3389\/fimmu.2017.01776","article-title":"Cytomegalovirus-specific T cells restricted by HLA-cw*0702 increase markedly with age and dominate the CD8+ T-cell repertoire in older people","volume":"8","author":"Hosie","year":"2017","journal-title":"Front. Immunol."},{"key":"2025030417560567400_r20","doi-asserted-by":"crossref","first-page":"984","DOI":"10.1038\/ni.2097","article-title":"Human cytomegalovirus microRNA miR-US4-1 inhibits CD8(+) T cell responses by targeting the aminopeptidase ERAP1","volume":"12","author":"Kim","year":"2011","journal-title":"Nat. Immunol."},{"key":"2025030417560567400_r21","doi-asserted-by":"crossref","first-page":"422","DOI":"10.1126\/science.8211163","article-title":"Expression of two T cell receptor alpha chains: dual receptor T cells","volume":"262","author":"Padovan","year":"1993","journal-title":"Science"},{"key":"2025030417560567400_r22","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1038\/334395a0","article-title":"T-cell antigen receptor genes and T-cell recognition. [Published erratum appears in 1988 Nature 335: 744]","volume":"334","author":"Davis","year":"1988","journal-title":"Nature"},{"key":"2025030417560567400_r23","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1034\/j.1600-065X.2002.19003.x","article-title":"Sequence variation in the human T-cell receptor loci","volume":"190","author":"Mackelprang","year":"2002","journal-title":"Immunol. Rev."},{"key":"2025030417560567400_r24","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1038\/384134a0","article-title":"Structure of the complex between human T-cell receptor, viral peptide and HLA-A2","volume":"384","author":"Garboczi","year":"1996","journal-title":"Nature"},{"key":"2025030417560567400_r25","doi-asserted-by":"crossref","first-page":"958","DOI":"10.1126\/science.286.5441.958","article-title":"A direct estimate of the human alphabeta T cell receptor diversity","volume":"286","author":"Arstila","year":"1999","journal-title":"Science"},{"key":"2025030417560567400_r26","doi-asserted-by":"crossref","first-page":"4099","DOI":"10.1182\/blood-2009-04-217604","article-title":"Comprehensive assessment of T-cell receptor beta-chain diversity in alphabeta T cells","volume":"114","author":"Robins","year":"2009","journal-title":"Blood"},{"key":"2025030417560567400_r27","doi-asserted-by":"crossref","first-page":"47ra64","DOI":"10.1126\/scitranslmed.3001442","article-title":"Overlap and effective size of the human CD8+ T cell receptor repertoire","volume":"2","author":"Robins","year":"2010","journal-title":"Sci. Transl. Med."},{"key":"2025030417560567400_r28","doi-asserted-by":"crossref","first-page":"13139","DOI":"10.1073\/pnas.1409155111","article-title":"Diversity and clonal selection in the human T-cell repertoire","volume":"111","author":"Qi","year":"2014","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025030417560567400_r29","doi-asserted-by":"crossref","first-page":"7039","DOI":"10.4049\/jimmunol.1003309","article-title":"Large TCR diversity of virus-specific CD8 T cells provides the mechanistic basis for massive TCR renewal after antigen exposure","volume":"186","author":"Miconnet","year":"2011","journal-title":"J. Immunol."},{"key":"2025030417560567400_r30","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1084\/jem.20051357","article-title":"Avidity for antigen shapes clonal dominance in CD8+ T cell populations specific for persistent DNA viruses","volume":"202","author":"Price","year":"2005","journal-title":"J. Exp. Med."},{"key":"2025030417560567400_r31","doi-asserted-by":"crossref","first-page":"7853","DOI":"10.4049\/jimmunol.181.11.7853","article-title":"TCR beta-chain sharing in human CD8+ T cell responses to cytomegalovirus and EBV","volume":"181","author":"Venturi","year":"2008","journal-title":"J. Immunol."},{"key":"2025030417560567400_r32","doi-asserted-by":"crossref","first-page":"1817","DOI":"10.1101\/gr.092924.109","article-title":"Profiling the T-cell receptor beta-chain repertoire by massively parallel sequencing","volume":"19","author":"Freeman","year":"2009","journal-title":"Genome Res."},{"key":"2025030417560567400_r33","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1038\/nature22383","article-title":"Quantifiable predictive features define epitope-specific T cell receptor repertoires","volume":"547","author":"Dash","year":"2017","journal-title":"Nature"},{"key":"2025030417560567400_r34","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1038\/nature22976","article-title":"Identifying specificity groups in the T cell receptor repertoire","volume":"547","author":"Glanville","year":"2017","journal-title":"Nature"},{"key":"2025030417560567400_r35","doi-asserted-by":"crossref","first-page":"e1002889","DOI":"10.1371\/journal.ppat.1002889","article-title":"Deep sequencing of antiviral T-cell responses to HCMV and EBV in humans reveals a stable repertoire that is maintained for many years","volume":"8","author":"Klarenbeek","year":"2012","journal-title":"PLoS Pathog."},{"key":"2025030417560567400_r36","doi-asserted-by":"crossref","first-page":"3835","DOI":"10.1182\/blood-2015-03-631853","article-title":"CMV reactivation drives posttransplant T-cell reconstitution and results in defects in the underlying TCR\u03b2 repertoire","volume":"125","author":"Suessmuth","year":"2015","journal-title":"Blood"},{"key":"2025030417560567400_r37","doi-asserted-by":"crossref","first-page":"1755","DOI":"10.1182\/blood.V100.5.1755.h81702001755_1755_1764","article-title":"B cells immortalized by a mini-Epstein-Barr virus encoding a foreign antigen efficiently reactivate specific cytotoxic T cells","volume":"100","author":"Moosmann","year":"2002","journal-title":"Blood"},{"key":"2025030417560567400_r38","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/nmeth.3364","article-title":"MiXCR: software for comprehensive adaptive immunity profiling","volume":"12","author":"Bolotin","year":"2015","journal-title":"Nat. Methods"},{"key":"2025030417560567400_r39","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1186\/s12967-018-1498-3","article-title":"Clinical-grade generation of peptide-stimulated CMV\/EBV-specific T cells from G-CSF mobilized stem cell grafts","volume":"16","author":"Gary","year":"2018","journal-title":"J. Transl. Med."},{"key":"2025030417560567400_r40","doi-asserted-by":"crossref","first-page":"1622","DOI":"10.1038\/mt.2012.130","article-title":"Rapidly generated multivirus-specific cytotoxic T lymphocytes for the prophylaxis and treatment of viral infections","volume":"20","author":"Gerdemann","year":"2012","journal-title":"Mol. Ther."},{"key":"2025030417560567400_r41","doi-asserted-by":"crossref","first-page":"1313","DOI":"10.1016\/j.humimm.2013.06.025","article-title":"Six-locus high resolution HLA haplotype frequencies derived from mixed-resolution DNA typing for the entire US donor registry","volume":"74","author":"Gragert","year":"2013","journal-title":"Hum. Immunol."},{"key":"2025030417560567400_r42","doi-asserted-by":"crossref","first-page":"2099","DOI":"10.1128\/JVI.73.3.2099-2108.1999","article-title":"The memory cytotoxic T-lymphocyte (CTL) response to human cytomegalovirus infection contains individual peptide-specific CTL clones that have undergone extensive expansion in vivo","volume":"73","author":"Weekes","year":"1999","journal-title":"J. Virol."},{"key":"2025030417560567400_r43","doi-asserted-by":"crossref","first-page":"8","DOI":"10.1111\/j.0105-2896.2005.00309.x","article-title":"The seven dirty little secrets of major histocompatibility complex class I antigen processing","volume":"207","author":"Yewdell","year":"2005","journal-title":"Immunol. Rev."},{"key":"2025030417560567400_r44","doi-asserted-by":"crossref","first-page":"598","DOI":"10.3389\/fimmu.2015.00598","article-title":"A diverse repertoire of CD4 T cells targets the immediate-early 1 protein of human cytomegalovirus","volume":"6","author":"Ameres","year":"2015","journal-title":"Front. Immunol."},{"key":"2025030417560567400_r45","doi-asserted-by":"crossref","first-page":"2110","DOI":"10.1002\/eji.200425936","article-title":"Selection of CMV-specific CD8+ and CD4+ T cells by mini-EBV-transformed B cell lines","volume":"35","author":"Wiesner","year":"2005","journal-title":"Eur. J. Immunol."},{"key":"2025030417560567400_r46","doi-asserted-by":"crossref","first-page":"2742","DOI":"10.4049\/jimmunol.1102675","article-title":"The impact of a large and frequent deletion in the human TCR \u03b2 locus on antiviral immunity","volume":"188","author":"Brennan","year":"2012","journal-title":"J. Immunol."},{"key":"2025030417560567400_r47","doi-asserted-by":"crossref","first-page":"2842","DOI":"10.1111\/ajt.12431","article-title":"TCR repertoire analysis by next generation sequencing allows complex differential diagnosis of T cell-related pathology","volume":"13","author":"Dziubianau","year":"2013","journal-title":"Am. J. Transplant."},{"key":"2025030417560567400_r48","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1038\/ng.3822","article-title":"Immunosequencing identifies signatures of cytomegalovirus exposure history and HLA-mediated effects on the T cell repertoire","volume":"49","author":"Emerson","year":"2017","journal-title":"Nat. Genet."},{"key":"2025030417560567400_r49","doi-asserted-by":"crossref","first-page":"673","DOI":"10.1084\/jem.20050882","article-title":"Broadly targeted human cytomegalovirus-specific CD4+ and CD8+ T cells dominate the memory compartments of exposed subjects","volume":"202","author":"Sylwester","year":"2005","journal-title":"J. Exp. Med."},{"key":"2025030417560567400_r50","doi-asserted-by":"crossref","first-page":"1827","DOI":"10.4049\/jimmunol.162.3.1827","article-title":"A re-evaluation of the frequency of CD8+ T cells specific for EBV in healthy virus carriers","volume":"162","author":"Tan","year":"1999","journal-title":"J. Immunol."},{"key":"2025030417560567400_r51","doi-asserted-by":"crossref","first-page":"569","DOI":"10.1016\/j.celrep.2017.03.072","article-title":"Sequence and structural analyses reveal distinct and highly diverse human CD8+ TCR repertoires to immunodominant viral antigens","volume":"19","author":"Chen","year":"2017","journal-title":"Cell Rep."},{"key":"2025030417560567400_r52","doi-asserted-by":"crossref","first-page":"79","DOI":"10.1084\/jem.181.1.79","article-title":"Human HLA-A0201-restricted cytotoxic T lymphocyte recognition of influenza A is dominated by T cells bearing the V beta 17 gene segment","volume":"181","author":"Lehner","year":"1995","journal-title":"J. Exp. Med."},{"key":"2025030417560567400_r53","doi-asserted-by":"crossref","first-page":"6804","DOI":"10.4049\/jimmunol.177.10.6804","article-title":"TCR alpha genes direct MHC restriction in the potent human T cell response to a class I-bound viral epitope","volume":"177","author":"Miles","year":"2006","journal-title":"J. Immunol."},{"key":"2025030417560567400_r54","doi-asserted-by":"crossref","first-page":"e0141561","DOI":"10.1371\/journal.pone.0141561","article-title":"Multiplex identification of antigen-specific T cell receptors using a combination of immune assays and immune receptor sequencing","volume":"10","author":"Klinger","year":"2015","journal-title":"PLoS One"},{"key":"2025030417560567400_r55","doi-asserted-by":"crossref","first-page":"6123","DOI":"10.4049\/jimmunol.175.9.6123","article-title":"Selection of T cell clones expressing high-affinity public TCRs within human cytomegalovirus-specific CD8 T cell responses","volume":"175","author":"Trautmann","year":"2005","journal-title":"J. Immunol."},{"key":"2025030417560567400_r56","doi-asserted-by":"crossref","first-page":"3063","DOI":"10.4049\/jimmunol.0903622","article-title":"Public TCR use by herpes simplex virus-2-specific human CD8 CTLs","volume":"184","author":"Dong","year":"2010","journal-title":"J. Immunol."},{"key":"2025030417560567400_r57","doi-asserted-by":"crossref","first-page":"2279","DOI":"10.1073\/pnas.96.5.2279","article-title":"Crossreactive recognition of viral, self, and bacterial peptide ligands by human class I-restricted cytotoxic T lymphocyte clonotypes: implications for molecular mimicry in autoimmune disease","volume":"96","author":"Misko","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025030417560567400_r58","doi-asserted-by":"crossref","first-page":"1358","DOI":"10.1182\/blood.V98.5.1358","article-title":"Tetramer-based quantification of cytomegalovirus (CMV)-specific CD8+ T lymphocytes in T-cell-depleted stem cell grafts and after transplantation may identify patients at risk for progressive CMV infection","volume":"98","author":"Gratama","year":"2001","journal-title":"Blood"},{"key":"2025030417560567400_r59","doi-asserted-by":"crossref","first-page":"3979","DOI":"10.1182\/blood-2003-12-4287","article-title":"Prompt versus preemptive intervention for EBV lymphoproliferative disease","volume":"103","author":"Wagner","year":"2004","journal-title":"Blood"},{"key":"2025030417560567400_r60","doi-asserted-by":"crossref","first-page":"319","DOI":"10.4049\/jimmunol.0803647","article-title":"Clonotype selection and composition of human CD8 T cells specific for persistent herpes viruses varies with differentiation but is stable over time","volume":"183","author":"Iancu","year":"2009","journal-title":"J. Immunol."},{"key":"2025030417560567400_r61","doi-asserted-by":"crossref","first-page":"931","DOI":"10.4049\/jimmunol.1202466","article-title":"CD8+ TCR repertoire formation is guided primarily by the peptide component of the antigenic complex","volume":"190","author":"Koning","year":"2013","journal-title":"J. Immunol."},{"key":"2025030417560567400_r62","doi-asserted-by":"crossref","first-page":"2038","DOI":"10.1002\/1529-0131(200109)44:9<2038::AID-ART353>3.0.CO;2-1","article-title":"Epitope specificity of clonally expanded populations of CD8+ T cells found within the joints of patients with inflammatory arthritis","volume":"44","author":"Fazou","year":"2001","journal-title":"Arthritis Rheum."},{"key":"2025030417560567400_r63","doi-asserted-by":"crossref","first-page":"2001","DOI":"10.4049\/jimmunol.165.4.2001","article-title":"Frequent contribution of T cell clonotypes with public TCR features to the chronic response against a dominant EBV-derived epitope: application to direct detection of their molecular imprint on the human peripheral T cell repertoire","volume":"165","author":"Lim","year":"2000","journal-title":"J. Immunol."},{"key":"2025030417560567400_r64","doi-asserted-by":"crossref","first-page":"3826","DOI":"10.4049\/jimmunol.175.6.3826","article-title":"CTL recognition of a bulged viral peptide involves biased TCR selection","volume":"175","author":"Miles","year":"2005","journal-title":"J. Immunol."},{"key":"2025030417560567400_r65","doi-asserted-by":"crossref","first-page":"946","DOI":"10.1038\/ni.3491","article-title":"Hydrophobic CDR3 residues promote the development of self-reactive T cells","volume":"17","author":"Stadinski","year":"2016","journal-title":"Nat. Immunol."},{"key":"2025030417560567400_r66","doi-asserted-by":"crossref","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform","volume":"79","author":"Kozich","year":"2013","journal-title":"Appl. Environ. Microbiol."},{"key":"2025030417560567400_r67","doi-asserted-by":"crossref","first-page":"e74231","DOI":"10.1371\/journal.pone.0074231","article-title":"Combining next-generation sequencing and immune assays: a novel method for identification of antigen-specific T cells","volume":"8","author":"Klinger","year":"2013","journal-title":"PLoS One"},{"key":"2025030417560567400_r68","doi-asserted-by":"crossref","first-page":"1867","DOI":"10.1002\/j.1460-2075.1990.tb08312.x","article-title":"Transcriptional and post-transcriptional regulation of TcR, CD4 and CD8 gene expression during activation of normal human T lymphocytes","volume":"9","author":"Paillard","year":"1990","journal-title":"EMBO J."},{"key":"2025030417560567400_r69","doi-asserted-by":"crossref","first-page":"e73648","DOI":"10.1371\/journal.pone.0073648","article-title":"MHC class II tetramers made from isolated recombinant \u03b1 and \u03b2 chains refolded with affinity-tagged peptides. [Published erratum appears in 2013 PLoS One. 8.]","volume":"8","author":"Braendstrup","year":"2013","journal-title":"PLoS One"},{"key":"2025030417560567400_r70","doi-asserted-by":"crossref","first-page":"e0193554","DOI":"10.1371\/journal.pone.0193554","article-title":"Characterization and clinical enrichment of HLA-C*07:02-restricted Cytomegalovirus-specific CD8+ T cells","volume":"13","author":"Schlott","year":"2018","journal-title":"PLoS One"},{"key":"2025030417560567400_r71","doi-asserted-by":"crossref","first-page":"2263","DOI":"10.4049\/jimmunol.1700242","article-title":"Peptide-MHC class I tetramers can fail to detect relevant functional T cell clonotypes and underestimate antigen-reactive T cell populations","volume":"200","author":"Rius","year":"2018","journal-title":"J. Immunol."},{"issue":"D1","key":"2025030417560567400_r72","doi-asserted-by":"crossref","first-page":"D419","DOI":"10.1093\/nar\/gkx760","article-title":"VDJdb: a curated database of T-cell receptor sequences with known antigen specificity","volume":"46","author":"Shugay","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"2025030417560567400_r73","doi-asserted-by":"crossref","first-page":"77","DOI":"10.1038\/icb.2016.71","article-title":"Maintenance of the EBV-specific CD8+ TCR\u03b1\u03b2 repertoire in immunosuppressed lung transplant recipients","volume":"95","author":"Nguyen","year":"2017","journal-title":"Immunol. Cell Biol."},{"key":"2025030417560567400_r74","doi-asserted-by":"crossref","first-page":"372","DOI":"10.1038\/nm.3100","article-title":"Quantitative assessment of T cell repertoire recovery after hematopoietic stem cell transplantation","volume":"19","author":"van Heijst","year":"2013","journal-title":"Nat. Med."},{"key":"2025030417560567400_r75","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1182\/blood-2009-07-234906","article-title":"Allo-HLA reactivity of virus-specific memory T cells is common","volume":"115","author":"Amir","year":"2010","journal-title":"Blood"},{"key":"2025030417560567400_r76","doi-asserted-by":"crossref","first-page":"6819","DOI":"10.4049\/jimmunol.0902233","article-title":"CMV-specific TCR-transgenic T cells for immunotherapy","volume":"183","author":"Schub","year":"2009","journal-title":"J. Immunol."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/202\/3\/979\/61873280\/ji1801401.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/202\/3\/979\/61873280\/ji1801401.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,4]],"date-time":"2025-03-04T14:22:33Z","timestamp":1741098153000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/202\/3\/979\/7953875"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,2,1]]},"references-count":76,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2019,2,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.1801401","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/450882","asserted-by":"object"}]},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2019,2]]},"published":{"date-parts":[[2019,2,1]]}}}