{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:16:31Z","timestamp":1789989391077,"version":"4.0.1"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Osteoarthritis (OA) is a degenerative joint disease that causes chronic disability among the elderly. Despite recent advances in symptomatic management of OA by pharmacological and surgical approaches, there remains a lack of optimal approaches to manage inflammation in the joints, which causes cartilage degradation and pain. In this study, we investigated the efficacy and underlying mechanisms of nicotine exposure in attenuating joint inflammation, cartilage degradation, and pain in a mouse model of OA. A mouse model of OA was induced by injection of monosodium iodoacetate into the knee joint. Cell culture models were also used to study the efficacy and underlying mechanisms of nicotine treatment in attenuating symptoms of OA. Nicotine treatment reduced mechanical allodynia, cartilage degradation, and the upregulation of matrix metalloproteinase-9 (MMP-9), a hallmark of joint inflammation in OA, in mice treated with monosodium iodoacetate. The effects of nicotine were abolished by the selective \u03b17 nicotinic acetylcholine receptor (nAChR) blocker, methyllycaconitine . In RAW264.7 cells and murine primary bone marrow\u2013derived macrophages, nicotine significantly inhibited MMP-9 production induced by LPS. In addition, nicotine significantly enhanced PI3K\/Akt and inhibited NF-\u03baB translocation from the cytosol to the nucleus in an \u03b17-nAChR\u2013dependent manner, suggesting that nicotine acts on \u03b17-nAChRs to inhibit MMP-9 production by macrophages through modulation of the PI3K\/Akt\u2013NF-\u03baB pathway. Our results provide novel evidence that nicotine can attenuate joint inflammation and pain in experimental OA via \u03b17-nAChRs. \u03b17-nAChR could thus serve as a highly promising target to manage joint inflammation and pain in OA.<\/jats:p>","DOI":"10.4049\/jimmunol.1801513","type":"journal-article","created":{"date-parts":[[2019,5,31]],"date-time":"2019-05-31T14:55:10Z","timestamp":1559314510000},"page":"485-492","source":"Crossref","is-referenced-by-count":59,"title":["Nicotine Attenuates Osteoarthritis Pain and Matrix Metalloproteinase-9 Expression via the \u03b17 Nicotinic Acetylcholine Receptor"],"prefix":"10.1093","volume":"203","author":[{"given":"Peng","family":"Teng","sequence":"first","affiliation":[{"name":"Department of Orthopedics, First Affiliated Hospital of Nanjing Medical University , Nanjing 210029 ,","place":["China"]},{"name":"Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University , Nanjing 210029 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, First Affiliated Hospital of Nanjing Medical University , Nanjing 210029 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, First Affiliated Hospital of Nanjing Medical University , Nanjing 210029 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First Affiliated Hospital of Nanjing Medical University , Nanjing 210029 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Tao","family":"Liu","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Neurodegeneration, Department of Pharmacology, Nanjing Medical University , Nanjing 210029 ,","place":["China"]},{"name":"Department of Pharmacy, Sir Run Run Shaw Hospital, Nanjing Medical University , Nanjing 210029 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7258-7865","authenticated-orcid":false,"given":"Jun","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Orthopedics, First Affiliated Hospital of Nanjing Medical University , Nanjing 210029 ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,7,15]]},"reference":[{"key":"2025030417540269900_r1","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.4049\/jimmunol.1800486","article-title":"Activation of NF-\u03baB in synovium versus cartilage from patients with advanced knee osteoarthritis: a potential contributor to inflammatory aspects of disease progression","volume":"201","author":"Ahmed","year":"2018","journal-title":"J. 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