{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T06:06:06Z","timestamp":1790921166892,"version":"4.1.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The Wiskott-Aldrich syndrome protein (WASp) is a key regulator of actin polimerization in hematopoietic cells. Mutations in WASp cause a severe immunodeficiency characterized by defective initiation of primary immune response and autoimmunity. The contribution of altered dendritic cells (DCs) functions to the disease pathogenesis has not been fully elucidated. In this study, we show that conventional DCs develop normally in WASp-deficient mice. However, Ag targeting to lymphoid organ-resident DCs via anti-DEC205 results in impaired naive CD8+ T cell activation, especially at low Ag doses. Altered trafficking of Ag-bearing DCs to lymph nodes (LNs) accounts only partially for defective priming because correction of DCs migration does not rescue T cell activation. In vitro and in vivo imaging of DC-T cell interactions in LNs showed that cytoskeletal alterations in WASp null DCs causes a reduction in the ability to form and stabilize conjugates with naive CD8+ T lymphocytes both in vitro and in vivo. These data indicate that WASp expression in DCs regulates both the ability to traffic to secondary lymphoid organs and to activate naive T cells in LNs.<\/jats:p>","DOI":"10.4049\/jimmunol.181.2.1135","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:27:05Z","timestamp":1397849225000},"page":"1135-1142","source":"Crossref","is-referenced-by-count":64,"title":["Expression of Wiskott-Aldrich Syndrome Protein in Dendritic Cells Regulates Synapse Formation and Activation of Naive CD8+ T Cells"],"prefix":"10.1093","volume":"181","author":[{"given":"Julian","family":"Pulecio","sequence":"first","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology , Trieste,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Tagliani","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology , Trieste,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alix","family":"Scholer","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche Medicale U653, Immunit\u00e9 et Cancer, Pavillon Pasteur, Institut Curie , Paris,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Prete","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology , Trieste,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luc","family":"Fetler","sequence":"additional","affiliation":[{"name":"Centre National de la Recherche Scientifique UMR 168, Laboratoire Physico-Chimie Curie, Institut Curie , Paris,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oscar R","family":"Burrone","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology , Trieste,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Benvenuti","sequence":"additional","affiliation":[{"name":"International Centre for Genetic Engineering and Biotechnology , Trieste,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,7,15]]},"reference":[{"key":"2026012807102401200_R1","first-page":"1685","article-title":"CD8+ but not CD8 \u2212 dendritic cells cross-prime cytotoxic T cells in vivo","volume-title":"J. 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