{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T17:12:16Z","timestamp":1793121136763,"version":"4.5.0"},"reference-count":73,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Shiga toxins (Stx) are the virulence factors of enterohemorrhagic Escherichia coli O157:H7, a worldwide emerging diarrheal pathogen, which precipitates postdiarrheal hemolytic uremic syndrome, the leading cause of acute renal failure in children. In this study, we show that Stx2 triggered expression of fractalkine (FKN), a CX3C transmembrane chemokine, acting as both adhesion counterreceptor on endothelial cells and soluble chemoattractant. Stx2 caused in HUVEC expression of FKN mRNA and protein, which promoted leukocyte capture, ablated by Abs to either endothelial FKN or leukocyte CX3CR1 receptor. Exposure of human glomerular endothelial cells to Stx2 recapitulated its FKN-inducing activity and FKN-mediated leukocyte adhesion. Both processes required phosphorylation of Src-family protein tyrosine kinase and p38 MAPK in endothelial cells. Furthermore, they depended on nuclear import of NF-\u03baB and other stress-responsive transcription factors. Inhibition of their nuclear import with the cell-penetrating SN50 peptide reduced FKN mRNA levels and FKN-mediated leukocyte capture by endothelial cells. Adenoviral overexpression of I\u03baB\u03b1 inhibited FKN mRNA up-regulation. The FKN-mediated responses to Stx2 were also dependent on AP-1. In mice, both virulence factors of Stx-producing E. coli, Stx and LPS, are required to elicit hemolytic uremic syndrome. In this study, FKN was detected within glomeruli of C57BL\/6 mice injected with Stx2, and further increased after Stx2 plus LPS coadministration. This was associated with recruitment of CX3CR1-positive cells. Thus, in response to Stx2, FKN is induced playing an essential role in the promotion of leukocyte-endothelial cell interaction thereby potentially contributing to the renal microvascular dysfunction and thrombotic microangiopathy that underlie hemolytic uremic syndrome due to enterohemorrhagic E. coli O157:H7 infection.<\/jats:p>","DOI":"10.4049\/jimmunol.181.2.1460","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:27:05Z","timestamp":1397849225000},"page":"1460-1469","source":"Crossref","is-referenced-by-count":37,"title":["Fractalkine and CX3CR1 Mediate Leukocyte Capture by Endothelium in Response to Shiga Toxin"],"prefix":"10.1093","volume":"181","author":[{"given":"Cristina","family":"Zanchi","sequence":"first","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla","family":"Zoja","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marina","family":"Morigi","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federica","family":"Valsecchi","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xue Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, Vanderbilt University , Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Rottoli","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica","family":"Locatelli","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simona","family":"Buelli","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Pezzotta","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Mapelli","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joyce","family":"Geelen","sequence":"additional","affiliation":[{"name":"Department of Pediatric Nephrology, Radboud University Nijmegen Medical Centre , Nijmegen,","place":["The Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Remuzzi","sequence":"additional","affiliation":[{"name":"Mario Negri Institute for Pharmacological Research , Bergamo,","place":["Italy"]},{"name":"Unit of Nephrology and Dialysis, Azienda Ospedaliera, Ospedali Riuniti di Bergamo ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacek","family":"Hawiger","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, School of Medicine, Vanderbilt University , Nashville, TN 37232"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,7,15]]},"reference":[{"key":"2026012807083877600_R1","first-page":"2","article-title":"The hemolytic uremic syndrome","volume-title":"Kidney Int.","year":"1995"},{"key":"2026012807083877600_R2","first-page":"S91","article-title":"Association of verotoxin-producing E. coli and verotoxin with hemolytic uremic syndrome","volume-title":"Kidney Int. 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