{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T16:02:10Z","timestamp":1773936130186,"version":"3.50.1"},"reference-count":74,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The interactions of the selectin family of adhesion molecules with their ligands are essential for the initial rolling stage of leukocyte trafficking. Under inflammatory conditions, the vascular selectins, E- and P-selectin, are expressed on activated vessels and interact with carbohydrate-based ligands on the leukocyte surface. While several ligands have been characterized on human T cells, monocytes and neutrophils, there is limited information concerning ligands on B cells. Endoglycan (EG) together with CD34 and podocalyxin comprise the CD34 family of sialomucins. We found that EG, previously implicated as an L-selectin ligand on endothelial cells, was present on human B cells, T cells and peripheral blood monocytes. Upon activation of B cells, EG increased with a concurrent decrease in PSGL-1. Expression of EG on T cells remained constant under the same conditions. We further found that native EG from several sources (a B cell line, a monocyte line and human tonsils) was reactive with HECA-452, a mAb that recognizes sialyl Lewis X and related structures. Moreover, immunopurified EG from these sources was able to bind to P-selectin and where tested E-selectin. This interaction was divalent cation-dependent and required sialylation of EG. Finally, an EG construct supported slow rolling of E- and P-selectin bearing cells in a sialic acid and fucose dependent manner, and the introduction of intact EG into a B cell line facilitated rolling interactions on a P-selectin substratum. These in vitro findings indicate that EG can function as a ligand for the vascular selectins.<\/jats:p>","DOI":"10.4049\/jimmunol.181.2.1480","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:27:05Z","timestamp":1397849225000},"page":"1480-1490","source":"Crossref","is-referenced-by-count":20,"title":["Endoglycan, a Member of the CD34 Family of Sialomucins, Is a Ligand for the Vascular Selectins"],"prefix":"10.1093","volume":"181","author":[{"given":"Sheena C","family":"Kerr","sequence":"first","affiliation":[{"name":"Department of Anatomy, University of California , San Francisco, CA 94143"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia B","family":"Fieger","sequence":"additional","affiliation":[{"name":"Department of Anatomy, University of California , San Francisco, CA 94143"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen R","family":"Snapp","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, University of Illinois , Chicago, IL 60612"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D","family":"Rosen","sequence":"additional","affiliation":[{"name":"Department of Anatomy, University of California , San Francisco, CA 94143"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,7,15]]},"reference":[{"key":"2026012807091452100_R1","first-page":"678","article-title":"Getting to the site of inflammation: the leukocyte adhesion cascade updated","volume-title":"Nat. Rev. Immunol.","year":"2007"},{"key":"2026012807091452100_R2","first-page":"190","article-title":"Direct observation of catch bonds involving cell-adhesion molecules","volume-title":"Nature","year":"2003"},{"key":"2026012807091452100_R3","first-page":"3259","article-title":"Selectins and their ligands: current concepts and controversies","volume-title":"Blood","year":"1996"},{"key":"2026012807091452100_R4","first-page":"60","article-title":"Lymphocyte homing and homeostasis","volume-title":"Science","year":"1996"},{"key":"2026012807091452100_R5","first-page":"19","article-title":"Glycosylation in the control of selectin counter-receptor structure and function","volume-title":"Immunol. Rev.","year":"2002"},{"key":"2026012807091452100_R6","first-page":"181","article-title":"Mechanisms that regulate the function of the selectins and their ligands","volume-title":"Physiol. Rev.","year":"1999"},{"key":"2026012807091452100_R7","first-page":"13841","article-title":"A distinct glycoform of CD44 is an L-selectin ligand on human hematopoietic cells","volume-title":"Proc. Natl. Acad. Sci. USA","year":"2000"},{"key":"2026012807091452100_R8","first-page":"26391","article-title":"Model glycosulfopeptides from P-selectin glycoprotein ligand-1 require tyrosine sulfation and a core 2-branched O-glycan to bind to L-selectin","volume-title":"J. Biol. Chem.","year":"2003"},{"key":"2026012807091452100_R9","first-page":"409","article-title":"Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment","volume-title":"Nat. Immunol.","year":"2007"},{"key":"2026012807091452100_R10","first-page":"129","article-title":"Ligands for L-selectin: homing, inflammation, and beyond","volume-title":"Annu. Rev. Immunol.","year":"2004"},{"key":"2026012807091452100_R11","first-page":"1096","article-title":"N-acetylglucosamine-6-O-sulfotransferases 1 and 2 cooperatively control lymphocyte homing through L-selectin ligand biosynthesis in high endothelial venules","volume-title":"Nat. Immunol.","year":"2005"},{"key":"2026012807091452100_R12","first-page":"1105","article-title":"A major class of L-selectin ligands is eliminated in mice deficient in two sulfotransferases expressed in high endothelial venules","volume-title":"Nat. Immunol.","year":"2005"},{"key":"2026012807091452100_R13","first-page":"2501","article-title":"Important contributions of P-selectin glycoprotein ligand-1-mediated secondary capture to human monocyte adhesion to P-selectin, E-selectin, and TNF-\u03b1-activated endothelium under flow in vitro","volume-title":"J. Immunol.","year":"1998"},{"key":"2026012807091452100_R14","first-page":"S97","article-title":"Role of PSGL-1 binding to selectins in leukocyte recruitment","volume-title":"J. Clin. Invest.","year":"1997"},{"key":"2026012807091452100_R15","first-page":"3010","article-title":"P-selectin glycoprotein ligand-1 is broadly expressed in cells of myeloid, lymphoid, and dendritic lineage and in some nonhematopoietic cells","volume-title":"Blood","year":"1996"},{"key":"2026012807091452100_R16","first-page":"523","article-title":"P-selectin glycoprotein ligand 1 is a ligand for L-selectin on neutrophils, monocytes, and CD34+ hematopoietic progenitor cells","volume-title":"J. Cell Biol.","year":"1996"},{"key":"2026012807091452100_R17","first-page":"154","article-title":"A novel P-selectin glycoprotein ligand-1 monoclonal antibody recognizes an epitope within the tyrosine sulfate motif of human PSGL-1 and blocks recognition of both P- and L-selectin","volume-title":"Blood","year":"1998"},{"key":"2026012807091452100_R18","first-page":"1023","article-title":"P-selectin glycoprotein ligand-1 is expressed on endothelial cells and mediates monocyte adhesion to activated endothelium","volume-title":"Arterioscler. Thromb. Vasc. Biol.","year":"2007"},{"key":"2026012807091452100_R19","first-page":"907","article-title":"Critical role of endothelial P-selectin glycoprotein ligand 1 in chronic murine ileitis","volume-title":"J. Exp. Med.","year":"2006"},{"key":"2026012807091452100_R20","first-page":"11662","article-title":"The P-selectin glycoprotein ligand functions as a common human leukocyte ligand for P- and E-selectins","volume-title":"J. Biol. Chem.","year":"1995"},{"key":"2026012807091452100_R21","first-page":"28786","article-title":"The binding of T cell-expressed P-selectin glycoprotein ligand-1 to E- and P-selectin is differentially regulated","volume-title":"J. Biol. Chem.","year":"1997"},{"key":"2026012807091452100_R22","first-page":"1669","article-title":"P-Selectin glycoprotein ligand 1 (PSGL-1) is a physiological ligand for E-selectin in mediating T helper 1 lymphocyte migration","volume-title":"J. Exp. Med.","year":"2000"},{"key":"2026012807091452100_R23","first-page":"661","article-title":"P-selectin glycoprotein ligand-1 mediates rolling of human neutrophils on P-selectin","volume-title":"J. Cell Biol.","year":"1995"},{"key":"2026012807091452100_R24","first-page":"1769","article-title":"Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectin-mediated neutrophil rolling and migration","volume-title":"J. Exp. Med.","year":"1999"},{"key":"2026012807091452100_R25","first-page":"939","article-title":"P-selectin glycoprotein ligand-1-deficient mice have impaired leukocyte tethering to E-selectin under flow","volume-title":"J. Clin. Invest.","year":"2002"},{"key":"2026012807091452100_R26","first-page":"1964","article-title":"The 130-kDa glycoform of CD43 functions as an E-selectin ligand for activated Th1 cells in vitro and in delayed-type hypersensitivity reactions in vivo","volume-title":"J. Invest. Dermatol.","year":"2007"},{"key":"2026012807091452100_R27","first-page":"1421","article-title":"CD43 is a ligand for E-selectin on CLA+ human T cells","volume-title":"Blood","year":"2006"},{"key":"2026012807091452100_R28","first-page":"8042","article-title":"CD43 functions as a ligand for E-Selectin on activated T cells","volume-title":"J. Immunol.","year":"2005"},{"key":"2026012807091452100_R29","first-page":"2499","article-title":"CD43 collaborates with P-selectin glycoprotein ligand-1 to mediate E-selectin-dependent T cell migration into inflamed skin","volume-title":"J. Immunol.","year":"2007"},{"key":"2026012807091452100_R30","first-page":"1183","article-title":"CD44 is a physiological E-selectin ligand on neutrophils","volume-title":"J. Exp. Med.","year":"2005"},{"key":"2026012807091452100_R31","first-page":"477","article-title":"Complete identification of E-selectin ligands on neutrophils reveals distinct functions of PSGL-1, ESL-1, and CD44","volume-title":"Immunity","year":"2007"},{"key":"2026012807091452100_R32","first-page":"37","article-title":"Molecular basis of leukocyte rolling on PSGL-1. Predominant role of core-2 O-glycans and of tyrosine sulfate residue 51","volume-title":"J. Biol. Chem.","year":"2003"},{"key":"2026012807091452100_R33","first-page":"2001","article-title":"Tyrosine sulfation enhances but is not required for PSGL-1 rolling adhesion on P-selectin","volume-title":"Biophys. J.","year":"2001"},{"key":"2026012807091452100_R34","first-page":"22677","article-title":"Tyrosine sulfation of P-selectin glycoprotein ligand-1 is required for high affinity binding to P-selectin","volume-title":"J. Biol. Chem.","year":"1995"},{"key":"2026012807091452100_R35","first-page":"3255","article-title":"Post-translational modifications of recombinant P-selectin glycoprotein ligand-1 required for binding to P- and E-selectin","volume-title":"J. Biol. Chem.","year":"1996"},{"key":"2026012807091452100_R36","first-page":"5378","article-title":"Regulation of PSGL-1 interactions with L-selectin, P-selectin, and E-selectin: role of human fucosyltransferase-IV and -VII","volume-title":"J. Biol. Chem.","year":"2005"},{"key":"2026012807091452100_R37","first-page":"978","article-title":"Cutaneous lymphocyte antigen is a specialized form of PSGL-1 expressed on skin-homing T cells","volume-title":"Nature","year":"1997"},{"key":"2026012807091452100_R38","first-page":"796","article-title":"ELAM-1 is an adhesion molecule for skin-homing T cells","volume-title":"Nature","year":"1991"},{"key":"2026012807091452100_R39","first-page":"1585","article-title":"B lymphocyte binding to E- and P-selectins is mediated through the de novo expression of carbohydrates on in vitro and in vivo activated human B cells","volume-title":"J. Clin. Invest.","year":"1994"},{"key":"2026012807091452100_R40","first-page":"807","article-title":"Expression of \u03b14\u03b27 and E-selectin ligand by circulating memory B cells: implications for targeted trafficking to mucosal and systemic sites","volume-title":"J. Leukocyte Biol.","year":"2000"},{"key":"2026012807091452100_R41","first-page":"2125","article-title":"An important role for the \u03b1 1,3 fucosyltransferase, FucT-VII, in leukocyte adhesion to E-selectin","volume-title":"Blood","year":"1996"},{"key":"2026012807091452100_R42","first-page":"1317","article-title":"Memory B lymphocytes from secondary lymphoid organs interact with E-selectin through a novel glycoprotein ligand","volume-title":"J. Clin. Invest.","year":"1999"},{"key":"2026012807091452100_R43","first-page":"78","article-title":"Tonsillar B cells do not express PSGL-1, but a significant fraction displays the cutaneous lymphocyte antigen and exhibits effective E- and P-selectin ligand activity","volume-title":"Int. Arch. Allergy Immunol.","year":"2001"},{"key":"2026012807091452100_R44","first-page":"1965","article-title":"Identification of podocalyxin-like protein as a high endothelial venule ligand for L-selectin: parallels to CD34","volume-title":"J. Exp. Med.","year":"1998"},{"key":"2026012807091452100_R45","first-page":"13","article-title":"Anuria, omphalocele, and perinatal lethality in mice lacking the CD34-related protein podocalyxin","volume-title":"J. Exp. Med.","year":"2001"},{"key":"2026012807091452100_R46","first-page":"13","article-title":"Beyond mere markers: functions for CD34 family of sialomucins in hematopoiesis","volume-title":"Immunol. Res.","year":"2006"},{"key":"2026012807091452100_R47","first-page":"9001","article-title":"Identification of endoglycan, a member of the CD34\/ podocalyxin family of sialomucins","volume-title":"J. Biol. Chem.","year":"2000"},{"key":"2026012807091452100_R48","first-page":"27390","article-title":"Endoglycan, a member of the CD34 family, functions as an L-selectin ligand through modification with tyrosine sulfation and sialyl lewis X","volume-title":"J. Biol. Chem.","year":"2003"},{"key":"2026012807091452100_R49","first-page":"2291","article-title":"Low force decelerates L-selectin dissociation from P-selectin glycoprotein ligand-1 and endoglycan","volume-title":"J. Biol. Chem.","year":"2004"},{"key":"2026012807091452100_R50","volume-title":"Using Antibodies","year":"1999"},{"key":"2026012807091452100_R51","first-page":"4494","article-title":"Attachment of the PSGL-1 cytoplasmic domain to the actin cytoskeleton is essential for leukocyte rolling on P-selectin","volume-title":"Blood","year":"2002"},{"key":"2026012807091452100_R52","first-page":"1632","article-title":"L-selectin can mediate leukocyte rolling in untreated mesenteric venules in vivo independent of E- or P-selectin","volume-title":"Blood","year":"1993"},{"key":"2026012807091452100_R53","first-page":"2764","article-title":"Activation of human leukocytes reduces surface P-selectin glycoprotein ligand-1 (PSGL-1, CD162) and adhesion to P-selectin in vitro","volume-title":"J. Immunol.","year":"2000"},{"key":"2026012807091452100_R54","first-page":"239","article-title":"Memory B cells from human tonsils colonize mucosal epithelium and directly present antigen to T cells by rapid up-regulation of B7-1 and B7-2","volume-title":"Immunity","year":"1995"},{"key":"2026012807091452100_R55","first-page":"546","article-title":"Sulfated sialyl Lewis X, the putative L-selectin ligand, detected on endothelial cells of high endothelial venules by a distinct set of anti-sialyl Lewis X antibodies","volume-title":"Biochem. Biophys. Res. Commun.","year":"1997"},{"key":"2026012807091452100_R56","first-page":"5070","article-title":"L-selectin ligands expressed by human leukocytes are HECA-452 antibody-defined carbohydrate epitopes preferentially displayed by P-selectin glycoprotein ligand-1","volume-title":"J. Immunol.","year":"1999"},{"key":"2026012807091452100_R57","first-page":"602","article-title":"Glycosylation-dependent inhibition of cutaneous lymphocyte-associated antigen expression: implications in modulating lymphocyte migration to skin","volume-title":"Blood","year":"2003"},{"key":"2026012807091452100_R58","first-page":"3197","article-title":"Identification of cutaneous lymphocyte-associated antigen as sialyl 6-sulfo Lewis X, a selectin ligand expressed on a subset of skin-homing helper memory T cells","volume-title":"Blood","year":"2006"},{"key":"2026012807091452100_R59","first-page":"543","article-title":"Glycosylation might provide endothelial zip codes for organ-specific leukocyte traffic into inflammatory sites","volume-title":"Am. J. Pathol.","year":"2002"},{"key":"2026012807091452100_R60","first-page":"2005","article-title":"CD34 facilitates the development of allergic asthma","volume-title":"Blood","year":"2007"},{"key":"2026012807091452100_R61","first-page":"1211","article-title":"CD34 is a specific marker of mature murine mast cells","volume-title":"Exp. Hematol.","year":"2002"},{"key":"2026012807091452100_R62","first-page":"43","article-title":"CD34 and CD43 inhibit mast cell adhesion and are required for optimal mast cell reconstitution","volume-title":"Immunity","year":"2005"},{"key":"2026012807091452100_R63","first-page":"5068","article-title":"Overexpression of the anti-adhesin podocalyxin is an independent predictor of breast cancer progression","volume-title":"Cancer Res.","year":"2004"},{"key":"2026012807091452100_R64","first-page":"436","article-title":"Binding of L-selectin to the vascular sialomucin CD34","volume-title":"Science","year":"1993"},{"key":"2026012807091452100_R65","first-page":"261","article-title":"Sialomucin CD34 is the major L-selectin ligand in human tonsil high endothelial venules","volume-title":"J. Cell Biol.","year":"1995"},{"key":"2026012807091452100_R66","first-page":"3247","article-title":"Differential expression of homing-associated adhesion molecules by T cell subsets in man","volume-title":"J. Immunol.","year":"1990"},{"key":"2026012807091452100_R67","first-page":"577","article-title":"Neutrophils, monocytes, and dendritic cells express the same specialized form of PSGL-1 as do skin-homing memory T cells: cutaneous lymphocyte antigen","volume-title":"Biochem. Biophys. Res. Commun.","year":"2001"},{"key":"2026012807091452100_R68","first-page":"127","article-title":"Chemokines, sphingosine-1-phosphate, and cell migration in secondary lymphoid organs","volume-title":"Annu. Rev. Immunol.","year":"2005"},{"key":"2026012807091452100_R69","first-page":"2905","article-title":"IgG plasma cells display a unique spectrum of leukocyte adhesion and homing molecules","volume-title":"Blood","year":"2002"},{"key":"2026012807091452100_R70","first-page":"1355","article-title":"P-selectin glycoprotein ligand-1 mediates L-selectin-dependent leukocyte rolling in venules","volume-title":"J. Exp. Med.","year":"2003"},{"key":"2026012807091452100_R71","first-page":"532","article-title":"Interaction of the selectin ligand PSGL-1 with chemokines CCL21 and CCL19 facilitates efficient homing of T cells to secondary lymphoid organs","volume-title":"Nat. Immunol.","year":"2007"},{"key":"2026012807091452100_R72","first-page":"51775","article-title":"Human P-selectin glycoprotein ligand-1 (PSGL-1) interacts with the skin-associated chemokine CCL27 via sulfated tyrosines at the PSGL-1 amino terminus","volume-title":"J. Biol. Chem.","year":"2004"},{"key":"2026012807091452100_R73","first-page":"5228","article-title":"Versican interacts with chemokines and modulates cellular responses","volume-title":"J. Biol. Chem.","year":"2001"},{"key":"2026012807091452100_R74","first-page":"12921","article-title":"Oversulfated chondriotin\/ dermatin sulfates containing GlcA\u03b21\/Ido 1\u20133\u03b1GalNAc (4\u20136-O-disulfate) interact with L- and P-Selectin and chemokines","volume-title":"J. Biol. Chem.","year":"2002"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/181\/2\/1480\/62583282\/7415.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/181\/2\/1480\/62583282\/7415.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T12:10:34Z","timestamp":1769602234000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/181\/2\/1480\/8008080"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,7]]},"references-count":74,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2008,7,15]]},"published-print":{"date-parts":[[2008,7,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.181.2.1480","relation":{},"ISSN":["1550-6606","0022-1767"],"issn-type":[{"value":"1550-6606","type":"electronic"},{"value":"0022-1767","type":"print"}],"subject":[],"published-other":{"date-parts":[[2008,7]]},"published":{"date-parts":[[2008,7]]}}}