{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T23:21:54Z","timestamp":1774394514983,"version":"3.50.1"},"reference-count":54,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2008,9,1]],"date-time":"2008-09-01T00:00:00Z","timestamp":1220227200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Porphyromonas gingivalis is a Gram-negative bacterium strongly associated with chronic periodontitis, an inflammatory oral disease. A major virulence factor common to all characterized strains of P. gingivalis is the RgpA-Kgp proteinase-adhesin complexes (RgpA-Kgp complexes). In this study, we investigated T cell proliferative and cytokine responses to the RgpA-Kgp complexes and identified T cell epitopes in BALB\/c mice utilizing Pepscan methodology. T cell proliferative responses were found to be predominantly directed toward the proteinase catalytic domains. Eleven T cell epitopes were identified using RgpA-Kgp-primed lymph node T cells (IL-4 dominant) and 21 using an RgpA-Kgp-specific T cell line (IFN-\u03b3 dominant), with 5 T cell epitopes, including the immunodominant epitope peptide 22, common to both T cell populations. Peptide 22 (439ANYTAHGSETAWADP453) from the Kgp proteinase catalytic domain induced a Th2 cytokine response in mice, and peptide 22-primed T cells had a Th2 cytokine profile when stimulated with the RgpA-Kgp complexes. Truncation and alanine scanning of peptide 22 identified the minimum epitope (442TAHGSETAWA451), and residues His444, Glu447, and Trp450 as critical for T cell proliferation. With a view to vaccine development, peptide 22 was incorporated into a synthetic peptide polymer. Peptide 22 polymer induced strong T cell proliferation and crossreactivity to native RgpA-Kgp complexes. In conclusion, we have identified a major T cell epitope of P. gingivalis and established that antigenicity of the T cell epitope is retained when delivered as a peptide polymer. The strategies employed here may have potential in the development of a synthetic peptide vaccine for P. gingivalis.<\/jats:p>","DOI":"10.4049\/jimmunol.181.6.4150","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:24:33Z","timestamp":1397849073000},"page":"4150-4158","source":"Crossref","is-referenced-by-count":9,"title":["Characterization of T Cell Responses to the RgpA-Kgp Proteinase-Adhesin Complexes of\n                    <i>Porphyromonas gingivalis<\/i>\n                    in BALB\/c Mice"],"prefix":"10.1093","volume":"181","author":[{"given":"Vivian","family":"Tam","sequence":"first","affiliation":[{"name":"Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne , Victoria,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neil M","family":"O'Brien-Simpson","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne , Victoria,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rishi D","family":"Pathirana","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne , Victoria,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leanne T","family":"Frazer","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne , Victoria,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric C","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Cooperative Research Centre for Oral Health Science, School of Dental Science, The University of Melbourne , Victoria,","place":["Australia"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2008,9,15]]},"reference":[{"key":"2026012807090874300_R1","first-page":"269","article-title":"Periodontal diseases in the United States population","volume-title":"J. 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