{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:07:47Z","timestamp":1791036467789,"version":"4.1.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2008,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>At the temperature of its flea vector (\u223c20\u201330\u00b0C), the causative agent of plague, Yersinia pestis, expresses a profile of genes distinct from those expressed in a mammalian host (37\u00b0C). When dendritic cells (DC) are exposed to Y. pestis grown at 26\u00b0C (Y. pestis-26\u00b0), they secrete copious amounts of IL-12p40 homodimer (IL-12(p40)2). In contrast, when DCs are exposed to Y. pestis grown at 37\u00b0C (Y. pestis-37\u00b0), they transcribe very little IL-12p40, which is secreted as IL-12p40 monomer (IL-12p40). Y. pestis-26\u00b0 also induces migration of DCs to the homeostatic chemokine CCL19, whereas Y. pestis-37\u00b0 does not; migratory DCs are positive for IL-12p40 transcription and secrete mostly IL-12(p40)2; DCs lacking IL-12p40 do not migrate. Expression of acyltransferase LpxL from Escherichia coli in Y. pestis-37\u00b0 results in the production of a hexa-acylated lipid A, also seen in Y. pestis-26\u00b0, rather than tetra-acylated lipid A normally seen in Y. pestis-37\u00b0. The LpxL-expressing Y. pestis-37\u00b0 promotes DC IL-12(p40)2 production and induction of DC migration. In addition, absence of TLR4 ablates production of IL-12(p40)2 in DC exposed to Y. pestis-26\u00b0. The data demonstrate the molecular pathway by which Y. pestis evades induction of early DC activation as measured by migration and IL-12(p40)2 production.<\/jats:p>","DOI":"10.4049\/jimmunol.181.8.5560","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:47:01Z","timestamp":1397850421000},"page":"5560-5567","source":"Crossref","is-referenced-by-count":37,"title":["<i>Yersinia pestis<\/i>\n                    Evades TLR4-dependent Induction of IL-12(p40)2 by Dendritic Cells and Subsequent Cell Migration"],"prefix":"10.1093","volume":"181","author":[{"given":"Richard T","family":"Robinson","sequence":"first","affiliation":[{"name":"Trudeau Institute , Saranac Lake, NY 12983"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shabaana A","family":"Khader","sequence":"additional","affiliation":[{"name":"Trudeau Institute , Saranac Lake, NY 12983"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard M","family":"Locksley","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Department of Medicine and Microbiology and Immunology, University of California , San Francisco, CA 94143"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egil","family":"Lien","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases and Immunology, Department of Medicine, University of Massachusetts Medical School , Worcester, MA 01655"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen T","family":"Smiley","sequence":"additional","affiliation":[{"name":"Trudeau Institute , Saranac Lake, NY 12983"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea M","family":"Cooper","sequence":"additional","affiliation":[{"name":"Trudeau Institute , Saranac Lake, NY 12983"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2008,10,15]]},"reference":[{"key":"2026012807142310600_R1","first-page":"35","article-title":"Yersinia pestis: etiologic agent of plague","volume-title":"Clin. 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