{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T02:48:52Z","timestamp":1790045332797,"version":"4.0.1"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2009,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>LFA-1 is critical for NK cell cytotoxicity because it mediates adhesion of NK cells to target cells. Talin is thought to associate with the cytoplasmic tail of LFA-1 and activates its ligand-binding function. In this study, we report that talin is also required for LFA-1-mediated outside-in signaling leading to NK cell polarization. NK cells generated from talin1-deficient murine embryonic stem cells are defective in LFA-1-mediated adhesion. Although exogenously added manganese activates LFA-1 on talin-deficient NK cells and induces conjugate formation with target cells, their LFA-1-dependent cytotoxicity is impaired. Binding of ICAM-1-coated beads to wild-type NK cells induces reorganization of the actin cytoskeleton and coligation of the activating receptor NKG2D induces polarization of cytotoxic granules, whereas talin1-deficient NK cells fail to polarize with or without NKG2D coligation. Thus, talin1 plays a dual role in NK cell cytotoxicity, first by activation of LFA-1-mediated adhesion and then via LFA-1-induced NK cell polarization.<\/jats:p>","DOI":"10.4049\/jimmunol.182.2.948","type":"journal-article","created":{"date-parts":[[2014,4,18]],"date-time":"2014-04-18T19:00:37Z","timestamp":1397847637000},"page":"948-956","source":"Crossref","is-referenced-by-count":66,"title":["A Dual Role for Talin in NK Cell Cytotoxicity: Activation of LFA-1-Mediated Cell Adhesion and Polarization of NK Cells"],"prefix":"10.1093","volume":"182","author":[{"given":"Emily M","family":"Mace","sequence":"first","affiliation":[{"name":"Terry Fox Laboratory, British Columbia Cancer Agency , Vancouver, British Columbia,","place":["Canada"]},{"name":"Genetics Graduate Program"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan J","family":"Monkley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Leicester , Leicester,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David R","family":"Critchley","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Leicester , Leicester,","place":["United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumio","family":"Takei","sequence":"additional","affiliation":[{"name":"Terry Fox Laboratory, British Columbia Cancer Agency , Vancouver, British Columbia,","place":["Canada"]},{"name":"Department of Pathology and Laboratory Medicine, University of British Columbia , Vancouver, British Columbia,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2009,1,15]]},"reference":[{"key":"2026012806211466500_R1","first-page":"5781","article-title":"Cooperation between CD44 and LFA-1\/CD11a adhesion receptors in lymphokine-activated killer cell cytotoxicity.","volume-title":"J. 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