{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:15:05Z","timestamp":1787724905924,"version":"build-2784847793"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Gasdermin D (GSDMD) cleavage by caspase-1 or caspase-11 inflammasomes triggers pyroptosis, a lytic form of cell death protective against intracellular bacteria. In this study, we examine the role of GSDMD in a mouse model of melioidosis. Gsdmd\u2212\/\u2212 mice were more susceptible than wild-type mice to intranasal infection with Burkholderia thailandensis. Production of IL-18, but not IL-1\u03b2, was decreased in Gsdmd\u2212\/\u2212 infected mice. Despite lower IL-18, IFN-\u03b3 was produced in similar amounts in wild-type and Gsdmd\u2212\/\u2212 mice. In vitro, secretion of both IL-1\u03b2 and IL-18 by macrophages or dendritic cells infected with B. thailandensis was dependent on GSDMD. Surprisingly, wild-type or GSDMD-deficient neutrophils secreted similar amounts of IL-1\u03b2, suggesting these cells may be the source of the GSDMD-independent IL-1\u03b2 detected in vivo. Recombinant GSDMD was able to directly kill B. thailandensis in vitro upon processing by active caspase-1. Moreover, bacteria harvested from wild-type, but not Gsdmd\u2212\/\u2212, macrophages were more susceptible to the microbicidal effect of hydrogen peroxide or human \u03b2-defensin-3. Finally, we provide evidence that pyroptosis of in vitro infected macrophages is directly microbicidal. Taken together, these results indicate that the protective action of GSDMD in melioidosis is primarily due to induction of pyroptosis and direct killing of bacteria rather than production of cytokines.<\/jats:p>","DOI":"10.4049\/jimmunol.1900045","type":"journal-article","created":{"date-parts":[[2019,4,29]],"date-time":"2019-04-29T16:50:50Z","timestamp":1556556650000},"page":"3468-3473","source":"Crossref","is-referenced-by-count":75,"title":["Gasdermin D Protects from Melioidosis through Pyroptosis and Direct Killing of Bacteria"],"prefix":"10.1093","volume":"202","author":[{"given":"Jinyong","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, IL 60064"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7569-4586","authenticated-orcid":false,"given":"Kelly","family":"Deobald","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, IL 60064"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1181-0717","authenticated-orcid":false,"given":"Fabio","family":"Re","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Rosalind Franklin University of Medicine and Science , North Chicago, IL 60064"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,6,15]]},"reference":[{"key":"2025030417562479200_r1","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1038\/ni.1960","article-title":"Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria","volume":"11","author":"Miao","year":"2010","journal-title":"Nat. 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