{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,5]],"date-time":"2025-03-05T05:46:53Z","timestamp":1741153613805,"version":"3.38.0"},"reference-count":45,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2019,7,1]],"date-time":"2019-07-01T00:00:00Z","timestamp":1561939200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mucosal plasma cells (PC) and Ig production are essential to fend pathogens and to maintain mucosal homeostasis. In human Helicobacter pylori infection, mucosal PC express inducible NO synthase (iNOS), which positively correlates with clearance of experimental human infection. To characterize Ig genes and specificities of antral mucosal iNOS+ and iNOS\u2212 PC in H. pylori infection, we sequenced rearranged Ig genes from single cell\u2013sorted PC from biopsy specimens of chronically infected patients and analyzed them with respect to their molecular features. The binding specificity of individual PC\u2019s Ig was determined following recombinant expression. We identified high rates of somatic hypermutations, especially targeting RGYW\/WRCY hotspot motifs in the individual Ig genes, indicating T cell\u2013dependent maturation. For seven of 14 recombinantly expressed Ig, Ag specificity could be determined. Two clones reacted to H. pylori proteins, and five were found to be polyreactive against LPSs, dsDNA, and ssDNA. All specific Ig originated from iNOS+ PC. H. pylori\u2013specific Ig are encoded by V and J family genes previously shown to be also used in rearranged Ig loci of MALT B cell lymphomas. In summary, mucosal iNOS+ PC producing H. pylori\u2013specific Ig accumulate in infection and appear to be a product of T cell\u2013dependent B cell maturation. Moreover, the Ig\u2019s molecular features partly resembled that of MALT B cell lymphoma Ig genes, suggestive of a mechanism in which a progressive molecular evolution of pathogen-specific B cells to MALT B cell lymphoma occurs.<\/jats:p>","DOI":"10.4049\/jimmunol.1900074","type":"journal-article","created":{"date-parts":[[2019,5,17]],"date-time":"2019-05-17T20:05:39Z","timestamp":1558123539000},"page":"208-215","source":"Crossref","is-referenced-by-count":0,"title":["T Cell\u2013Dependent Maturation of Pathogen-Specific Igs in the Antrum of Chronically <i>Helicobacter pylori<\/i>\u2013Infected Patients"],"prefix":"10.1093","volume":"203","author":[{"given":"Laura","family":"Neumann","sequence":"first","affiliation":[{"name":"Medizinische Klinik f\u00fcr Gastroenterologie, Infektiologie und Rheumatologie, Charit\u00e9 - Universit\u00e4tsmedizin Berlin , 12203 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9558-7291","authenticated-orcid":false,"given":"Verena","family":"Moos","sequence":"additional","affiliation":[{"name":"Medizinische Klinik f\u00fcr Gastroenterologie, Infektiologie und Rheumatologie, Charit\u00e9 - Universit\u00e4tsmedizin Berlin , 12203 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5927-7018","authenticated-orcid":false,"given":"Claudia","family":"Giesecke-Thiel","sequence":"additional","affiliation":[{"name":"Abteilung f\u00fcr Medizin, Rheumatologie und Klinische Immunology, Charit\u00e9 - Universit\u00e4tsmedizin Berlin , 10117 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"D\u00f6rner","sequence":"additional","affiliation":[{"name":"Abteilung f\u00fcr Medizin, Rheumatologie und Klinische Immunology, Charit\u00e9 - Universit\u00e4tsmedizin Berlin , 10117 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Ignatius","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Tropenmedizin und Internationale Gesundheit, Charit\u00e9 - Universit\u00e4tsmedizin Berlin , 13353 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristina","family":"Allers","sequence":"additional","affiliation":[{"name":"Medizinische Klinik f\u00fcr Gastroenterologie, Infektiologie und Rheumatologie, Charit\u00e9 - Universit\u00e4tsmedizin Berlin , 12203 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4875-8457","authenticated-orcid":false,"given":"Toni","family":"Aebischer","sequence":"additional","affiliation":[{"name":"Robert Koch Institute , 13353 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Schneider","sequence":"additional","affiliation":[{"name":"Medizinische Klinik f\u00fcr Gastroenterologie, Infektiologie und Rheumatologie, Charit\u00e9 - Universit\u00e4tsmedizin Berlin , 12203 Berlin ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,7,1]]},"reference":[{"key":"2025030418133137900_r1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/j.0105-2896.2005.00283.x","article-title":"Mucosal B cells: phenotypic characteristics, transcriptional regulation, and homing properties","volume":"206","author":"Brandtzaeg","year":"2005","journal-title":"Immunol. 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