{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:27:46Z","timestamp":1787030866799,"version":"build-2736575974"},"reference-count":50,"publisher":"Oxford University Press (OUP)","issue":"12","license":[{"start":{"date-parts":[[2019,6,1]],"date-time":"2019-06-01T00:00:00Z","timestamp":1559347200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>PGE2 is a lipid-signaling molecule with complex roles in both homeostasis and inflammation. Depending on the cellular context, PGE2 may also suppress certain immune responses. In this study, we tested whether PGE2 could inhibit bacterial killing by polymorphonuclear neutrophils (PMN) using a mouse model of foodborne listeriosis. We found that PGE2 pretreatment decreased the ability of PMN harvested from the bone marrow of either BALB\/cByJ or C57BL\/6J mice to kill Listeria monocytogenes in vitro. PGE2 treatment slowed the migration of PMN toward the chemoattractant leukotriene B4, decreased uptake of L. monocytogenes by PMN, and inhibited the respiratory burst of PMN compared with vehicle-treated cells. When immune cells were isolated from the livers of infected mice and tested directly ex vivo for the presence of PGE2, BALB\/cByJ cells produced significantly more than C57BL\/6J cells. Together, these data suggest that robust PGE2 production can suppress PMN effector functions, leading to decreased bacterial killing, which may contribute to the innate susceptibility of BALB\/cByJ mice to infection with the facultative intracellular bacterial pathogen L. monocytogenes.<\/jats:p>","DOI":"10.4049\/jimmunol.1900201","type":"journal-article","created":{"date-parts":[[2019,5,7]],"date-time":"2019-05-07T13:07:36Z","timestamp":1557234456000},"page":"3474-3482","source":"Crossref","is-referenced-by-count":16,"title":["Prostaglandin E2 Inhibits the Ability of Neutrophils to Kill\n                    <i>Listeria monocytogenes<\/i>"],"prefix":"10.1093","volume":"202","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6698-3199","authenticated-orcid":false,"given":"Michelle G","family":"Pitts","sequence":"first","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0716-3518","authenticated-orcid":false,"given":"Sarah E F","family":"D\u2019Orazio","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Immunology, and Molecular Genetics, University of Kentucky College of Medicine , Lexington, KY 40536"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2019,6,15]]},"reference":[{"key":"2025030417562533400_r1","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1016\/j.pharmthera.2017.12.008","article-title":"Prostaglandin E2 as a regulator of immunity to pathogens","volume":"185","author":"Mart\u00ednez-Col\u00f3n","year":"2018","journal-title":"Pharmacol. 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Med."},{"key":"2025030417562533400_r13","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1111\/j.1749-6632.1994.tb52729.x","article-title":"Prostanoid receptors. Structure, function, and distribution","volume":"744","author":"Narumiya","year":"1994","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2025030417562533400_r14","doi-asserted-by":"crossref","DOI":"10.1155\/2013\/683405","article-title":"The immunobiology of prostanoid receptor signaling in connecting innate and adaptive immunity","volume":"2013","author":"Harizi","year":"2013","journal-title":"BioMed Res. Int."},{"key":"2025030417562533400_r15","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.pharmthera.2004.06.003","article-title":"Pharmacology and signaling of prostaglandin receptors: multiple roles in inflammation and immune modulation","volume":"103","author":"Hata","year":"2004","journal-title":"Pharmacol. 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Immunol."},{"key":"2025030417562533400_r23","doi-asserted-by":"crossref","DOI":"10.1371\/journal.ppat.1003015","article-title":"InlA promotes dissemination of Listeria monocytogenes to the mesenteric lymph nodes during food borne infection of mice","volume":"8","author":"Bou Ghanem","year":"2012","journal-title":"PLoS Pathog."},{"key":"2025030417562533400_r24","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.1128\/IAI.00503-15","article-title":"Intracellular Listeria monocytogenes comprises a minimal but vital fraction of the intestinal burden following foodborne infection","volume":"83","author":"Jones","year":"2015","journal-title":"Infect. Immun."},{"key":"2025030417562533400_r25","doi-asserted-by":"crossref","DOI":"10.1128\/IAI.00085-18","article-title":"Neutrophils from both susceptible and resistant mice efficiently kill opsonized Listeria monocytogenes","volume":"86","author":"Pitts","year":"2018","journal-title":"Infect. 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Chem."},{"key":"2025030417562533400_r35","doi-asserted-by":"crossref","first-page":"724","DOI":"10.1111\/j.2042-7158.1983.tb02878.x","article-title":"Elimination from the circulation of cats of 6-keto-prostaglandin E1 compared with prostaglandins E2 and I2","volume":"35","author":"F\u00f6rstermann","year":"1983","journal-title":"J. Pharm. Pharmacol."},{"key":"2025030417562533400_r36","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1016\/j.devcel.2012.02.003","article-title":"LTB4 is a signal-relay molecule during neutrophil chemotaxis","volume":"22","author":"Afonso","year":"2012","journal-title":"Dev. 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Cell Biol."},{"key":"2025030417562533400_r40","doi-asserted-by":"crossref","first-page":"1031","DOI":"10.1016\/S0026-895X(25)09407-6","article-title":"Two Gs-coupled prostaglandin E receptor subtypes, EP2 and EP4, differ in desensitization and sensitivity to the metabolic inactivation of the agonist","volume":"50","author":"Nishigaki","year":"1996","journal-title":"Mol. Pharmacol."},{"key":"2025030417562533400_r41","doi-asserted-by":"crossref","first-page":"1492","DOI":"10.1128\/IAI.01207-08","article-title":"Multiple mechanisms contribute to the robust rapid gamma interferon response by CD8+ T cells during Listeria monocytogenes infection","volume":"77","author":"Bou Ghanem","year":"2009","journal-title":"Infect. Immun."},{"key":"2025030417562533400_r42","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/85812","article-title":"Multigenic control of Listeria monocytogenes susceptibility in mice","volume":"27","author":"Boyartchuk","year":"2001","journal-title":"Nat. Genet."},{"key":"2025030417562533400_r43","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.4049\/jimmunol.132.4.2078","article-title":"Genetic control of resistance to Listeria monocytogenes: regulation of leukocyte inflammatory responses by the Hc locus","volume":"132","author":"Gervais","year":"1984","journal-title":"J. Immunol."},{"key":"2025030417562533400_r44","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1006\/cimm.2000.1644","article-title":"Differences in interleukin-12 and -15 production by dendritic cells at the early stage of Listeria monocytogenes infection between BALB\/c and C57 BL\/6 mice","volume":"202","author":"Liu","year":"2000","journal-title":"Cell. 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Immunol."},{"key":"2025030417562533400_r48","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1046\/j.1365-2567.2002.01403.x","article-title":"The role of prostaglandin E2 in the immunopathogenesis of experimental pulmonary tuberculosis","volume":"106","author":"Rangel Moreno","year":"2002","journal-title":"Immunology"},{"key":"2025030417562533400_r49","doi-asserted-by":"crossref","first-page":"3915","DOI":"10.4049\/jimmunol.137.12.3915","article-title":"The functional significance of the regulation of macrophage Ia expression by endogenous arachidonate metabolites in vitro","volume":"137","author":"Tripp","year":"1986","journal-title":"J. Immunol."},{"key":"2025030417562533400_r50","doi-asserted-by":"crossref","first-page":"612","DOI":"10.1038\/89759","article-title":"Lipid mediator class switching during acute inflammation: signals in resolution","volume":"2","author":"Levy","year":"2001","journal-title":"Nat. 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