{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T22:03:39Z","timestamp":1768687419473,"version":"3.49.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2019,9,1]],"date-time":"2019-09-01T00:00:00Z","timestamp":1567296000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2019,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Surfactant protein-A (SP-A) is an important mediator of pulmonary immunity. A specific genetic variation in SP-A2, corresponding to a glutamine (Q) to lysine (K) amino acid substitution at position 223 of the lectin domain, was shown to alter the ability of SP-A to inhibit eosinophil degranulation. Because a large subgroup of asthmatics have associated eosinophilia, often accompanied by inflammation associated with delayed clearance, our goal was to define how SP-A mediates eosinophil resolution in allergic airways and whether genetic variation affects this activity. Wild-type, SP-A knockout (SP-A KO) and humanized (SP-A2 223Q\/Q, SP-A2 223K\/K) C57BL\/6 mice were challenged in an allergic OVA model, and parameters of inflammation were examined. Peripheral blood eosinophils were isolated to assess the effect of SP-A genetic variation on apoptosis and chemotaxis. Five days postchallenge, SP-A KO and humanized SP-A2 223K\/K mice had persistent eosinophilia in bronchoalveolar lavage fluid compared with wild-type and SP-A2 223Q\/Q mice, suggesting an impairment in eosinophil resolution. In vitro, human SP-A containing either the 223Q or the 223K allele was chemoattractant for eosinophils whereas only 223Q resulted in decreased eosinophil viability. Our results suggest that SP-A aids in the resolution of allergic airway inflammation by promoting eosinophil clearance from lung tissue through chemotaxis, independent of SP-A2 Q223K, and by inducing apoptosis of eosinophils, which is altered by the polymorphism.<\/jats:p>","DOI":"10.4049\/jimmunol.1900546","type":"journal-article","created":{"date-parts":[[2019,7,26]],"date-time":"2019-07-26T20:35:14Z","timestamp":1564173314000},"page":"1122-1130","source":"Crossref","is-referenced-by-count":13,"title":["Genetic Variation in Surfactant Protein-A2 Delays Resolution of Eosinophilia in Asthma"],"prefix":"10.1093","volume":"203","author":[{"given":"Alane Blythe C","family":"Dy","sequence":"first","affiliation":[{"name":"Clinical Translational Sciences, University of Arizona Health Sciences , Tucson, AZ 85721"},{"name":"Asthma and Airway Disease Research Center , Tucson, AZ 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Muhammad Z","family":"Arif","sequence":"additional","affiliation":[{"name":"Department of Medicine, University of Arizona , Tucson, AZ 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth J","family":"Addison","sequence":"additional","affiliation":[{"name":"Asthma and Airway Disease Research Center , Tucson, AZ 85724"},{"name":"Department of Cellular and Molecular Medicine, University of Arizona , Tucson, AZ 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9188-9244","authenticated-orcid":false,"given":"Loretta G","family":"Que","sequence":"additional","affiliation":[{"name":"Department of Medicine, Duke University School of Medicine , Durham, NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Boitano","sequence":"additional","affiliation":[{"name":"Asthma and Airway Disease Research Center , Tucson, AZ 85724"},{"name":"Department of Physiology, University of Arizona , Tucson, AZ 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monica","family":"Kraft","sequence":"additional","affiliation":[{"name":"Asthma and Airway Disease Research Center , Tucson, AZ 85724"},{"name":"Department of Medicine, University of Arizona , Tucson, AZ 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julie G","family":"Ledford","sequence":"additional","affiliation":[{"name":"Asthma and Airway Disease Research Center , Tucson, AZ 85724"},{"name":"Department of Cellular and Molecular Medicine, University of Arizona , Tucson, AZ 85724"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2019,9,1]]},"reference":[{"key":"2025030417541578900_r1","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1038\/nri1528","article-title":"Immunoregulatory functions of surfactant proteins","volume":"5","author":"Wright","year":"2005","journal-title":"Nat. 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