{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T11:10:00Z","timestamp":1779189000105,"version":"3.51.4"},"reference-count":41,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2020,6,1]],"date-time":"2020-06-01T00:00:00Z","timestamp":1590969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["RTG 1949"],"award-info":[{"award-number":["RTG 1949"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009400","name":"Medical Faculty of the Heinrich Heine University D\u00fcsseldorf","doi-asserted-by":"publisher","award":["FoKo2018-44"],"award-info":[{"award-number":["FoKo2018-44"]}],"id":[{"id":"10.13039\/501100009400","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Infection with the human CMV associates with phenotypic alterations in lymphocyte subsets. A highly reproducible finding in CMV-seropositive individuals is an expansion of NKG2Cpos NK cells. In this study, we analyzed if the altered NK cell compartment in CMV-seropositive human donors may affect CMV-specific CD8 T cells. Resting CMV-specific CD8 T cells were terminally differentiated and expressed high levels of the NKG2C ligand HLA-E. Activation of CMV-specific CD8 T cells with the cognate Ag further increased HLA-E expression. In line with a negative regulatory effect of NKG2Cpos NK cells on HLA-Ehigh CD8 T cells, depletion of NKG2Cpos NK cells enhanced Ag-specific expansion of CMV-specific CD8 T cells in vitro. In turn, the activation of NK cells in coculture with CMV-specific CD8 T cells promoted a selective loss of HLA-Ehigh CD8 T cells. To test if NKG2Cpos NK cells can target HLA-Ehigh CD8 T cells, Jurkat T cells with and without stabilized HLA-E on the surface were used. NKG2Cpos NK cells stimulated with HLA-Ehigh Jurkat cells released higher levels of Granzyme B compared with NKG2Cneg NK cells and NKG2Cpos NK cells stimulated with HLA-Elow Jurkat cells. Moreover, intracellular levels of caspase 3\/7 were increased in HLA-Ehigh Jurkat cells compared with HLA-Elow Jurkat cells, consistent with higher rates of apoptosis in HLA-Ehigh T cells in the presence of NKG2Cpos NK cells. Our data show that NKG2Cpos NK cells interact with HLA-Ehigh CD8 T cells, which may negatively regulate the expansion of CMV-specific CD8 T cells upon activation.<\/jats:p>","DOI":"10.4049\/jimmunol.1901281","type":"journal-article","created":{"date-parts":[[2020,4,13]],"date-time":"2020-04-13T15:44:27Z","timestamp":1586792667000},"page":"2910-2917","source":"Crossref","is-referenced-by-count":13,"title":["NKG2Cpos NK Cells Regulate the Expansion of Cytomegalovirus-Specific CD8 T Cells"],"prefix":"10.1093","volume":"204","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0861-8262","authenticated-orcid":false,"given":"Ralf","family":"Grutza","sequence":"first","affiliation":[{"name":"Institute of Virology, University Hospital D\u00fcsseldorf, Heinrich Heine University, Medical Faculty , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wiebke","family":"Moskorz","sequence":"additional","affiliation":[{"name":"Institute of Virology, University Hospital D\u00fcsseldorf, Heinrich Heine University, Medical Faculty , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1578-8163","authenticated-orcid":false,"given":"Tina","family":"Senff","sequence":"additional","affiliation":[{"name":"Institute of Virology, University Hospital D\u00fcsseldorf, Heinrich Heine University, Medical Faculty , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eugen","family":"B\u00e4cker","sequence":"additional","affiliation":[{"name":"Institute of Virology, University Hospital D\u00fcsseldorf, Heinrich Heine University, Medical Faculty , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6708-4390","authenticated-orcid":false,"given":"Monika","family":"Lindemann","sequence":"additional","affiliation":[{"name":"Institute for Transfusion Medicine, University Hospital Essen, University of Duisburg-Essen , 45147 Essen ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert","family":"Zimmermann","sequence":"additional","affiliation":[{"name":"Institute of Virology, University Hospital D\u00fcsseldorf, Heinrich Heine University, Medical Faculty , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9553-1987","authenticated-orcid":false,"given":"Markus","family":"Uhrberg","sequence":"additional","affiliation":[{"name":"Institute for Transplantation Diagnostics and Cell Therapeutics, University Hospital D\u00fcsseldorf, Heinrich Heine University , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philipp A","family":"Lang","sequence":"additional","affiliation":[{"name":"Department of Molecular Medicine II, Medical Faculty, University Hospital D\u00fcsseldorf, Heinrich Heine University , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7799-3045","authenticated-orcid":false,"given":"J\u00f6rg","family":"Timm","sequence":"additional","affiliation":[{"name":"Institute of Virology, University Hospital D\u00fcsseldorf, Heinrich Heine University, Medical Faculty , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7788-4604","authenticated-orcid":false,"given":"Christine","family":"Cosmovici","sequence":"additional","affiliation":[{"name":"Institute of Virology, University Hospital D\u00fcsseldorf, Heinrich Heine University, Medical Faculty , 40225 D\u00fcsseldorf ,","place":["Germany"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2020,6,1]]},"reference":[{"key":"2025030417511970500_r1","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.cell.2014.12.020","article-title":"Variation in the human immune system is largely driven by non-heritable influences","volume":"160","author":"Brodin","year":"2015","journal-title":"Cell"},{"key":"2025030417511970500_r2","doi-asserted-by":"crossref","first-page":"3881","DOI":"10.4049\/jimmunol.1800171","article-title":"Cytomegalovirus: shape-shifting the immune system","volume":"200","author":"Picarda","year":"2018","journal-title":"J. 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