{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T08:59:05Z","timestamp":1790499545419,"version":"4.1.0"},"reference-count":23,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HA1284\/7-2"],"award-info":[{"award-number":["HA1284\/7-2"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["HA6145\/2-1"],"award-info":[{"award-number":["HA6145\/2-1"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>T follicular helper (Tfh) cells play a very important role in mounting a humoral response. Studies conducted in mouse models have revealed with good kinetic and spatial resolution the dynamics of these cells in germinal centers (GC) and their cross-talk with B cells upon an immune response. However, whether a similar migratory behavior is performed by human Tfh cells is unclear, as technology to track them in situ has been lacking. In this study, we combined traditional immunohistochemistry and real-time fluorescent imaging approaches on fresh human adenoid slices to provide static and dynamic information on Tfh cells. Our data indicate that GC light zones are composed of two distinct areas in terms of Tfh cell distribution and migration. In the outer GC light zones, Tfh cells migrate actively and with a high ability to form dynamic clusters showing intense and rapid reorganization. In these outer regions, Tfh cells demonstrate multiple interactions between each other. Conversely, in central regions of GC light zones, Tfh cells are much more static, forming long-lasting conjugates. These findings reveal for the first time, to our knowledge, the dynamic behavior whereby Tfh cells migrate in human GC and highlight the heterogeneity of GC for Tfh cell motility.<\/jats:p>","DOI":"10.4049\/jimmunol.1901475","type":"journal-article","created":{"date-parts":[[2020,7,22]],"date-time":"2020-07-22T14:57:45Z","timestamp":1595429865000},"page":"1248-1255","source":"Crossref","is-referenced-by-count":15,"title":["Landscape of T Follicular Helper Cell Dynamics in Human Germinal Centers"],"prefix":"10.1093","volume":"205","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4985-7254","authenticated-orcid":false,"given":"Emmanuel","family":"Donnadieu","sequence":"first","affiliation":[{"name":"D\u00e9partement Immunologie, Inflammation, et Infection, Institut Cochin, INSERM, U1016, CNRS, UMR8104, Universit\u00e9 de Paris , F-75014 Paris ,","place":["France"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5439-3909","authenticated-orcid":false,"given":"Kerstin Bianca","family":"Reisinger","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, Goethe University , 60590 Frankfurt am Main ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0300-7027","authenticated-orcid":false,"given":"Sonja","family":"Scharf","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, Goethe University , 60590 Frankfurt am Main ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Michel","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, Goethe University , 60590 Frankfurt am Main ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Bein","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, Goethe University , 60590 Frankfurt am Main ,","place":["Germany"]},{"name":"Reference and Consultant Center for Lymph Node and Lymphoma Diagnostics , 60590 Frankfurt ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Hansen","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, Goethe University , 60590 Frankfurt am Main ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas G","family":"Loth","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology, Head and Neck Surgery, University Hospital , 60590 Frankfurt am Main ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Flinner","sequence":"additional","affiliation":[{"name":"Frankfurt Institute for Advanced Studies , 60438 Frankfurt ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3424-1091","authenticated-orcid":false,"given":"Sylvia","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, Goethe University , 60590 Frankfurt am Main ,","place":["Germany"]},{"name":"Reference and Consultant Center for Lymph Node and Lymphoma Diagnostics , 60590 Frankfurt ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin-Leo","family":"Hansmann","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, Goethe University , 60590 Frankfurt am Main ,","place":["Germany"]},{"name":"Reference and Consultant Center for Lymph Node and Lymphoma Diagnostics , 60590 Frankfurt ,","place":["Germany"]},{"name":"Frankfurt Institute for Advanced Studies , 60438 Frankfurt ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2025030417504845900_r1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1146\/annurev-immunol-041015-055605","article-title":"Follicular helper T cells","volume":"34","author":"Vinuesa","year":"2016","journal-title":"Annu. 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