{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T03:58:47Z","timestamp":1782964727234,"version":"3.54.5"},"reference-count":33,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,9,1]],"date-time":"2020-09-01T00:00:00Z","timestamp":1598918400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes for Health Research","doi-asserted-by":"publisher","award":["MOP 114923"],"award-info":[{"award-number":["MOP 114923"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>There is currently no clinically effective vaccine against cutaneous leishmaniasis because of poor understanding of the Ags that elicit protective CD4+ T cell immunity. In this study, we identified a naturally processed peptide (DLD63\u201379) that is derived from Leishmania dihydrolipoyl dehydrogenase (DLD) protein. DLD is conserved in all pathogenic Leishmania species, is expressed by both the promastigote and amastigote stages of the parasite, and elicits strong CD4+ T cell responses in mice infected with L. major. We generated I-Ab-DLD63\u201379 tetramer and identified DLD-specific CD4+ T cells at clonal level. Following L. major infection, DLD63\u201379\u2013specific CD4+ T cells massively expanded and produced effector cytokines (IFN-\u03b3 and TNF). This was followed by a gradual contraction, stable maintenance following lesion resolution, and display of memory (recall) response following secondary challenge. Vaccination with rDLD protein induced strong protection in mice against virulent L. major challenge. Identification of Ags that elicit protective immunity and their responding Ag-specific T cells are critical steps necessary for developing effective vaccines and vaccination strategies against infectious agents, including protozoan parasites.<\/jats:p>","DOI":"10.4049\/jimmunol.2000338","type":"journal-article","created":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T20:34:40Z","timestamp":1596054880000},"page":"1355-1364","source":"Crossref","is-referenced-by-count":8,"title":["Identification of a Protective <i>Leishmania<\/i> Antigen Dihydrolipoyl Dehydrogenase and Its Responding CD4+ T Cells at Clonal Level"],"prefix":"10.1093","volume":"205","author":[{"given":"Zhirong","family":"Mou","sequence":"first","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aida F","family":"Barazandeh","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6964-1020","authenticated-orcid":false,"given":"Hiroshi","family":"Hamana","sequence":"additional","affiliation":[{"name":"Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama , Toyama 930-0194 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Kishi","sequence":"additional","affiliation":[{"name":"Department of Immunology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama , Toyama 930-0194 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoping","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ping","family":"Jia","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nnamdi","family":"Ikeogu","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chukwunonso","family":"Onyilagha","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4533-6272","authenticated-orcid":false,"given":"Gaurav","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jude E","family":"Uzonna","sequence":"additional","affiliation":[{"name":"Department of Immunology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]},{"name":"Department of Medical Microbiology, Max Rady College of Medicine, University of Manitoba , Winnipeg, Manitoba R3E 0T5 ,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,9,1]]},"reference":[{"key":"2025030417504852900_r1","doi-asserted-by":"crossref","first-page":"951","DOI":"10.1016\/S0140-6736(18)31204-2","article-title":"Leishmaniasis","volume":"392","author":"Burza","year":"2018","journal-title":"Lancet"},{"key":"2025030417504852900_r2","doi-asserted-by":"crossref","first-page":"604","DOI":"10.1038\/nrmicro2608","article-title":"Leishmaniasis: complexity at the host-pathogen interface","volume":"9","author":"Kaye","year":"2011","journal-title":"Nat. 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