{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,5]],"date-time":"2026-11-05T21:10:54Z","timestamp":1793913054670,"version":"4.8.0"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2021,10,1]],"date-time":"2021-10-01T00:00:00Z","timestamp":1633046400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81871538"],"award-info":[{"award-number":["No. 81871538"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No. 81670556"],"award-info":[{"award-number":["No. 81670556"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Youth Fund of National Natural Science Foundation of China","award":["No. 81900581"],"award-info":[{"award-number":["No. 81900581"]}]},{"name":"China postdoctoral Science Foundation on the 66th grant program","award":["No. F119NF0124"],"award-info":[{"award-number":["No. F119NF0124"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Sepsis is a life-threatening organ dysfunction caused by a dysfunctional host response to infection. Neutrophils play a protective role by releasing antibacterial proteins or by phagocytizing bacteria. However, excess neutrophils can induce tissue damage. Recently, a novel intercellular communication pathway involving extracellular vesicles (EVs) has garnered considerable attention. However, whether EVs secreted by macrophages mediate neutrophil recruitment to infected sites has yet to be studied. In this study, we assessed the chemotactic effect of EVs isolated from mouse Raw264.7 macrophages on mouse neutrophils and found that CXCL2 was highly expressed in these EVs. By regulating CXCL2 in Raw264.7 macrophages, we found that CXCL2 on macrophage EVs recruited neutrophils in vitro and in vivo. The CXCL2 EVs activated the CXCR2\/PKC\/NOX4 pathway and induced tissue damage. This study provides information regarding the mechanisms underlying neutrophil recruitment to tissues and proposes innovative strategies and targets for the treatment of sepsis.<\/jats:p>","DOI":"10.4049\/jimmunol.2100229","type":"journal-article","created":{"date-parts":[[2021,9,10]],"date-time":"2021-09-10T14:15:12Z","timestamp":1631283312000},"page":"2118-2128","source":"Crossref","is-referenced-by-count":55,"title":["Macrophagic Extracellular Vesicle CXCL2 Recruits and Activates the Neutrophil CXCR2\/PKC\/NOX4 Axis in Sepsis"],"prefix":"10.1093","volume":"207","author":[{"given":"Guozhen","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Emergency Medicine, Nanfang Hospital, Southern Medical University , Guangzhou ,","place":["China"]},{"name":"State Key Laboratory of Organ Failure Research, Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Nanfang Hospital, Southern Medical University , Guangzhou, Guangdong 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Guangdong Provincial Key Laboratory of Viral Hepatitis Research, Nanfang Hospital, Southern Medical University , Guangzhou, Guangdong ,","place":["China"]},{"name":"Guangdong Provincial Key Laboratory of Gastroenterology, Department of Gastroenterology, Nanfang Hospital, Southern Medical University , Guangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wanfu","family":"Cui","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Nanfang Hospital, Southern Medical University , Guangzhou ,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Nanfang Hospital, Southern Medical University , Guangzhou 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