{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T12:02:35Z","timestamp":1783512155940,"version":"3.55.0"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2022,5,1]],"date-time":"2022-05-01T00:00:00Z","timestamp":1651363200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["PS 156104"],"award-info":[{"award-number":["PS 156104"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000038","name":"Gouvernement du Canada | Natural Sciences and Engineering Research Council of Canada","doi-asserted-by":"publisher","award":["RGPIN-2017-05766"],"award-info":[{"award-number":["RGPIN-2017-05766"]}],"id":[{"id":"10.13039\/501100000038","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Li Ka Shing Institute of Virology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Several unique waves of \u03b3\u03b4 T cells are generated solely in the fetal\/neonatal thymus, whereas additional \u03b3\u03b4 T cell subsets are generated in adults. One intriguing feature of \u03b3\u03b4 T cell development is the coordination of differentiation and acquisition of effector function within the fetal thymus; however, it is less clear whether this paradigm holds true in adult animals. In this study, we investigated the relationship between maturation and thymic export of adult-derived \u03b3\u03b4 thymocytes in mice. In the Rag2pGFP model, immature (CD24+) \u03b3\u03b4 thymocytes expressed high levels of GFP whereas only a minority of mature (CD24\u2212) \u03b3\u03b4 thymocytes were GFP+. Similarly, most peripheral GFP+ \u03b3\u03b4 T cells were immature. Analysis of \u03b3\u03b4 recent thymic emigrants (RTEs) indicated that most \u03b3\u03b4 T cell RTEs were CD24+ and GFP+, and adoptive transfer experiments demonstrated that immature \u03b3\u03b4 thymocytes can mature outside the thymus. Mature \u03b3\u03b4 T cells largely did not recirculate to the thymus from the periphery; rather, a population of mature \u03b3\u03b4 thymocytes that produced IFN-\u03b3 or IL-17 remained resident in the thymus for at least 60 d. These data support the existence of two populations of \u03b3\u03b4 T cell RTEs in adult mice: a majority subset that is immature and matures in the periphery after thymic emigration, and a minority subset that completes maturation within the thymus prior to emigration. Additionally, we identified a heterogeneous population of resident \u03b3\u03b4 thymocytes of unknown functional importance. Collectively, these data shed light on the generation of the \u03b3\u03b4 T cell compartment in adult mice.<\/jats:p>","DOI":"10.4049\/jimmunol.2100360","type":"journal-article","created":{"date-parts":[[2022,4,8]],"date-time":"2022-04-08T14:01:39Z","timestamp":1649426499000},"page":"2131-2140","source":"Crossref","is-referenced-by-count":7,"title":["\u03b3\u03b4 Thymocyte Maturation and Emigration in Adult Mice"],"prefix":"10.1093","volume":"208","author":[{"given":"Kevin","family":"Joannou","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta , Edmonton, Alberta,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic P","family":"Golec","sequence":"additional","affiliation":[{"name":"Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health , Bethesda, MD"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5403-9179","authenticated-orcid":false,"given":"Haiguang","family":"Wang","sequence":"additional","affiliation":[{"name":"Center for Immunology, University of Minnesota , Minneapolis, MN"},{"name":"Department of Laboratory Medicine and Pathology, University of Minnesota , Minneapolis, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura M","family":"Henao-Caviedes","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta , Edmonton, Alberta,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3948-3444","authenticated-orcid":false,"given":"Julia F","family":"May","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta , Edmonton, Alberta,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9429-9087","authenticated-orcid":false,"given":"Rees G","family":"Kelly","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta , Edmonton, Alberta,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1839-5544","authenticated-orcid":false,"given":"Rigel","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta , Edmonton, Alberta,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9137-1146","authenticated-orcid":false,"given":"Stephen C","family":"Jameson","sequence":"additional","affiliation":[{"name":"Center for Immunology, University of Minnesota , Minneapolis, MN"},{"name":"Department of Laboratory Medicine and Pathology, University of Minnesota , Minneapolis, MN"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0122-3746","authenticated-orcid":false,"given":"Troy A","family":"Baldwin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Alberta , Edmonton, Alberta,","place":["Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,5,1]]},"reference":[{"key":"2025021009042528000_r1","doi-asserted-by":"crossref","first-page":"837","DOI":"10.1016\/0092-8674(88)90139-0","article-title":"Limited diversity of \u03b3\u03b4 antigen receptor genes of thy-l+ dendritic epidermal cells","volume":"55","author":"Asarnow","year":"1988","journal-title":"Cell"},{"key":"2025021009042528000_r2","doi-asserted-by":"crossref","first-page":"7935","DOI":"10.1073\/pnas.87.20.7935","article-title":"Selection of gamma delta T cells with canonical T-cell antigen receptors in fetal thymus","volume":"87","author":"Itohara","year":"1990","journal-title":"Proc. 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