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The C-terminal domain mediates phagocytosis of bacteria bound to the NTD. These two domains are linked by a surface-exposed loop at amino acids 231\u2013249 for human BPI, known as the \u201chinge region.\u201d Autoantibodies to human BPI are prevalent in many chronic lung diseases; their presence is strongly correlated with Pseudomonas aeruginosa and with worse lung function in patients with cystic fibrosis and bronchiectasis. Although prior literature has reported BPI neutralization effect with autoantibodies targeting either NTD or C-terminal domain, the functionality of BPI Ab to the hinge region has never been investigated. Here, we report that Ab responses to the BPI hinge region mediate a remarkably selective potentiation of BPI-dependent phagocytosis of P. aeruginosa with both human and murine neutrophils in vitro and in vivo. These findings indicate that autoantibodies to the BPI hinge region might enhance bacterial clearance.<\/jats:p>","DOI":"10.4049\/jimmunol.2100378","type":"journal-article","created":{"date-parts":[[2021,7,16]],"date-time":"2021-07-16T17:05:21Z","timestamp":1626455121000},"page":"777-783","source":"Crossref","is-referenced-by-count":4,"title":["Differential Enhancement of Neutrophil Phagocytosis by Anti\u2013Bactericidal\/Permeability-Increasing Protein Antibodies"],"prefix":"10.1093","volume":"207","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2139-9079","authenticated-orcid":false,"given":"Jomkuan","family":"Theprungsirikul","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth , Lebanon, NH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sladjana","family":"Skopelja-Gardner","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, Geisel School of Medicine at Dartmouth , Lebanon, NH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel M","family":"Wierzbicki","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth , Lebanon, NH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katherine J","family":"Sessions","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Department of Medicine, Geisel School of Medicine at Dartmouth , Lebanon, NH"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1382-7299","authenticated-orcid":false,"given":"William F C","family":"Rigby","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Geisel School of Medicine at Dartmouth , Lebanon, NH"},{"name":"Division of Rheumatology, Department of Medicine, Geisel School of Medicine at Dartmouth , Lebanon, NH"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,8,1]]},"reference":[{"key":"2025030501503372200_r1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1111\/j.1365-2249.2005.02834.x","article-title":"Clinical uses of intravenous immunoglobulin","volume":"142","author":"Jolles","year":"2005","journal-title":"Clin. 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