{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T05:06:18Z","timestamp":1739250378697,"version":"3.37.0"},"reference-count":56,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Toxoplasmosis, caused by Toxoplasma gondii, an apicomplexan parasite, infects all warm-blooded animals, including a third of the human population. Laboratory diagnosis of acute toxoplasmosis is based on the detection of anti\u2013T. gondii IgM and IgG and T. gondii nucleic acid; however, these assays have certain limitations. Circulating Ags (CAgs) are reliable diagnostic indicators of acute infection. In this study, we established a model of acute T. gondii infection in Large White pigs. CAg levels peaked between 3 and 5 d after inoculation, and 28 CAgs were identified using an immunoprecipitation-shotgun approach, among which dolichol-phosphate-mannose synthase family protein (TgDPM), C3HC zinc finger-like protein (TgZFLP3), and ribosomal protein RPL7 (TgRPL7) were selected to further investigate their value in the diagnosis of acute toxoplasmosis. Immunofluorescence assays revealed that TgDPM and TgRPL7 were localized in the membrane surface, while TgZFLP3 was localized in the apical end. Western blotting revealed the presence of the three proteins in the serum during acute infection. Indirect ELISA results indicate that TgZFLP3 is likely to be a novel candidate for the diagnosis of acute toxoplasmosis. However, these three proteins may not be useful as candidate vaccines against toxoplasmosis owing to their low protective ability. In addition, deletion of the zflp3 gene partially attenuated virulence in Kunming mice. Collectively, we identified 28 CAgs in the serum of piglets with experimental acute toxoplasmosis and confirmed that TgZFLP3 is a potential biomarker for acute T. gondii infection. The results of this study provide data to improve the detection efficiency of acute toxoplasmosis.<\/jats:p>","DOI":"10.4049\/jimmunol.2100640","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T14:20:26Z","timestamp":1641997226000},"page":"697-706","source":"Crossref","is-referenced-by-count":1,"title":["Diagnostic Value of Circulating Antigens in the Serum of Piglets with Experimental Acute Toxoplasmosis"],"prefix":"10.1093","volume":"208","author":[{"given":"Qi","family":"Liu","sequence":"first","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science , Shanghai,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Man-yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science , Shanghai,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Zhao","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science , Shanghai,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science , Shanghai,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Jiang","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science , Shanghai,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-ling","family":"Geng","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science , Shanghai,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Quan","family":"Wang","sequence":"additional","affiliation":[{"name":"Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Science , Shanghai,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"key":"2025021008522054900_r1","doi-asserted-by":"crossref","first-page":"1217","DOI":"10.1016\/S0020-7519(00)00124-7","article-title":"Toxoplasma gondii: from animals to humans","volume":"30","author":"Tenter","year":"2000","journal-title":"Int. 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Microbiol."},{"key":"2025021008522054900_r5","doi-asserted-by":"crossref","first-page":"2108","DOI":"10.3389\/fmicb.2018.02108","article-title":"Prevalence, risk factors, and genotypes of Toxoplasma gondii in food animals and humans (2000\u20132017) from China","volume":"9","author":"Dong","year":"2018","journal-title":"Front. Microbiol."},{"key":"2025021008522054900_r6","doi-asserted-by":"crossref","first-page":"1877","DOI":"10.1017\/S0031182009990941","article-title":"Evidence for high levels of vertical transmission in Toxoplasma gondii","volume":"136","author":"Hide","year":"2009","journal-title":"Parasitology"},{"key":"2025021008522054900_r7","doi-asserted-by":"crossref","first-page":"766","DOI":"10.1038\/nrmicro2858","article-title":"Modulation of innate immunity by Toxoplasma gondii virulence effectors","volume":"10","author":"Hunter","year":"2012","journal-title":"Nat. Rev. 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Immunol."},{"key":"2025021008522054900_r14","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.parint.2010.11.002","article-title":"Development of an immunochromatographic strip for the rapid detection of Toxoplasma gondii circulating antigens","volume":"60","author":"Wang","year":"2011","journal-title":"Parasitol. Int."},{"key":"2025021008522054900_r15","doi-asserted-by":"crossref","first-page":"336","DOI":"10.4269\/ajtmh.1995.52.336","article-title":"Detection of circulating antigens of Toxoplasma gondii in human infection","volume":"52","author":"Hafid","year":"1995","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"2025021008522054900_r16","doi-asserted-by":"crossref","first-page":"530","DOI":"10.3923\/pjbs.2009.530.533","article-title":"Evaluation of excreted\/secreted antigens derived from peritoneal of Toxoplasma infected small mice to detect IgG against Toxoplasma","volume":"12","author":"Abdollahi","year":"2009","journal-title":"Pak. J. Biol. 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Hyg."},{"key":"2025021008522054900_r30","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1016\/j.meegid.2018.04.033","article-title":"Bioinformatics analysis of ROP8 protein to improve vaccine design against Toxoplasma gondii","volume":"62","author":"Foroutan","year":"2018","journal-title":"Infect. Genet. Evol."},{"key":"2025021008522054900_r31","doi-asserted-by":"crossref","first-page":"933","DOI":"10.1016\/S0092-8674(00)81071-5","article-title":"Toxoplasma invasion of mammalian cells is powered by the actin cytoskeleton of the parasite","volume":"84","author":"Dobrowolski","year":"1996","journal-title":"Cell"},{"key":"2025021008522054900_r32","first-page":"168","article-title":"Toxoplasma gondii elongation factor 1-alpha (TgEF-1\u03b1) is a novel vaccine candidate antigen against toxoplasmosis","volume":"8","author":"Wang","year":"2017","journal-title":"Front. 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Parasitol."},{"key":"2025021008522054900_r35","doi-asserted-by":"crossref","first-page":"2149","DOI":"10.1093\/emboj\/21.9.2149","article-title":"Toxoplasma gondii myosin A and its light chain: a fast, single-headed, plus-end-directed motor","volume":"21","author":"Herm-G\u00f6tz","year":"2002","journal-title":"EMBO J."},{"key":"2025021008522054900_r36","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1016\/j.ijpara.2018.01.009","article-title":"Phosphoinositides and their functions in apicomplexan parasites","volume":"48","author":"Wengelnik","year":"2018","journal-title":"Int. J. Parasitol."},{"key":"2025021008522054900_r37","doi-asserted-by":"crossref","first-page":"301","DOI":"10.1128\/mBio.00301-18","article-title":"TgTKL1 is a unique plant-like nuclear kinase that plays an essential role in acute toxoplasmosis","volume":"9","author":"Varberg","year":"2018","journal-title":"MBio"},{"key":"2025021008522054900_r38","doi-asserted-by":"crossref","first-page":"1562","DOI":"10.4049\/jimmunol.1900410","article-title":"Toxoplasma gondii tkl1 deletion mutant is a promising vaccine against acute, chronic, and congenital toxoplasmosis in mice","volume":"204","author":"Wang","year":"2020","journal-title":"J. Immunol."},{"key":"2025021008522054900_r39","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1016\/j.bios.2015.04.035","article-title":"A novel dynamic flow immunochromatographic test (DFICT) using gold nanoparticles for the serological detection of Toxoplasma gondii infection in dogs and cats","volume":"72","author":"Jiang","year":"2015","journal-title":"Biosens. Bioelectron."},{"key":"2025021008522054900_r40","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1007\/BF01691497","article-title":"Toxoplasma gondii surface antigen-1 in sera of HIV-infected patients as an indicator of reactivated toxoplasmosis","volume":"14","author":"Letillois","year":"1995","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"2025021008522054900_r41","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1017\/S0031182014001978","article-title":"Identification of Toxoplasma gondii antigens associated with different types of infection by serum antibody profiling","volume":"142","author":"Felgner","year":"2015","journal-title":"Parasitology"},{"key":"2025021008522054900_r42","doi-asserted-by":"crossref","first-page":"2265","DOI":"10.1016\/j.bbagen.2015.08.012","article-title":"Dolichol phosphate mannose synthase from the pathogenic yeast Candida albicans is a multimeric enzyme","volume":"1850","author":"Juchimiuk","year":"2015","journal-title":"Biochim. Biophys. Acta"},{"key":"2025021008522054900_r43","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1146\/annurev-biochem-060614-033917","article-title":"Functions of ribosomal proteins in assembly of eukaryotic ribosomes in vivo","volume":"84","author":"de la Cruz","year":"2015","journal-title":"Annu. Rev. Biochem."},{"key":"2025021008522054900_r44","doi-asserted-by":"crossref","first-page":"861","DOI":"10.1016\/j.bbagen.2008.03.005","article-title":"Dolichol-phosphate mannose synthase: structure, function and regulation","volume":"1780","author":"Maeda","year":"2008","journal-title":"Biochim. Biophys. Acta"},{"key":"2025021008522054900_r45","doi-asserted-by":"crossref","first-page":"23","DOI":"10.1016\/j.vetpar.2004.11.002","article-title":"Production of antibodies in murine mucosal immunization with Toxoplasma gondii excreted\/secreted antigens","volume":"128","author":"Hafid","year":"2005","journal-title":"Vet. Parasitol."},{"key":"2025021008522054900_r46","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1590\/S0074-02762009000200019","article-title":"Vaccines against Toxoplasma gondii: challenges and opportunities","volume":"104","author":"Jongert","year":"2009","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"2025021008522054900_r47","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1016\/j.actatropica.2012.08.013","article-title":"Immunization with excreted\/secreted proteins in AS\/n mice activating cellular and humoral response against Toxoplasma gondii infection","volume":"124","author":"Costa-Silva","year":"2012","journal-title":"Acta Trop."},{"key":"2025021008522054900_r48","doi-asserted-by":"crossref","first-page":"1463","DOI":"10.3923\/javaa.2010.1463.1468","article-title":"Sequence variation in Toxoplasma gondii MIC4 gene and protective effect of an MIC4 DNA vaccine in a murine model against toxoplasmosis","volume":"9","author":"Peng","year":"2010","journal-title":"J. Anim. Vet. 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