{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T10:47:53Z","timestamp":1788346073540,"version":"build-2803163510"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000050","name":"HHS | NIH | National Heart, Lung, and Blood Institute","doi-asserted-by":"publisher","award":["P01 HL132819"],"award-info":[{"award-number":["P01 HL132819"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>RBC transfusion therapy is essential for the treatment of anemia. A serious complication of transfusion is the development of non-ABO alloantibodies to polymorphic RBC Ags; yet, mechanisms of alloantibody formation remain unclear. Storage of mouse RBCs before transfusion increases RBC immunogenicity through an unknown mechanism. We previously reported that sterile, stored mouse RBCs activate splenic dendritic cells (DCs), which are required for alloimmunization. Here we transfused mice with allogeneic RBCs to test whether stored RBCs activate pattern recognition receptors (PRRs) on recipient DCs to induce adaptive immunity. TLRs are a class of PRRs that regulate DC activation, which signal through two adapter molecules: MyD88 and TRIF. We show that the inflammatory cytokine response, DC activation and migration, and the subsequent alloantibody response to transfused RBCs require MyD88 but not TRIF, suggesting that a restricted set of PRRs are responsible for sensing RBCs and triggering alloimmunization.<\/jats:p>","DOI":"10.4049\/jimmunol.2100784","type":"journal-article","created":{"date-parts":[[2022,1,17]],"date-time":"2022-01-17T19:25:14Z","timestamp":1642447514000},"page":"991-997","source":"Crossref","is-referenced-by-count":19,"title":["Innate and Adaptive Immunity to Transfused Allogeneic RBCs in Mice Requires MyD88"],"prefix":"10.1093","volume":"208","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5544-9639","authenticated-orcid":false,"given":"Arielle","family":"Soldatenko","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura R","family":"Hoyt","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7881-5160","authenticated-orcid":false,"given":"Samuele","family":"Calabro","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1979-0554","authenticated-orcid":false,"given":"Steven M","family":"Lewis","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9107-8281","authenticated-orcid":false,"given":"Antonia E","family":"Gallman","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5663-9290","authenticated-orcid":false,"given":"Krystalyn E","family":"Hudson","sequence":"additional","affiliation":[{"name":"Department of Pathology and Cell Biology, Columbia University Irving Medical Center , New York, NY"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean R","family":"Stowell","sequence":"additional","affiliation":[{"name":"Department of Pathology, Brigham and Women\u2019s Hospital , Boston, MA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chance J","family":"Luckey","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Virginia , Charlottesville, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James C","family":"Zimring","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of Virginia , Charlottesville, VA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3703-7635","authenticated-orcid":false,"given":"Manjula","family":"Santhanakrishnan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Pediatrics, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7928-3132","authenticated-orcid":false,"given":"Jeanne E","family":"Hendrickson","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Pediatrics, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1244-208X","authenticated-orcid":false,"given":"Stephanie C","family":"Eisenbarth","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yale University School of Medicine , New Haven, CT"},{"name":"Department of Immunobiology, Yale University School of Medicine , New Haven, CT"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,2,15]]},"reference":[{"key":"2025021008543052700_r1","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.tmrv.2014.04.008","article-title":"Red blood cell alloimmunization mitigation strategies","volume":"28","author":"Hendrickson","year":"2014","journal-title":"Transfus. 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Haematol."},{"key":"2025021008543052700_r4","doi-asserted-by":"crossref","first-page":"1378","DOI":"10.1111\/trf.12940","article-title":"Alloimmunization in sickle cell disease: changing antibody specificities and association with chronic pain and decreased survival","volume":"55","author":"Telen","year":"2015","journal-title":"Transfusion"},{"key":"2025021008543052700_r5","doi-asserted-by":"crossref","first-page":"1374","DOI":"10.1111\/trf.13065","article-title":"Routine non-ABO blood group antigen genotyping in sickle cell disease: the new frontier in pretransfusion testing?","volume":"55","author":"Tormey","year":"2015","journal-title":"Transfusion"},{"key":"2025021008543052700_r6","doi-asserted-by":"crossref","first-page":"1340","DOI":"10.1002\/ajh.24908","article-title":"Incidence and predictive score for delayed hemolytic transfusion reaction in adult patients with sickle cell disease","volume":"92","author":"Narbey","year":"2017","journal-title":"Am. J. 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