{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:36:20Z","timestamp":1790840180678,"version":"4.1.0"},"reference-count":60,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,2,1]],"date-time":"2022-02-01T00:00:00Z","timestamp":1643673600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["AI057753"],"award-info":[{"award-number":["AI057753"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007172","name":"Cincinnati Children\u2019s Hospital Medical Center","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007172","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The precursors of TCR\u03b1\u03b2+CD8\u03b1\u03b1+ intraepithelial lymphocytes (IEL) arise in the thymus through a complex process of agonist selection. We and others have shown that the proapoptotic protein, Bim, is critical to limit the number of thymic IEL precursors (IELp), as loss of Bim at the CD4+CD8+ double-positive stage of development drastically increases IELp. The factors determining this cell death versus survival decision remain largely unknown. In this study, we used CD4CreBcl2f\/f mice to define the role of the antiapoptotic protein Bcl-2 and CD4CreBcl2f\/fBimf\/f mice to determine the role of Bcl-2 in opposing Bim to promote survival of IELp. First, in wild-type mice, we defined distinct subpopulations within PD-1+CD122+ IELp, based on their expression of Runx3 and \u03b14\u03b27. Coexpression of \u03b14\u03b27 and Runx3 marked IELp that were most dependent upon Bcl-2 for survival. Importantly, the additional loss of Bim restored Runx3+\u03b14\u03b27+ IELp, showing that Bcl-2 antagonizes Bim to enable IELp survival. Further, the loss of thymic IELp in CD4CreBcl2f\/f mice also led to a dramatic loss of IEL in the gut, and the additional loss of Bim restored gut IEL. The loss of gut IEL was due to both reduced seeding by IELp from the thymus as well as a requirement for Bcl-2 for peripheral IEL survival. Together, these findings highlight subset-specific and temporal roles for Bcl-2 in driving the survival of TCR\u03b1\u03b2+CD8\u03b1\u03b1+ IEL and thymic IELp.<\/jats:p>","DOI":"10.4049\/jimmunol.2100975","type":"journal-article","created":{"date-parts":[[2022,1,7]],"date-time":"2022-01-07T12:05:24Z","timestamp":1641557124000},"page":"651-659","source":"Crossref","is-referenced-by-count":10,"title":["Bcl-2 Is Necessary to Counteract Bim and Promote Survival of TCR\u03b1\u03b2+CD8\u03b1\u03b1+ Intraepithelial Lymphocyte Precursors in the Thymus"],"prefix":"10.1093","volume":"208","author":[{"given":"Sharmila","family":"Shanmuganad","sequence":"first","affiliation":[{"name":"Immunology Graduate Program, Cincinnati Children\u2019s Hospital Medical Center and the University of Cincinnati College of Medicine , Cincinnati, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4446-6198","authenticated-orcid":false,"given":"Sarah A","family":"Hummel","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Cincinnati Children\u2019s Hospital Medical Center and the University of Cincinnati College of Medicine , Cincinnati, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian","family":"Varghese","sequence":"additional","affiliation":[{"name":"Division of Immunobiology, Cincinnati Children\u2019s Hospital Medical Center and the University of Cincinnati College of Medicine , Cincinnati, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0421-8483","authenticated-orcid":false,"given":"David A","family":"Hildeman","sequence":"additional","affiliation":[{"name":"Immunology Graduate Program, Cincinnati Children\u2019s Hospital Medical Center and the University of Cincinnati College of Medicine , Cincinnati, OH"},{"name":"Division of Immunobiology, Cincinnati Children\u2019s Hospital Medical Center and the University of Cincinnati College of Medicine , Cincinnati, OH"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,2,1]]},"reference":[{"key":"2025021008522976000_r1","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1038\/nri3007","article-title":"The light and dark sides of intestinal intraepithelial lymphocytes","volume":"11","author":"Cheroutre","year":"2011","journal-title":"Nat. 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