{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:43:46Z","timestamp":1791337426179,"version":"4.3.1"},"reference-count":53,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T00:00:00Z","timestamp":1648771200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100000925","name":"Department of Health | National Health and Medical Research Council","doi-asserted-by":"publisher","award":["GNT1104036"],"award-info":[{"award-number":["GNT1104036"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008322","name":"CSL Behring","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In stroke patients, infection is a significant contributor to morbidity and mortality. Moreover, older stroke patients show an increased risk of developing stroke-associated infection, although the mechanisms underlying this increased susceptibility to infection are unknown. In this study, using an experimental mouse model of ischemic stroke, we showed that older (12\u201315 mo of age) mice had elevated lung bacterial infection and inflammatory damage after stroke when compared with young (8\u201310 wk of age) counterparts, despite undergoing the same degree of brain injury. Intravital microscopy of the lung microvasculature revealed that in younger mice, stroke promoted neutrophil arrest in pulmonary microvessels, but this response was not seen in older poststroke mice. In addition, bacterial phagocytosis by neutrophils in the lung microvasculature was reduced by both aging and stroke, such that neutrophils in aged poststroke mice showed the greatest impairment in this function. Analysis of neutrophil migration in vitro and in the cremaster muscle demonstrated that stroke alone did not negatively impact neutrophil migration, but that the combination of increased age and stroke led to reduced effectiveness of neutrophil chemotaxis. Transcriptomic analysis of pulmonary neutrophils using RNA sequencing identified 79 genes that were selectively altered in the context of combined aging and stroke, and they were associated with pathways that control neutrophil chemotaxis. Taken together, the findings of this study show that stroke in older animals results in worsening of neutrophil antibacterial responses and changes in neutrophil gene expression that have the potential to underpin elevated risk of stroke-associated infection in the context of increased age.<\/jats:p>","DOI":"10.4049\/jimmunol.2100997","type":"journal-article","created":{"date-parts":[[2022,4,1]],"date-time":"2022-04-01T10:55:41Z","timestamp":1648810541000},"page":"2019-2028","source":"Crossref","is-referenced-by-count":12,"title":["Lung Imaging Reveals Stroke-Induced Impairment in Pulmonary Intravascular Neutrophil Function, a Response Exacerbated with Aging"],"prefix":"10.1093","volume":"208","author":[{"given":"Shu Wen","family":"Wen","sequence":"first","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University , Clayton, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond","family":"Shim","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University , Clayton, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pam","family":"Hall","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University , Clayton, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5704-0212","authenticated-orcid":false,"given":"Justin","family":"Bedo","sequence":"additional","affiliation":[{"name":"Bioinformatics Division, Walter and Eliza Hall Institute of Medical Research , Parkville, Victoria,","place":["Australia"]},{"name":"School of Computing and Information Systems, The University of Melbourne , Parkville, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny L","family":"Wilson","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University , Clayton, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7953-6427","authenticated-orcid":false,"given":"Alyce J","family":"Nicholls","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University , Clayton, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2354-357X","authenticated-orcid":false,"given":"Michael J","family":"Hickey","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University , Clayton, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Connie H Y","family":"Wong","sequence":"additional","affiliation":[{"name":"Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences, Monash University , Clayton, Victoria,","place":["Australia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,4,15]]},"reference":[{"key":"2025021009033151100_r1","doi-asserted-by":"crossref","first-page":"1097","DOI":"10.1161\/01.STR.0000258346.68966.9d","article-title":"Infection after acute ischemic stroke: a manifestation of brain-induced immunodepression","volume":"38","author":"Chamorro","year":"2007","journal-title":"Stroke"},{"key":"2025021009033151100_r2","doi-asserted-by":"crossref","first-page":"1338","DOI":"10.1212\/WNL.0b013e31823152b1","article-title":"Risk factors, inpatient care, and outcomes of pneumonia after ischemic stroke","volume":"77","author":"Finlayson","year":"2011","journal-title":"Neurology"},{"key":"2025021009033151100_r3","doi-asserted-by":"crossref","first-page":"110","DOI":"10.1186\/1471-2377-11-110","article-title":"Post-stroke infection: a systematic review and meta-analysis","volume":"11","author":"Westendorp","year":"2011","journal-title":"BMC Neurol."},{"key":"2025021009033151100_r4","doi-asserted-by":"crossref","first-page":"2756","DOI":"10.1161\/01.STR.0000190056.76543.eb","article-title":"Dysphagia after stroke: incidence, diagnosis, and pulmonary complications","volume":"36","author":"Martino","year":"2005","journal-title":"Stroke"},{"key":"2025021009033151100_r5","doi-asserted-by":"crossref","first-page":"1323","DOI":"10.1007\/s00415-007-0520-0","article-title":"Predictors of pneumonia in acute stroke patients admitted to a neurological intensive care unit","volume":"254","author":"Walter","year":"2007","journal-title":"J. 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