{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,26]],"date-time":"2025-10-26T22:55:51Z","timestamp":1761519351625,"version":"3.37.3"},"reference-count":36,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2022,9,1]],"date-time":"2022-09-01T00:00:00Z","timestamp":1661990400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Melanoma-associated Ag (MAGE)-C2, an immunogenic cancer germline (testis) Ag, is highly expressed by various tumor cells, thymic medullary epithelial cells, and germ cells. In this study, we aimed to explore the immunologic properties of MAGE-C2\u2013specific CD8+ T cells and the relationship of its TCR \u03b2-chain V region (TCR v\u03b2) subfamily distribution to prognosis of patients with esophageal cancer. PBMCs and tumor-infiltrating lymphocytes expanded by CD3\/CD28 Dynabeads and MAGE-C2 peptides in vitro resulted in the induction of lysosome-associated membrane protein-1 (LAMP-1 or CD107a) on the cell surface and the production of IFN-\u03b3 by MAGE-C2\u2013specific CD8+ T cells. We found differential TCR v\u03b2 subfamily distribution among flow-sorted CD107a+IFN-\u03b3+ and CD107a\u2212IFN-\u03b3\u2212 CD8+ T cells. The proportion of CD107a+ and\/or IFN-\u03b3+ tetramer+ CD8+ T cells was lower in patients with lymph node metastasis, late tumor stage, and poorly differentiated state (p &amp;lt; 0.05). T-box transcription factor was positively correlated with CD107a and IFN-\u03b3. Kaplan\u2013Meier analysis showed that patients whose MAGE-C2\u2013specific CD8+ T cells expressed high CD107a and\/or IFN-\u03b3 had a longer survival time when compared with patients whose MAGE-C2\u2013specific CD8+ T cells expressed low levels of CD107a and\/or IFN-\u03b3. Moreover, analysis of TCR v\u03b2 subfamily distribution revealed that a higher frequency of TCR v\u03b216 in MAGE-C2\u2013specific CD8+ T cells was positively correlated with a better prognosis. These results suggest that the presence of functional MAGE-C2\u2013specific CD8+ T cells had an independent prognostic impact on the survival of patients with esophageal cancer.<\/jats:p>","DOI":"10.4049\/jimmunol.2101182","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T04:15:48Z","timestamp":1672719348000},"page":"1039-1047","source":"Crossref","is-referenced-by-count":3,"title":["Clinical Correlation of Function and TCR v\u03b2 Diversity of MAGE-C2\u2013Specific CD8+ T Cell Response in Esophageal Cancer"],"prefix":"10.1093","volume":"209","author":[{"given":"Pupu","family":"Li","sequence":"first","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinfeng","family":"Chen","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu","family":"Ping","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guohui","family":"Qin","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Huang","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qitai","family":"Zhao","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanan","family":"Chen","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liping","family":"Wang","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengli","family":"Yang","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9861-4681","authenticated-orcid":false,"given":"Yi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Biotherapy Center and Cancer Center, The First Affiliated Hospital of Zhengzhou University , Zhengzhou, Henan,","place":["China"]},{"name":"School of Life Sciences, Zhengzhou University , Zhengzhou, Henan,","place":["China"]},{"name":"Henan Key Laboratory for Tumor Immunology and Biotherapy , Zhengzhou, Henan,","place":["China"]},{"name":"State Key Laboratory of Esophageal Cancer Prevention & Treatment, Zhengzhou University , Zhengzhou, Henan,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,9,15]]},"reference":[{"key":"2025021008303640200_r1","doi-asserted-by":"crossref","first-page":"454","DOI":"10.1016\/j.ccell.2020.07.013","article-title":"Identifying and targeting human tumor antigens for T cell-based immunotherapy of solid tumors","volume":"38","author":"Leko","year":"2020","journal-title":"Cancer Cell"},{"key":"2025021008303640200_r2","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1186\/s13045-021-01081-7","article-title":"HLA-A2.1-restricted ECM1-derived epitope LA through DC cross-activation priming CD8+ T and NK cells: a novel therapeutic tumour vaccine. 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