{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T05:27:48Z","timestamp":1787030868924,"version":"build-2736575974"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,8,1]],"date-time":"2022-08-01T00:00:00Z","timestamp":1659312000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01 EY14362"],"award-info":[{"award-number":["R01 EY14362"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["F31 EY032312-02"],"award-info":[{"award-number":["F31 EY032312-02"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Pseudomonas aeruginosa is an important cause of dermal, pulmonary, and ocular disease. Our studies have focused on P. aeruginosa infections of the cornea (keratitis) as a major cause of blinding microbial infections. The infection leads to an influx of innate immune cells, with neutrophils making up to 90% of recruited cells during early stages. We previously reported that the proinflammatory cytokines IL-1\u03b1 and IL-1\u03b2 were elevated during infection. Compared with wild-type (WT), infected Il1b\u2212\/\u2212 mice developed more severe corneal disease that is associated with impaired bacterial killing as a result of defective neutrophil recruitment. We also reported that neutrophils are an important source of IL-1\u03b1 and IL-1\u03b2, which peaked at 24 h postinfection. To examine the role of IL-1\u03b1 compared with IL-1\u03b2 in P. aeruginosa keratitis, we inoculated corneas of C57BL\/6 (WT), Il1a\u2212\/\u2212, Il1b\u2212\/\u2212, and Il1a\u2212\/\u2212Il1b\u2212\/\u2212 (double-knockout) mice with 5 \u00d7 104 ExoS-expressing P. aeruginosa. Il1b\u2212\/\u2212 and double-knockout mice have significantly higher bacterial burden that was consistent with delayed neutrophil and monocyte recruitment to the corneas. Surprisingly, Il1a\u2212\/\u2212 mice had the opposite phenotype with enhanced bacteria clearance compared with WT mice. Although there were no significant differences in neutrophil recruitment, Il1a\u2212\/\u2212 neutrophils displayed a more proinflammatory transcriptomic profile compared to WT with elevations in C1q expression that likely caused the phenotypic differences observed. To our knowledge, our findings identify a novel, non-redundant role for IL-1\u03b1 in impairing bacterial clearance.<\/jats:p>","DOI":"10.4049\/jimmunol.2200110","type":"journal-article","created":{"date-parts":[[2023,1,2]],"date-time":"2023-01-02T23:15:29Z","timestamp":1672701329000},"page":"548-558","source":"Crossref","is-referenced-by-count":17,"title":["Differential Roles for IL-1\u03b1 and IL-1\u03b2 in\n                    <i>Pseudomonas aeruginosa<\/i>\n                    Corneal Infection"],"prefix":"10.1093","volume":"209","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5568-0723","authenticated-orcid":false,"given":"Bridget","family":"Ratitong","sequence":"first","affiliation":[{"name":"Department of Physiology and Biophysics, University of California , Irvine, Irvine, CA"},{"name":"Institute for Immunology, University of California , Irvine, Irvine, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9381-780X","authenticated-orcid":false,"given":"Michaela E","family":"Marshall","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California , Irvine, Irvine, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6401-6816","authenticated-orcid":false,"given":"Morgan A","family":"Dragan","sequence":"additional","affiliation":[{"name":"Institute for Immunology, University of California , Irvine, Irvine, CA"},{"name":"Department of Biological Chemistry, University of California , Irvine, Irvine, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2839-9319","authenticated-orcid":false,"given":"Charissa M","family":"Anunciado","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California , Irvine, Irvine, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9960-4079","authenticated-orcid":false,"given":"Serena","family":"Abbondante","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University of California , Irvine, Irvine, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0137-7582","authenticated-orcid":false,"given":"Eric","family":"Pearlman","sequence":"additional","affiliation":[{"name":"Department of Physiology and Biophysics, University of California , Irvine, Irvine, CA"},{"name":"Institute for Immunology, University of California , Irvine, Irvine, CA"},{"name":"Department of Ophthalmology, University of California , Irvine, Irvine, CA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,8,1]]},"reference":[{"key":"2025021008230791000_r1","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/j.survophthal.2018.12.003","article-title":"The persistent dilemma of microbial keratitis: global burden, diagnosis, and antimicrobial resistance","volume":"64","author":"Ung","year":"2019","journal-title":"Surv. 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J."},{"key":"2025021008230791000_r4","doi-asserted-by":"crossref","first-page":"e64867","DOI":"10.1371\/journal.pone.0064867","article-title":"Host response and bacterial virulence factor expression in Pseudomonas aeruginosa and Streptococcus pneumoniae corneal ulcers","volume":"8","author":"Karthikeyan","year":"2013","journal-title":"PLoS One"},{"key":"2025021008230791000_r5","doi-asserted-by":"crossref","first-page":"4231","DOI":"10.4049\/jimmunol.1201447","article-title":"Cutting edge: IL-1\u03b2 processing during Pseudomonas aeruginosa infection is mediated by neutrophil serine proteases and is independent of NLRC4 and caspase-1","volume":"189","author":"Karmakar","year":"2012","journal-title":"J. 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