{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T22:22:52Z","timestamp":1769638972615,"version":"3.49.0"},"reference-count":64,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2022,11,1]],"date-time":"2022-11-01T00:00:00Z","timestamp":1667260800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001407","name":"Department of Biotechnology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001407","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004423","name":"World Health Organization","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004423","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Commercial dengue virus (DENV) nonstructural-1 (NS1) Ag detection immunoassays often perform poorly, particularly in secondary DENV infection. To develop a highly sensitive NS1 ELISA, we generated a large repertoire of anti-DENV NS1 mouse mAbs (n = 95) that falls into 36 mAb classes based on binding specificities. The identified mAb pair, capable of efficiently detecting NS1 from four DENV serotypes in an immunoassay, was selected based on multiparametric analysis. The selected mAbs have subnanomolar affinities for NS1 with recognition sites outside the immunodominant wing domain. The assay was converted to an ELISA kit, which showed higher analytical sensitivity (3-fold to 83-fold) for NS1 from four DENV serotypes than commercial Platelia NS1 ELISA (Bio-Rad Laboratories). Compared to RT-PCR, the developed NS1 ELISA showed 78.57% (66 of 84) sensitivity, whereas Platelia NS1 ELISA showed a sensitivity of 60.71% (51 of 84). In a subgroup of RT-PCR\u2013positive secondary dengue samples, our ELISA showed a sensitivity of 70.18% (40 of 57), whereas Platelia ELISA detected only 47.37% (27 of 57) samples. Furthermore, unlike Platelia ELISA, our test equally detected NS1 from four serotypes; Platelia ELISA performed poorly for the DENV-2 serotype, in which only 8 of 21 (38.10%) samples were detected compared with 17 of 21 (80.95%) in our ELISA. Moreover, our ELISA showed 100% specificity in 342 challenging dengue-negative samples. The large and diverse mAb repertoire generated against DENV NS1 and the appropriate selection of mAbs allowed us to establish an ELISA that can efficiently detect NS1 Ag even in secondary dengue and without serotype level bias.<\/jats:p>","DOI":"10.4049\/jimmunol.2200251","type":"journal-article","created":{"date-parts":[[2023,1,3]],"date-time":"2023-01-03T05:02:36Z","timestamp":1672722156000},"page":"2054-2067","source":"Crossref","is-referenced-by-count":4,"title":["Generation of a Large Repertoire of Monoclonal Antibodies against Dengue Virus NS1 Antigen and the Development of a Novel NS1 Detection Enzyme-Linked Immunosorbent Assay"],"prefix":"10.1093","volume":"209","author":[{"given":"Farha","family":"Mehdi","sequence":"first","affiliation":[{"name":"Translational Health Science and Technology Institute , Faridabad, Haryana,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shirlie Roy","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute , Faridabad, Haryana,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarla","family":"Yadav","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute , Faridabad, Haryana,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jitendra Singh","family":"Kansana","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute , Faridabad, Haryana,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sangita Kumari","family":"Sinha","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute , Faridabad, Haryana,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soon Jyoti","family":"Das","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute , Faridabad, Haryana,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rakesh","family":"Lodha","sequence":"additional","affiliation":[{"name":"All India Institute of Medical Sciences , New Delhi, Delhi,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4561-8456","authenticated-orcid":false,"given":"John Antony Jude","family":"Prakash","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Christian Medical College , Vellore, Tamil Nadu,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Urpo","family":"Lamminim\u00e4ki","sequence":"additional","affiliation":[{"name":"Department of Life Technologies\/Biotechnology, University of Turku , Turku,","place":["Finland"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1669-4310","authenticated-orcid":false,"given":"Gaurav","family":"Batra","sequence":"additional","affiliation":[{"name":"Translational Health Science and Technology Institute , Faridabad, Haryana,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"2025021008405821900_r1","doi-asserted-by":"crossref","first-page":"1423","DOI":"10.1056\/NEJMra1110265","article-title":"Dengue","volume":"366","author":"Simmons","year":"2012","journal-title":"N. 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Microbiol."},{"key":"2025021008405821900_r8","doi-asserted-by":"crossref","first-page":"395","DOI":"10.1146\/annurev-med-050715-105122","article-title":"Zika, Chikungunya, and other emerging vector-borne viral diseases","volume":"69","author":"Weaver","year":"2018","journal-title":"Annu. Rev. Med."},{"key":"2025021008405821900_r9","doi-asserted-by":"crossref","first-page":"6104","DOI":"10.1128\/JVI.73.7.6104-6110.1999","article-title":"Dengue virus type 1 nonstructural glycoprotein NS1 is secreted from mammalian cells as a soluble hexamer in a glycosylation-dependent fashion","volume":"73","author":"Flamand","year":"1999","journal-title":"J. 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Immunol."},{"key":"2025021008405821900_r31","doi-asserted-by":"crossref","first-page":"290","DOI":"10.1016\/j.virol.2009.12.010","article-title":"Comprehensive mapping of immunodominant and conserved serotype- and group-specific B-cell epitopes of nonstructural protein 1 from dengue virus type 1","volume":"398","author":"Chen","year":"2010","journal-title":"Virology"},{"key":"2025021008405821900_r32","doi-asserted-by":"crossref","first-page":"e1066","DOI":"10.1002\/cti2.1066","article-title":"Novel differential linear B-cell epitopes to identify Zika and dengue virus infections in patients","volume":"8","author":"Amrun","year":"2019","journal-title":"Clin. Transl. Immunology"},{"key":"2025021008405821900_r33","doi-asserted-by":"crossref","first-page":"1189","DOI":"10.1038\/s41590-018-0210-3","article-title":"The immune response against flaviviruses","volume":"19","author":"Slon Campos","year":"2018","journal-title":"Nat. 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J. Trop. Med. Hyg."},{"key":"2025021008405821900_r39","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1128\/JCM.40.02.376-381.2002","article-title":"Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections","volume":"40","author":"Alcon","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"2025021008405821900_r40","doi-asserted-by":"crossref","first-page":"e94655","DOI":"10.1371\/journal.pone.0094655","article-title":"A meta-analysis of the diagnostic accuracy of two commercial NS1 antigen ELISA tests for early dengue virus detection","volume":"9","author":"da Costa","year":"2014","journal-title":"PLoS One"},{"key":"2025021008405821900_r41","doi-asserted-by":"crossref","first-page":"1292","DOI":"10.1093\/infdis\/jir014","article-title":"Kinetics of plasma viremia and soluble nonstructural protein 1 concentrations in dengue: differential effects according to serotype and immune status","volume":"203","author":"Duyen","year":"2011","journal-title":"J. Infect. Dis."},{"key":"2025021008405821900_r42","doi-asserted-by":"crossref","first-page":"204","DOI":"10.1186\/s12879-017-2306-z","article-title":"Increased sensitivity of NS1 ELISA by heat dissociation in acute dengue 4 cases","volume":"17","author":"Buonora","year":"2017","journal-title":"BMC Infect. Dis."},{"key":"2025021008405821900_r43","doi-asserted-by":"crossref","first-page":"105","DOI":"10.1016\/j.jviromet.2014.02.031","article-title":"A simple heat dissociation method increases significantly the ELISA detection sensitivity of the nonstructural-1 glycoprotein in patients infected with DENV type-4","volume":"204","author":"Lima","year":"2014","journal-title":"J. Virol. Methods"},{"key":"2025021008405821900_r44","doi-asserted-by":"crossref","first-page":"4154","DOI":"10.1128\/JCM.41.9.4154-4159.2003","article-title":"Detection of immune-complex-dissociated nonstructural-1 antigen in patients with acute dengue virus infections","volume":"41","author":"Koraka","year":"2003","journal-title":"J. Clin. 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Dis."},{"key":"2025021008405821900_r55","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1016\/j.diagmicrobio.2007.11.010","article-title":"Evaluation of an NS1 antigen detection for diagnosis of acute dengue infection in patients with acute febrile illness","volume":"60","author":"Lapphra","year":"2008","journal-title":"Diagn. Microbiol. Infect. Dis."},{"key":"2025021008405821900_r56","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1016\/j.ijid.2003.03.003","article-title":"Dengue diagnosis, advances and challenges","volume":"8","author":"Guzm\u00e1n","year":"2004","journal-title":"Int. J. Infect. Dis."},{"key":"2025021008405821900_r57","first-page":"203","article-title":"Application of molecular method to the diagnosis and characterization of a dengue outbreak in Cuba","volume":"18","author":"Rosario","year":"2001","journal-title":"Biotecnol. 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