{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T14:29:03Z","timestamp":1782743343536,"version":"3.54.5"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"the Key-area Research and Development Program of Guangdong Province","award":["2020B1111100010"],"award-info":[{"award-number":["2020B1111100010"]}]},{"name":"the China-Dutch special projects of Guangdong Provincial Hospital of Chinese Medicine","award":["YN2019HL02"],"award-info":[{"award-number":["YN2019HL02"]}]},{"name":"the China-Dutch special projects of Guangdong Provincial Hospital of Chinese Medicine","award":["YN2019HL03"],"award-info":[{"award-number":["YN2019HL03"]}]},{"name":"the Specific Fund of State Key Laboratory of Dampness Syndrome of Chinese Medicine","award":["SZ2021KF16"],"award-info":[{"award-number":["SZ2021KF16"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Gout is a chronic disease caused by monosodium urate crystal deposition. Previous studies have focused on the resident macrophage, infiltrating monocyte, and neutrophil responses to monosodium urate crystal, yet the mechanisms of the potential involvement of other immune cells remain largely unknown. In this study, we enrolled seven gout patients and five age-matched healthy individuals and applied single-cell mass cytometry to study the distribution of immune cell subsets in peripheral blood. To our knowledge, our study reveals the immune cell profiles of gout at different stages for the first time. We identified many immune cell subsets that are dysregulated in gout and promote gouty inflammation, especially those highly expressing CCR4 and OX40 (TNFR superfamily member 4), including CCR4+OX40+ monocytes, CCR4+OX40+CD56high NK cells, CCR4+OX40+CD4+ NK T cells, and CCR4+CD38+CD4+ na\u00efve T cells. Notably, the plasma levels of CCL17 and CCL22, measured by ELISA, increased in the acute phase of gout and declined in the interval. We also found a clue that Th2-type immune responses may participate in gout pathology. Moreover, the subset of granzyme B+ (GZMB+) CD38+ NK cells is positively correlated with serum urea acid level, and another two \u03b3\u03b4T subsets, GZMB+CD161+ \u03b3\u03b4T cells and GZMB+CCR5+ \u03b3\u03b4T cells, are negatively correlated with erythrocyte sedimentation rate. In sum, gouty arthritis is not a disease simply mediated by macrophages; multiple types of immune cell may be involved in the pathogenesis of the disease. Future research needs to shift attention to other immune cell subsets, such as NK cells and T cells, which will facilitate the identification of novel therapeutic targets.<\/jats:p>","DOI":"10.4049\/jimmunol.2200422","type":"journal-article","created":{"date-parts":[[2023,1,27]],"date-time":"2023-01-27T14:22:27Z","timestamp":1674829347000},"page":"745-752","source":"Crossref","is-referenced-by-count":13,"title":["Single-Cell Analysis in Blood Reveals Distinct Immune Cell Profiles in Gouty Arthritis"],"prefix":"10.1093","volume":"210","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1417-416X","authenticated-orcid":false,"given":"Maojie","family":"Wang","sequence":"first","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]},{"name":"Molecular Cancer Research, Center of Molecular Medicine, University Medical Center Utrecht and the Oncode Institute , Utrecht,","place":["the Netherlands"]},{"name":"Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine , Guangzhou,","place":["China"]},{"name":"Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4214-6484","authenticated-orcid":false,"given":"Wenying","family":"Chen","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolin","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0587-8325","authenticated-orcid":false,"given":"Liyan","family":"Mei","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8512-7670","authenticated-orcid":false,"given":"Xiaodong","family":"Wu","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiumin","family":"Chen","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9301-0317","authenticated-orcid":false,"given":"Zhihua","family":"Yang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5770-4269","authenticated-orcid":false,"given":"Kaixin","family":"Gao","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1952-0247","authenticated-orcid":false,"given":"Huanjie","family":"Huang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]},{"name":"Molecular Cancer Research, Center of Molecular Medicine, University Medical Center Utrecht and the Oncode Institute , Utrecht,","place":["the Netherlands"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Runyue","family":"Huang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital, Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]},{"name":"Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine , Guangzhou,","place":["China"]},{"name":"Guangdong-Hong Kong-Macau Joint Lab on Chinese Medicine and Immune Disease Research, Guangzhou University of Chinese Medicine , Guangzhou,","place":["China"]},{"name":"State Key Laboratory of Dampness Syndrome of Chinese Medicine, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine (Guangdong Provincial Hospital of Chinese Medicine) , Guangzhou,","place":["China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,3,15]]},"reference":[{"key":"2025021008013416300_r1","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/s41584-020-0441-1","article-title":"Global epidemiology of gout: prevalence, incidence, treatment patterns and risk factors","volume":"16","author":"Dehlin","year":"2020","journal-title":"Nat. Rev. Rheumatol."},{"key":"2025021008013416300_r2","doi-asserted-by":"crossref","first-page":"69","DOI":"10.1038\/s41572-019-0115-y","article-title":"Gout","volume":"5","author":"Dalbeth","year":"2019","journal-title":"Nat. Rev. Dis. Primers"},{"key":"2025021008013416300_r3","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1038\/nature04516","article-title":"Gout-associated uric acid crystals activate the NALP3 inflammasome","volume":"440","author":"Martinon","year":"2006","journal-title":"Nature"},{"key":"2025021008013416300_r4","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1002\/art.24185","article-title":"Resident macrophages initiating and driving inflammation in a monosodium urate monohydrate crystal-induced murine peritoneal model of acute gout","volume":"60","author":"Martin","year":"2009","journal-title":"Arthritis Rheum."},{"key":"2025021008013416300_r5","doi-asserted-by":"crossref","first-page":"3213","DOI":"10.4049\/jimmunol.138.10.3213","article-title":"Interleukin 1 (IL 1) as a mediator of crystal arthritis. Stimulation of T cell and synovial fibroblast mitogenesis by urate crystal-induced IL 1","volume":"138","author":"Di Giovine","year":"1987","journal-title":"J. Immunol."},{"key":"2025021008013416300_r6","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1002\/art.1780260806","article-title":"Isolation of an interleukin-1-like factor from human joint effusions","volume":"26","author":"Wood","year":"1983","journal-title":"Arthritis Rheum."},{"key":"2025021008013416300_r7","doi-asserted-by":"crossref","first-page":"104351","DOI":"10.1016\/j.phrs.2019.104351","article-title":"IL-17A neutralizing antibody regulates monosodium urate crystal-induced gouty inflammation","volume":"147","author":"Raucci","year":"2019","journal-title":"Pharmacol. Res."},{"key":"2025021008013416300_r8","doi-asserted-by":"crossref","first-page":"789","DOI":"10.4049\/jimmunol.2000319","article-title":"Soluble uric acid is an intrinsic negative regulator of monocyte activation in monosodium urate crystal-induced tissue inflammation","volume":"205","author":"Ma","year":"2020","journal-title":"J. Immunol."},{"key":"2025021008013416300_r9","doi-asserted-by":"crossref","first-page":"1710","DOI":"10.3389\/fimmu.2017.01710","article-title":"Invariant natural killer T cells ameliorate monosodium urate crystal-induced gouty inflammation in mice","volume":"8","author":"Wang","year":"2017","journal-title":"Front. Immunol."},{"key":"2025021008013416300_r10","doi-asserted-by":"crossref","first-page":"2121","DOI":"10.1038\/s41596-018-0016-7","article-title":"Metal-isotope-tagged monoclonal antibodies for high-dimensional mass cytometry","volume":"13","author":"Han","year":"2018","journal-title":"Nat. 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Immunol."}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/210\/6\/745\/61468944\/ji2200422.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/210\/6\/745\/61468944\/ji2200422.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T08:04:28Z","timestamp":1739174668000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/210\/6\/745\/7930437"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,3]]},"references-count":27,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2023,3,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.2200422","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,3]]},"published":{"date-parts":[[2023,3]]}}}