{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:37:14Z","timestamp":1792791434054,"version":"4.5.0"},"reference-count":59,"publisher":"Oxford University Press (OUP)","issue":"10","license":[{"start":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T00:00:00Z","timestamp":1681084800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI123244"],"award-info":[{"award-number":["AI123244"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100015691","name":"HHS | NIH | NIAID | Division of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["AI125516"],"award-info":[{"award-number":["AI125516"]}],"id":[{"id":"10.13039\/100015691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The Brucella abortus double-mutant (\u0394znuA \u0394norD Brucella abortus-lacZ [znBAZ]) was assessed for its protective efficacy after vaccination with a single nasal dose. Superior protection was achieved in znBAZ-vaccinated mice against pulmonary, wild-type B. abortus 2308 challenge when compared with conventional livestock Brucella abortus vaccines, the smooth S19 (smooth B. abortus strain 19 vaccine) and rough RB51 (rough mutant vaccine strain of B. abortus) strains. Nasal znBAZ vaccination reduced splenic and lung colonization by wild-type brucellae by &amp;gt;3\u20134 logs. In contrast, S19 reduced lung colonization by only 32-fold, and RB51 failed to reduce colonization. One profound attribute of znBAZ vaccination was the &amp;gt;3-fold increase in pulmonary CD8+ T cells when compared with other vaccinated groups. S19 vaccination increased only CD4+ T cells. All vaccines induced IFN-\u03b3 and TNF-\u03b1 production by CD4+ T cells, but only znBAZ vaccination enhanced the recruitment of polyfunctional CD8+ T cells, by &amp;gt;100-fold. IL-17 by both CD4+ and CD8+ T cells was also induced by subsequent znBAZ vaccination. These results demonstrate that, in addition to achieving protective immunity by CD4+ T cells, CD8+ T cells, specifically resident memory T cells, also confer protection against brucellosis. The protection obtained by znBAZ vaccination was attributed to IFN-\u03b3\u2013producing CD8+ T cells, because depletion of CD8+ T cells throughout vaccination and challenge phases abrogated protection. The stimulation of only CD4+ T cells by RB51- and S19-vaccinated mice proved insufficient in protecting against pulmonary B. abortus 2308 challenge. Thus, nasal znBAZ vaccination offers an alternative means to elicit protection against brucellosis.<\/jats:p>","DOI":"10.4049\/jimmunol.2300071","type":"journal-article","created":{"date-parts":[[2023,4,10]],"date-time":"2023-04-10T13:27:10Z","timestamp":1681133230000},"page":"1576-1588","source":"Crossref","is-referenced-by-count":3,"title":["A Single Nasal Dose Vaccination with a <i>Brucella abortus<\/i> Mutant Potently Protects against Pulmonary Infection"],"prefix":"10.1093","volume":"210","author":[{"given":"Hongbin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Immunology, University of Florida , Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Beata","family":"Clapp","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology, University of Florida , Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carol","family":"Hoffman","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology, University of Florida , Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinghong","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology, University of Florida , Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6572-6916","authenticated-orcid":false,"given":"David W","family":"Pascual","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Immunology, University of Florida , Gainesville, FL"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,4,10]]},"reference":[{"key":"2025021008052607900_r1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1146\/annurev-animal-021815-111326","article-title":"Brucella spp. Virulence factors and immunity","volume":"4","author":"Byndloss","year":"2016","journal-title":"Annu. Rev. Anim. Biosci."},{"key":"2025021008052607900_r2","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1016\/S1473-3099(06)70382-6","article-title":"The new global map of human brucellosis","volume":"6","author":"Pappas","year":"2006","journal-title":"Lancet Infect. Dis."},{"key":"2025021008052607900_r3","doi-asserted-by":"crossref","first-page":"249","DOI":"10.20506\/rst.32.1.2197","article-title":"Economics of brucellosis impact and control in low-income countries","volume":"32","author":"McDermott","year":"2013","journal-title":"Rev. Sci. Tech."},{"key":"2025021008052607900_r4","doi-asserted-by":"crossref","first-page":"1505","DOI":"10.1016\/j.ajpath.2015.03.003","article-title":"Pathogenesis and immunobiology of brucellosis: review of Brucella-host interactions","volume":"185","author":"de Figueiredo","year":"2015","journal-title":"Am. J. Pathol."},{"key":"2025021008052607900_r5","doi-asserted-by":"crossref","first-page":"213","DOI":"10.3201\/eid0302.970219","article-title":"Brucellosis: an overview","volume":"3","author":"Corbel","year":"1997","journal-title":"Emerg. Infect. Dis."},{"key":"2025021008052607900_r6","doi-asserted-by":"crossref","first-page":"1018165","DOI":"10.3389\/fmicb.2022.1018165","article-title":"Activation of mucosal immunity as a novel therapeutic strategy for combating brucellosis","volume":"13","author":"Pascual","year":"2022","journal-title":"Front. Microbiol."},{"key":"2025021008052607900_r7","doi-asserted-by":"crossref","first-page":"60","DOI":"10.1007\/s11515-012-1196-0","article-title":"Progress in Brucella vaccine development","volume":"8","author":"Yang","year":"2013","journal-title":"Front. Biol. (Beijing)"},{"key":"2025021008052607900_r8","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1186\/s13567-015-0199-7","article-title":"Recent advances in Brucella abortus vaccines","volume":"46","author":"Dorneles","year":"2015","journal-title":"Vet. Res. (Faisalabad)"},{"key":"2025021008052607900_r9","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1007\/s00430-009-0123-8","article-title":"Survival of the fittest: how Brucella strains adapt to their intracellular niche in the host","volume":"198","author":"Roop","year":"2009","journal-title":"Med. Microbiol. Immunol. (Berl.)"},{"key":"2025021008052607900_r10","first-page":"117","volume-title":"Mucosal Vaccines","author":"Lycke","year":"2019","edition":"2nd"},{"key":"2025021008052607900_r11","doi-asserted-by":"crossref","first-page":"517","DOI":"10.1093\/intimm\/dxu063","article-title":"Challenges in mucosal vaccines for the control of infectious diseases","volume":"26","author":"Azegami","year":"2014","journal-title":"Int. Immunol."},{"key":"2025021008052607900_r12","doi-asserted-by":"crossref","first-page":"394","DOI":"10.1016\/j.jconrel.2016.02.014","article-title":"Mucosal vaccine delivery: current state and a pediatric perspective","volume":"240","author":"Shakya","year":"2016","journal-title":"J. Control. Release"},{"key":"2025021008052607900_r13","doi-asserted-by":"crossref","first-page":"193","DOI":"10.1111\/j.1574-695X.1996.tb00136.x","article-title":"Comparison of T cell cytokines in resistant and susceptible mice infected with virulent Brucella abortus strain 2308","volume":"16","author":"Fernandes","year":"1996","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"2025021008052607900_r14","doi-asserted-by":"crossref","first-page":"3330","DOI":"10.4049\/jimmunol.143.10.3330","article-title":"Temporal development of protective cell-mediated and humoral immunity in BALB\/c mice infected with Brucella abortus","volume":"143","author":"Araya","year":"1989","journal-title":"J. Immunol."},{"key":"2025021008052607900_r15","doi-asserted-by":"crossref","first-page":"4165","DOI":"10.1128\/IAI.05080-11","article-title":"Protective live oral brucellosis vaccines stimulate Th1 and th17 cell responses","volume":"79","author":"Clapp","year":"2011","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r16","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1006\/cimm.1996.0241","article-title":"Recombinant Brucella abortus proteins that induce proliferation and gamma-interferon secretion by CD4+ T cells from Brucella-vaccinated mice and delayed-type hypersensitivity in sensitized guinea pigs","volume":"172","author":"Oliveira","year":"1996","journal-title":"Cell. Immunol."},{"key":"2025021008052607900_r17","doi-asserted-by":"crossref","first-page":"4271","DOI":"10.1128\/IAI.00761-12","article-title":"Crucial role of gamma interferon-producing CD4+ Th1 cells but dispensable function of CD8+ T cell, B cell, Th2, and Th17 responses in the control of Brucella melitensis infection in mice","volume":"80","author":"Vitry","year":"2012","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r18","doi-asserted-by":"crossref","first-page":"686919","DOI":"10.1155\/2013\/686919","article-title":"CD8 knockout mice are protected from challenge by vaccination with WR201, a live attenuated mutant of Brucella melitensis","volume":"2013","author":"Yingst","year":"2013","journal-title":"Clin. Dev. Immunol."},{"key":"2025021008052607900_r19","doi-asserted-by":"crossref","first-page":"3740","DOI":"10.4049\/jimmunol.1302561","article-title":"Humoral immunity and CD4+ Th1 cells are both necessary for a fully protective immune response upon secondary infection with Brucella melitensis","volume":"192","author":"Vitry","year":"2014","journal-title":"J. Immunol."},{"key":"2025021008052607900_r20","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1038\/icb.2016.5","article-title":"Nasal vaccination stimulates CD8(+) T cells for potent protection against mucosal Brucella melitensis challenge","volume":"94","author":"Clapp","year":"2016","journal-title":"Immunol. Cell Biol."},{"key":"2025021008052607900_r21","doi-asserted-by":"crossref","first-page":"2551","DOI":"10.1002\/eji.1830250922","article-title":"CD8+ type 1 CD44hi CD45 RBlo T lymphocytes control intracellular Brucella abortus infection as demonstrated in major histocompatibility complex class I- and class II-deficient mice","volume":"25","author":"Oliveira","year":"1995","journal-title":"Eur. J. Immunol."},{"key":"2025021008052607900_r22","doi-asserted-by":"crossref","first-page":"2408","DOI":"10.1016\/j.vaccine.2009.12.076","article-title":"An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8+ cytotoxic T cell response","volume":"28","author":"Hu","year":"2010","journal-title":"Vaccine"},{"key":"2025021008052607900_r23","doi-asserted-by":"crossref","first-page":"4014","DOI":"10.4049\/jimmunol.1501188","article-title":"A Brucella spp. protease inhibitor limits antigen lysosomal proteolysis, increases cross-presentation, and enhances CD8+ T cell responses","volume":"196","author":"Coria","year":"2016","journal-title":"J. Immunol."},{"key":"2025021008052607900_r24","doi-asserted-by":"crossref","first-page":"e0136696","DOI":"10.1371\/journal.pone.0136696","article-title":"Immune response of calves vaccinated with Brucella abortus S19 or RB51 and revaccinated with RB51","volume":"10","author":"Dorneles","year":"2015","journal-title":"PLoS One"},{"key":"2025021008052607900_r25","doi-asserted-by":"crossref","first-page":"1153","DOI":"10.2460\/ajvr.1996.57.08.1153","article-title":"Effects of age at vaccination on efficacy of Brucella abortus strain RB51 to protect cattle against brucellosis","volume":"57","author":"Cheville","year":"1996","journal-title":"Am. J. Vet. Res."},{"key":"2025021008052607900_r26","first-page":"21","article-title":"Brucellosis: past, present and future","volume":"31","author":"Nicoletti","year":"2010","journal-title":"Prilozi"},{"key":"2025021008052607900_r27","doi-asserted-by":"crossref","first-page":"171","DOI":"10.1016\/0378-1135(91)90091-S","article-title":"Biological properties of RB51; a stable rough strain of Brucella abortus","volume":"28","author":"Schurig","year":"1991","journal-title":"Vet. Microbiol."},{"key":"2025021008052607900_r28","doi-asserted-by":"crossref","first-page":"677","DOI":"10.2460\/ajvr.1996.57.05.677","article-title":"Protection of BALB\/c mice against homologous and heterologous species of Brucella by rough strain vaccines derived from Brucella melitensis and Brucella suis biovar 4","volume":"57","author":"Winter","year":"1996","journal-title":"Am. J. Vet. Res."},{"key":"2025021008052607900_r29","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1128\/IAI.66.3.1008-1016.1998","article-title":"Transposon-derived Brucella abortus rough mutants are attenuated and exhibit reduced intracellular survival","volume":"66","author":"Allen","year":"1998","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r30","doi-asserted-by":"crossref","first-page":"6034","DOI":"10.1016\/j.vaccine.2014.08.060","article-title":"T lymphocytes subsets and cytokine pattern induced by vaccination against bovine brucellosis employing S19 calfhood vaccination and adult RB51 revaccination","volume":"32","author":"Dorneles","year":"2014","journal-title":"Vaccine"},{"key":"2025021008052607900_r31","doi-asserted-by":"crossref","first-page":"6541","DOI":"10.1128\/IAI.69.10.6541-6544.2001","article-title":"Mouse cytokine profiles associated with Brucella abortus RB51 vaccination or B. abortus 2308 infection","volume":"69","author":"Pasquali","year":"2001","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r32","doi-asserted-by":"crossref","first-page":"5502","DOI":"10.1128\/IAI.69.9.5502-5508.2001","article-title":"Induction of specific cytotoxic lymphocytes in mice vaccinated with Brucella abortus RB51","volume":"69","author":"He","year":"2001","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r33","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/j.1348-0421.2006.tb03805.x","article-title":"Vaccination with live Escherichia coli expressing Brucella abortus Cu\/Zn superoxide-dismutase: II. Induction of specific CD8+ cytotoxic lymphocytes and sensitized CD4+ IFN-\u03b3-producing cell","volume":"50","author":"Andrews","year":"2006","journal-title":"Microbiol. Immunol."},{"key":"2025021008052607900_r34","doi-asserted-by":"crossref","first-page":"5290","DOI":"10.1016\/j.vaccine.2016.09.004","article-title":"Vaccination with a \u0394norD \u0394znuA Brucella abortus mutant confers potent protection against virulent challenge","volume":"34","author":"Yang","year":"2016","journal-title":"Vaccine"},{"key":"2025021008052607900_r35","doi-asserted-by":"crossref","first-page":"3874","DOI":"10.1128\/IAI.01957-05","article-title":"Deletion of znuA virulence factor attenuates Brucella abortus and confers protection against wild-type challenge","volume":"74","author":"Yang","year":"2006","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r36","doi-asserted-by":"crossref","first-page":"e1008176","DOI":"10.1371\/journal.ppat.1008176","article-title":"Targeting resident memory T cell immunity culminates in pulmonary and systemic protection against Brucella infection","volume":"16","author":"Wang","year":"2020","journal-title":"PLoS Pathog."},{"key":"2025021008052607900_r37","doi-asserted-by":"crossref","first-page":"2749","DOI":"10.1016\/j.vaccine.2011.01.090","article-title":"Efficacy of vaccination strategies against intranasal challenge with Brucella abortus in BALB\/c mice","volume":"29","author":"Surendran","year":"2011","journal-title":"Vaccine"},{"key":"2025021008052607900_r38","doi-asserted-by":"crossref","first-page":"4103","DOI":"10.1016\/j.vaccine.2013.06.078","article-title":"Protection to respiratory challenge of Brucella abortus strain 2308 in the lung","volume":"31","author":"Surendran","year":"2013","journal-title":"Vaccine"},{"key":"2025021008052607900_r39","doi-asserted-by":"crossref","first-page":"382","DOI":"10.1016\/j.vetmic.2014.11.021","article-title":"The protoxin Cry1Ac of Bacillus thuringiensis improves the protection conferred by intranasal immunization with Brucella abortus RB51 in a mouse model","volume":"175","author":"Gonz\u00e1lez-Gonz\u00e1lez","year":"2015","journal-title":"Vet. Microbiol."},{"key":"2025021008052607900_r40","doi-asserted-by":"crossref","first-page":"e107180","DOI":"10.1371\/journal.pone.0107180","article-title":"Oral immunization of mice with gamma-irradiated Brucella neotomae induces protection against intraperitoneal and intranasal challenge with virulent B. abortus 2308","volume":"9","author":"Dabral","year":"2014","journal-title":"PLoS One"},{"key":"2025021008052607900_r41","doi-asserted-by":"crossref","first-page":"4031","DOI":"10.1128\/IAI.72.7.4031-4039.2004","article-title":"Oral vaccination with Brucella melitensis WR201 protects mice against intranasal challenge with virulent Brucella melitensis 16M","volume":"72","author":"Izadjoo","year":"2004","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r42","doi-asserted-by":"crossref","first-page":"1447","DOI":"10.4049\/jimmunol.161.3.1447","article-title":"Control of IL-12 and IFN-\u03b3 production in response to live or dead bacteria by TNF and other factors","volume":"161","author":"Zhan","year":"1998","journal-title":"J. Immunol."},{"key":"2025021008052607900_r43","doi-asserted-by":"crossref","first-page":"eaas9673","DOI":"10.1126\/sciimmunol.aas9673","article-title":"Location, location, location: tissue resident memory T cells in mice and humans","volume":"4","author":"Szabo","year":"2019","journal-title":"Sci. Immunol."},{"key":"2025021008052607900_r44","doi-asserted-by":"crossref","first-page":"545","DOI":"10.1038\/mi.2016.48","article-title":"Intranasal administration of RSV antigen-expressing MCMV elicits robust tissue-resident effector and effector memory CD8+ T cells in the lung","volume":"10","author":"Morabito","year":"2017","journal-title":"Mucosal Immunol."},{"key":"2025021008052607900_r45","doi-asserted-by":"crossref","first-page":"406","DOI":"10.1038\/ni.3108","article-title":"Proinflammatory microenvironments within the intestine regulate the differentiation of tissue-resident CD8+ T cells responding to infection","volume":"16","author":"Bergsbaken","year":"2015","journal-title":"Nat. Immunol."},{"key":"2025021008052607900_r46","doi-asserted-by":"crossref","first-page":"687","DOI":"10.1016\/j.immuni.2013.08.019","article-title":"Transforming growth factor-\u03b2 signaling controls the formation and maintenance of gut-resident memory T cells by regulating migration and retention","volume":"39","author":"Zhang","year":"2013","journal-title":"Immunity"},{"key":"2025021008052607900_r47","doi-asserted-by":"crossref","first-page":"551","DOI":"10.1056\/NEJMra1204186","article-title":"Designing tomorrow\u2019s vaccines","volume":"368","author":"Nabel","year":"2013","journal-title":"N. Engl. J. Med."},{"key":"2025021008052607900_r48","doi-asserted-by":"crossref","first-page":"3659","DOI":"10.1016\/j.vaccine.2015.05.057","article-title":"Immune response triggered by Brucella abortus following infection or vaccination","volume":"33","author":"Dorneles","year":"2015","journal-title":"Vaccine"},{"key":"2025021008052607900_r49","doi-asserted-by":"crossref","first-page":"5877","DOI":"10.1128\/IAI.67.11.5877-5884.1999","article-title":"Protection of mice against brucellosis by vaccination with Brucella melitensis WR201(16MDeltapurEK)","volume":"67","author":"Hoover","year":"1999","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r50","doi-asserted-by":"crossref","first-page":"4923","DOI":"10.1128\/IAI.00451-07","article-title":"Aerosol infection of BALB\/c mice with Brucella melitensis and Brucella abortus and protective efficacy against aerosol challenge","volume":"75","author":"Kahl-McDonagh","year":"2007","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r51","doi-asserted-by":"crossref","first-page":"29","DOI":"10.1186\/1297-9716-43-29","article-title":"What have we learned from brucellosis in the mouse model?","volume":"43","author":"Grill\u00f3","year":"2012","journal-title":"Vet. Res. (Faisalabad)"},{"key":"2025021008052607900_r52","doi-asserted-by":"crossref","first-page":"e74729","DOI":"10.1371\/journal.pone.0074729","article-title":"Lack of endogenous IL-10 enhances production of proinflammatory cytokines and leads to Brucella abortus clearance in mice","volume":"8","author":"Corsetti","year":"2013","journal-title":"PLoS One"},{"key":"2025021008052607900_r53","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1016\/j.micinf.2018.12.004","article-title":"Inhibition of antigen presentation by Brucella: many more than many ways","volume":"21","author":"Barrionuevo","year":"2019","journal-title":"Microbes Infect."},{"key":"2025021008052607900_r54","doi-asserted-by":"crossref","first-page":"4759","DOI":"10.1128\/IAI.01184-15","article-title":"CD8+ T cell exhaustion, suppressed gamma interferon production, and delayed memory response induced by chronic Brucella melitensis infection","volume":"83","author":"Durward-Diioia","year":"2015","journal-title":"Infect. Immun."},{"key":"2025021008052607900_r55","doi-asserted-by":"crossref","first-page":"3780","DOI":"10.4049\/jimmunol.1502265","article-title":"Identification of immune effectors essential to the control of primary and secondary intranasal infection with Brucella melitensis in mice","volume":"196","author":"Hanot Mambres","year":"2016","journal-title":"J. Immunol."},{"key":"2025021008052607900_r56","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1046\/j.1365-2567.2001.01258.x","article-title":"Interferon-gamma is crucial for surviving a Brucella abortus infection in both resistant C57BL\/6 and susceptible BALB\/c mice","volume":"103","author":"Murphy","year":"2001","journal-title":"Immunology"},{"key":"2025021008052607900_r57","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1038\/ni1449","article-title":"IL-23 and IL-17 in the establishment of protective pulmonary CD4+ T cell responses after vaccination and during Mycobacterium tuberculosis challenge","volume":"8","author":"Khader","year":"2007","journal-title":"Nat. Immunol."},{"key":"2025021008052607900_r58","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1111\/imr.12183","article-title":"Directing traffic: IL-17 and IL-22 coordinate pulmonary immune defense","volume":"260","author":"McAleer","year":"2014","journal-title":"Immunol. Rev."},{"key":"2025021008052607900_r59","doi-asserted-by":"crossref","first-page":"e21978","DOI":"10.1371\/journal.pone.0021978","article-title":"Murine and bovine \u03b3\u03b4 T cells enhance innate immunity against Brucella abortus infections","volume":"6","author":"Skyberg","year":"2011","journal-title":"PLoS One"}],"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/210\/10\/1576\/61468807\/ji2300071.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/210\/10\/1576\/61468807\/ji2300071.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T08:11:15Z","timestamp":1739175075000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/210\/10\/1576\/7930410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,10]]},"references-count":59,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2023,5,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.2300071","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2023,5]]},"published":{"date-parts":[[2023,4,10]]}}}