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Exposure to allergens produced by a variety of otherwise harmless microbes, including fungi, predisposes individuals to immunopathologic disease upon subsequent encounters with allergen. We developed a mouse model that employs a purified protease produced by Aspergillus (Asp f 13) to investigate the contributions of CD4+ Th cells to recurrent lung inflammation. Notably, memory CD4+ T cells enhanced the eosinophil response of sensitized\/rechallenged animals. In addition, memory CD4+ T cells maintained allergenic features, including expression of GATA-binding protein 3 and IL-5. Th2 memory T cells persisted in the peribronchiolar interstitium of the lung and expressed markers of tissue residence, such as CD69, CCR8, and IL-33R. Lastly, we identified a peptide epitope contained within Asp f 13 and generated a peptide\u2013MHC class II tetramer. Using these tools, we further demonstrated the durability and exquisite sensitivity of memory T cells in promoting lung eosinophilia. Our data highlight important features of memory T cells that strengthen the notion that memory T cells are principal drivers of eosinophilic disease in murine models of allergic sensitization and episodic airway inflammation.<\/jats:p>","DOI":"10.4049\/jimmunol.2300694","type":"journal-article","created":{"date-parts":[[2024,3,15]],"date-time":"2024-03-15T13:57:58Z","timestamp":1710511078000},"page":"1420-1427","source":"Crossref","is-referenced-by-count":3,"title":["Sensitization with Fungal Protease Allergen Establishes Long-Lived, Allergenic Th Cell Memory in the Lung"],"prefix":"10.1093","volume":"212","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1311-0034","authenticated-orcid":false,"given":"Abigail","family":"Shapiro","sequence":"first","affiliation":[{"name":"Center for Immunity and Inflammation, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"},{"name":"Department of Medicine, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicolas W S","family":"Caballes","sequence":"additional","affiliation":[{"name":"Center for Immunity and Inflammation, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"},{"name":"Department of Medicine, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebecca N","family":"Vera","sequence":"additional","affiliation":[{"name":"Center for Immunity and Inflammation, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"},{"name":"Department of Medicine, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce S","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, University of Wisconsin\u2013Madison , Madison, WI"},{"name":"Department of Medicine, University of Wisconsin\u2013Madison , Madison, WI"},{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin\u2013Madison , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2342-0094","authenticated-orcid":false,"given":"Paul J","family":"Brennan","sequence":"additional","affiliation":[{"name":"Center for Immunity and Inflammation, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"},{"name":"Department of Medicine, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen-Fei","family":"Wu","sequence":"additional","affiliation":[{"name":"Center for Immunity and Inflammation, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"},{"name":"Department of Medicine, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darin L","family":"Wiesner","sequence":"additional","affiliation":[{"name":"Center for Immunity and Inflammation, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"},{"name":"Department of Medicine, NJ Medical School, Rutgers\u2013The State University of New Jersey , Newark, NJ"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,3,15]]},"reference":[{"key":"2025020405485349900_r1","volume-title":"Asthma Facts: CDC's National Asthma Control Program Grantees.","author":"Centers for Disease Control and Prevention","year":"2013"},{"key":"2025020405485349900_r2","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1513\/AnnalsATS.201703-259OC","article-title":"The economic burden of asthma in the United States, 2008-2013","volume":"15","author":"Nurmagambetov","year":"2018","journal-title":"Ann. 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