{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T01:30:25Z","timestamp":1776821425796,"version":"3.51.2"},"reference-count":61,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T00:00:00Z","timestamp":1729814400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003958","name":"NWO | AES | Stichting voor de Technische Wetenschappen","doi-asserted-by":"publisher","award":["16442"],"award-info":[{"award-number":["16442"]}],"id":[{"id":"10.13039\/501100003958","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000781","name":"EC | European Research Council","doi-asserted-by":"publisher","award":["101001937"],"award-info":[{"award-number":["101001937"]}],"id":[{"id":"10.13039\/501100000781","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Staphylococcus aureus is the major cause of healthcare-associated infections, including life-threatening conditions as bacteremia, endocarditis, and implant-associated infections. Despite adequate antibiotic treatment, the mortality of S. aureus bacteremia remains high. This calls for different strategies to treat this infection. In past years, sequencing of Ab repertoires from individuals previously exposed to a pathogen emerged as a successful method to discover novel therapeutic monoclonal Abs and understand circulating B cell diversity during infection. In this paper, we collected peripheral blood from 17 S. aureus bacteremia patients to study circulating plasmablast responses. Using single-cell transcriptome gene expression combined with sequencing of variable heavy and light Ig genes, we retrieved sequences from &amp;gt;400 plasmablasts revealing a high diversity with &amp;gt;300 unique variable heavy and light sequences. More than 200 variable sequences were synthesized to produce recombinant IgGs that were analyzed for binding to S. aureus whole bacterial cells. This revealed four novel monoclonal Abs that could specifically bind to the surface of S. aureus in the absence of Ig-binding surface SpA. Interestingly, three of four mAbs showed cross-reactivity with Staphylococcus epidermidis. Target identification revealed that the S. aureus\u2013specific mAb BC153 targets wall teichoic acid, whereas cross-reactive mAbs BC019, BC020, and BC021 target lipoteichoic acid. All mAbs could induce Fc-dependent phagocytosis of staphylococci by human neutrophils. Altogether, we characterize the active B cell responses to S. aureus in infected patients and identify four functional mAbs against the S. aureus surface, of which three cross-react with S. epidermidis.<\/jats:p>","DOI":"10.4049\/jimmunol.2300858","type":"journal-article","created":{"date-parts":[[2024,10,25]],"date-time":"2024-10-25T12:53:12Z","timestamp":1729860792000},"page":"1644-1655","source":"Crossref","is-referenced-by-count":4,"title":["Single-cell Sequencing of Circulating Human Plasmablasts during <i>Staphylococcus aureus<\/i> Bacteremia"],"prefix":"10.1093","volume":"213","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6212-002X","authenticated-orcid":false,"given":"Priscilla F","family":"Kerkman","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2289-3378","authenticated-orcid":false,"given":"Lisanne","family":"de Vor","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5873-0474","authenticated-orcid":false,"given":"Thomas W","family":"van der Vaart","sequence":"additional","affiliation":[{"name":"Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]},{"name":"Amsterdam Institute for Infection and Immunity, Infectious Diseases , Amsterdam ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thijs","family":"ten Doesschate","sequence":"additional","affiliation":[{"name":"Julius Center for Health Sciences and Primary Care, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]},{"name":"Department of Internal Medicine, Jeroen Bosch Hospital , Den Bosch ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4325-1382","authenticated-orcid":false,"given":"Remy M","family":"Muts","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8983-2855","authenticated-orcid":false,"given":"Jamie S","family":"Depelteau","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3819-3349","authenticated-orcid":false,"given":"Lisette M","family":"Scheepmaker","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2289-3412","authenticated-orcid":false,"given":"Maartje","family":"Ruyken","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carla J C","family":"de Haas","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Piet C","family":"Aerts","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6836-675X","authenticated-orcid":false,"given":"Renoud J","family":"Marijnissen","sequence":"additional","affiliation":[{"name":"Genmab , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9738-9926","authenticated-orcid":false,"given":"Janine","family":"Schuurman","sequence":"additional","affiliation":[{"name":"Genmab , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank J","family":"Beurskens","sequence":"additional","affiliation":[{"name":"Genmab , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7844-4312","authenticated-orcid":false,"given":"Andrea","family":"Gorlani","sequence":"additional","affiliation":[{"name":"Genmab , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6450-277X","authenticated-orcid":false,"given":"Bart W","family":"Bardoel","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4102-0377","authenticated-orcid":false,"given":"Suzan H M","family":"Rooijakkers","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University Medical Center Utrecht, Utrecht University , Utrecht ,","place":["the Netherlands"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,10,25]]},"reference":[{"key":"2025020609300380000_r1","doi-asserted-by":"crossref","first-page":"178","DOI":"10.3389\/fcimb.2014.00178","article-title":"Staphylococcus aureus biofilms: recent developments in biofilm dispersal","volume":"4","author":"Lister","year":"2014","journal-title":"Front. 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