{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T04:37:49Z","timestamp":1789619869469,"version":"build-2803163510"},"reference-count":49,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM150893"],"award-info":[{"award-number":["R35GM150893"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["R35GM118027"],"award-info":[{"award-number":["R35GM118027"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000092","name":"HHS | NIH | U.S. National Library of Medicine","doi-asserted-by":"publisher","award":["NLM5T15LM007359"],"award-info":[{"award-number":["NLM5T15LM007359"]}],"id":[{"id":"10.13039\/100000092","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100002570","name":"American Association of Immunologists","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100002570","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neutrophils accumulate early in tissue injury. However, the cellular and functional heterogeneity of neutrophils during homeostasis and in response to tissue damage remains unclear. In this study, we use larval zebrafish to understand neutrophil responses to thermal injury. Single-cell transcriptional mapping of myeloid cells during a 3-d time course in burn and control larvae revealed distinct neutrophil subsets and their cell\u2013cell interactions with macrophages across time and conditions. The trajectory formed by three zebrafish neutrophil subsets resembles human neutrophil maturation, with varying transition patterns between conditions. Through ligand\u2013receptor cell\u2013cell interaction analysis, we found that neutrophils communicate more in burns in a pathway and temporal manner. Finally, we identified the correlation between zebrafish myeloid signatures and human burn severity, establishing GPR84+ neutrophils as a potential marker of early innate immune response in burns. This work builds a comparative single-cell transcriptomic framework to identify neutrophil markers of tissue damage using model organisms.<\/jats:p>","DOI":"10.4049\/jimmunol.2400149","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T09:54:50Z","timestamp":1719395690000},"page":"469-480","source":"Crossref","is-referenced-by-count":7,"title":["Single-cell Transcriptional Landscape of Temporal Neutrophil Response to Burn Wound in Larval Zebrafish"],"prefix":"10.1093","volume":"213","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6086-6434","authenticated-orcid":false,"given":"Yiran","family":"Hou","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1998-5324","authenticated-orcid":false,"given":"Parth","family":"Khatri","sequence":"additional","affiliation":[{"name":"McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin School of Medicine and Public Health , Madison, WI"},{"name":"Department of Biostatistics and Medical Informatics, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4242-6427","authenticated-orcid":false,"given":"Julie","family":"Rindy","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zachery","family":"Schultz","sequence":"additional","affiliation":[{"name":"McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8383-4283","authenticated-orcid":false,"given":"Anqi","family":"Gao","sequence":"additional","affiliation":[{"name":"McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-1172-0900","authenticated-orcid":false,"given":"Zhili","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health , Madison, WI"},{"name":"McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1181-7481","authenticated-orcid":false,"given":"Angela L F","family":"Gibson","sequence":"additional","affiliation":[{"name":"Department of Surgery, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anna","family":"Huttenlocher","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health , Madison, WI"},{"name":"Department of Pediatrics, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3307-1126","authenticated-orcid":false,"given":"Huy Q","family":"Dinh","sequence":"additional","affiliation":[{"name":"McArdle Laboratory for Cancer Research, Department of Oncology, University of Wisconsin School of Medicine and Public Health , Madison, WI"},{"name":"Department of Biostatistics and Medical Informatics, University of Wisconsin School of Medicine and Public Health , Madison, WI"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"2025012305131183300_r1","doi-asserted-by":"crossref","first-page":"1851","DOI":"10.1016\/j.burns.2021.02.004","article-title":"Investigation and assessment of neutrophil dysfunction early after severe burn injury","volume":"47","author":"Yang","year":"2021","journal-title":"Burns"},{"key":"2025012305131183300_r2","doi-asserted-by":"crossref","first-page":"1654","DOI":"10.1038\/s41598-022-05768-w","article-title":"Severity of thermal burn injury is associated with systemic neutrophil activation","volume":"12","author":"Laggner","year":"2022","journal-title":"Sci. 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