{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T17:18:12Z","timestamp":1787505492673,"version":"build-2736575974"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T00:00:00Z","timestamp":1725580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001843","name":"DST | Science and Engineering Research Board","doi-asserted-by":"publisher","award":["EEQ\/2021\/0594"],"award-info":[{"award-number":["EEQ\/2021\/0594"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["09\/025(0243)\/2018-EMR-I"],"award-info":[{"award-number":["09\/025(0243)\/2018-EMR-I"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001412","name":"Council of Scientific and Industrial Research, India","doi-asserted-by":"publisher","award":["09\/0025(16530)\/2023-EMR-I"],"award-info":[{"award-number":["09\/0025(16530)\/2023-EMR-I"]}],"id":[{"id":"10.13039\/501100001412","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Dendritic cell (DC)\u2013based vaccines are promising immunotherapies for cancer. Although DC-based therapies are known to activate tumor-specific T cells, the interplay between DCs and NK cells in this setting is not fully understood. In this study, we demonstrated a novel TLR7\/ mitochondrial reactive oxygen species (mROS)\/IL-12 axis that drives potent NK cell responses against tumors. We showed that TLR7 activation by imiquimod in peripheral blood monocyte-derived CD11c+ DCs triggered mROS production, leading to enhanced IL-12 secretion and subsequent NK cell activation, as evidenced by increased IFN-\u03b3 production and tumor cell cytotoxicity. Notably, mROS neutralization abrogates NK cell\u2013mediated tumor cell lysis, and TLR7-mediated DC activation of NK cells occurs independently of MyD88, suggesting involvement of the noncanonical NF-\u03baB pathway. Our findings provide a rationale for targeting the TLR7\/mROS\/IL-12 axis to enhance the efficacy of DC-based cancer immunotherapy.<\/jats:p>","DOI":"10.4049\/jimmunol.2400340","type":"journal-article","created":{"date-parts":[[2024,9,6]],"date-time":"2024-09-06T09:32:46Z","timestamp":1725615166000},"page":"1255-1263","source":"Crossref","is-referenced-by-count":7,"title":["TLR7-Induced Mitochondrial Reactive Oxygen Species Production in Monocyte-derived Dendritic Cells Drives IL-12\u2013Dependent NK Cell Activation and Enhances Antitumor Immunity"],"prefix":"10.1093","volume":"213","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4001-0484","authenticated-orcid":false,"given":"Ankush","family":"Paladhi","sequence":"first","affiliation":[{"name":"Cellular Immunology Laboratory, Department of Zoology, University of Burdwan , Bardhaman ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2181-476X","authenticated-orcid":false,"given":"Samrat","family":"Daripa","sequence":"additional","affiliation":[{"name":"Cellular Immunology Laboratory, Department of Zoology, University of Burdwan , Bardhaman ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arghya","family":"Nath","sequence":"additional","affiliation":[{"name":"ICMR-DHR-VRDL, Burdwan Medical College and Hospital , Bardhaman ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6113-4721","authenticated-orcid":false,"given":"Sumit Kumar","family":"Hira","sequence":"additional","affiliation":[{"name":"Cellular Immunology Laboratory, Department of Zoology, University of Burdwan , Bardhaman ,","place":["India"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,9,6]]},"reference":[{"key":"2025020609274943000_r1","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1016\/j.jcyt.2015.01.006","article-title":"Combined immunotherapy with whole tumor lysate\u2013pulsed interleukin-15\u2013activated dendritic cells and cucurbitacin I promotes strong CD8+ T-cell responses and cures highly aggressive lymphoma","volume":"17","author":"Hira","year":"2015","journal-title":"Cytotherapy"},{"key":"2025020609274943000_r2","doi-asserted-by":"crossref","first-page":"101623","DOI":"10.1016\/j.isci.2020.101623","article-title":"Galunisertib drives Treg fragility and promotes dendritic cell-mediated immunity against experimental lymphoma","volume":"23","author":"Hira","year":"2020","journal-title":"iScience"},{"key":"2025020609274943000_r3","doi-asserted-by":"crossref","first-page":"480","DOI":"10.1038\/nature10673","article-title":"Cancer immunotherapy comes of age","volume":"480","author":"Mellman","year":"2011","journal-title":"Nature"},{"key":"2025020609274943000_r4","doi-asserted-by":"crossref","first-page":"1178","DOI":"10.1038\/s41591-018-0085-8","article-title":"A natural killer\u2013dendritic cell axis defines checkpoint therapy\u2013responsive tumor microenvironments","volume":"24","author":"Barry","year":"2018","journal-title":"Nat. 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