{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,28]],"date-time":"2026-01-28T23:56:35Z","timestamp":1769644595309,"version":"3.49.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2010,4,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>ICOS ligand (ICOSL) plays an important role in controlling specific aspects of T cell activation, differentiation, and function. Th1-type immune responses have been shown to be critical in host defense against chlamydial infections. To assess the role of ICOSL\u2013ICOS interaction in host defense against chlamydial infection, we compared the immune responses and pathological reactions in ICOSL gene knockout (KO) and wild-type (WT) mice following Chlamydia muridarum lung infection. The results showed that ICOSL KO mice exhibited greater body weight loss, higher pathogen burden, and more severe histopathology in their lung than did WT mice. Cytokine analysis revealed that ICOSL KO mice produced lower levels of Th2 (IL-4 and IL-5) and anti-inflammatory (TGF-\u03b21 and IL-10) cytokines, but higher Th1-related (IFN-\u03b3 and IL-12p40\/IL-23) and proinflammatory (IL-6 and TNF-\u03b1) cytokines. ICOSL KO mice also showed reduced Chlamydia-specific Ab levels in their sera and lung homogenates. In addition, ICOSL KO mice demonstrated significantly lower ICOS expression in T cells and lower Th17 responses than did WT mice. Finally, we showed that ICOS\u2013ICOSL interaction and cell\u2013cell contact are essential for CD4+ T cells to inhibit chlamydial growth in the cultured lung fibroblasts. The data suggest that ICOSL plays a significant role in immunoregulation and protective immunity against Chlamydia infections and that the Th1 skew in cytokine responses per se is not sufficient for effective control of Chlamydia infections.<\/jats:p>","DOI":"10.4049\/jimmunol.0901384","type":"journal-article","created":{"date-parts":[[2010,2,26]],"date-time":"2010-02-26T21:31:19Z","timestamp":1267219879000},"page":"3780-3788","source":"Crossref","is-referenced-by-count":19,"title":["Th1 Cytokine Responses Fail to Effectively Control\n                    <i>Chlamydia<\/i>\n                    Lung Infection in ICOS Ligand Knockout Mice"],"prefix":"10.1093","volume":"184","author":[{"given":"Kamran","family":"Kadkhoda","sequence":"first","affiliation":[{"name":"Laboratory for Infection and Immunity, Department of Medical Microbiology, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}]},{"given":"Shuhe","family":"Wang","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Department of Medical Microbiology, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}]},{"given":"Antony George","family":"Joyee","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Department of Medical Microbiology, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}]},{"given":"Yijun","family":"Fan","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Department of Medical Microbiology, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}]},{"given":"Jie","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Department of Medical Microbiology, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"Laboratory for Infection and Immunity, Department of Medical Microbiology, University of Manitoba , Winnipeg, Manitoba,","place":["Canada"]}]}],"member":"286","published-online":{"date-parts":[[2010,4,1]]},"reference":[{"key":"2026012806331488200_r1","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1016\/S1074-7613(00)80117-X","article-title":"B7h, a novel costimulatory homolog of B7.1 and B7.2, is induced by TNFalpha.","volume":"11","author":"Swallow","year":"1999","journal-title":"Immunity"},{"key":"2026012806331488200_r2","doi-asserted-by":"crossref","first-page":"827","DOI":"10.1038\/45582","article-title":"T-cell co-stimulation through B7RP-1 and ICOS.","volume":"402","author":"Yoshinaga","year":"1999","journal-title":"Nature"},{"key":"2026012806331488200_r3","doi-asserted-by":"crossref","first-page":"1365","DOI":"10.1038\/70932","article-title":"B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion.","volume":"5","author":"Dong","year":"1999","journal-title":"Nat. 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