{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T04:23:56Z","timestamp":1774671836074,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"2_Part_1","license":[{"start":{"date-parts":[[1975,2,1]],"date-time":"1975-02-01T00:00:00Z","timestamp":160444800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1975,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mitogen-induced cytotoxicity was studied by culturing mouse spleen cells with an optimum mitogenic dose of concanavalin A (Con A) for 2 days and, after washing, assessing their ability to lyse tumor targets. We show that rapid lysis occurs only when the correct concentration of an agglutinating mitogen (phytohemagglutinin P (PHA) or Con A) is present in the assay. PHA is more efficient than Con A in revealing the cytotoxic effect. The Con A-induced effector cytotoxic cell is shown to be sensitive to anti-\u03b8 serum and complement. Cytotoxic T cells were also induced by H-2 different allo-immunization. These effector cells specifically lyse targets which bear the immunizing H-2 antigens in the absence of PHA. When PHA is present in the assay, they will also lyse syngeneic tumor targets nonspecifically. Since not all dividing T cells (e.g., T lymphomas, and T blasts induced by M locus different, H-2 similar mixed lymphocyte culture) lyse PHA-P815, we propose that this assay measures only a subset of effector T cells, namely, cytotoxic T cells, regardless of their antigen specificity.<\/jats:p>\n               <jats:p>The tumor cells, P815 and EL4, are sensitive both to antigen-specific T cell-mediated lysis in the absence of PHA and to nonspecific, PHA-revealed lysis. Small lymphocytes, T cell blasts, and B cell blasts are sensitive to lysis by T cells which are directed against H-2 antigens on their surface, but are not very susceptible to nonspecific T cell lysis in the presence of PHA. The reason for this difference in susceptibility of tumor and normal cells to PHA-revealed nonspecific T cell lysis is not known but may have some relevance to in vivo tumor rejection.<\/jats:p>","DOI":"10.4049\/jimmunol.114.2_part_1.559","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T21:00:13Z","timestamp":1672434013000},"page":"559-565","source":"Crossref","is-referenced-by-count":168,"title":["Cytotoxic Effects of Antigen- and Mitogen-Induced T Cells on Various Targets"],"prefix":"10.1093","volume":"114","author":[{"given":"Michael J","family":"Bevan","sequence":"first","affiliation":[{"name":"Salk Institute for Biological Studies , La Jolla, California 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melvin","family":"Cohn","sequence":"additional","affiliation":[{"name":"Salk Institute for Biological Studies , La Jolla, California 92037"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1975,2,1]]},"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/114\/2_Part_1\/559\/62525346\/ji1142p10559.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/114\/2_Part_1\/559\/62525346\/ji1142p10559.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T13:41:38Z","timestamp":1743774098000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/114\/2_Part_1\/559\/8090678"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,2,1]]},"references-count":0,"journal-issue":{"issue":"2_Part_1","published-print":{"date-parts":[[1975,2,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.114.2_part_1.559","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1975,2]]},"published":{"date-parts":[[1975,2,1]]}}}