{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T07:08:34Z","timestamp":1762067314111,"version":"3.40.3"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[1975,11,1]],"date-time":"1975-11-01T00:00:00Z","timestamp":184032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1975,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Subpopulations of human peripheral blood leukocytes were isolated by rosette formation and tested for functional activity. E -rosette-forming cells (E-RFC) and EAC-RFC were separated from non-rosetting cells by sedimentation on Ficoll-Hypaque gradients. The efficiency of the method and the purity of the resulting subpopulations were high. E-RFC responded to PHA Con A, allogeneic leukocytes, and PPD, with higher levels of proliferative reactivity than the unseparated lymphocytes while E-RFC depleted, EAC-RFC, and null cells showed only low levels of reactivity. Reactivity to PWM and tetanus toxoid was also restricted to the E-RFC subpopulation, but was lower than that of unseparated cells. A staphylococcal antigen preparation triggered lymphoproliferative reactivity in the E-RFC, E-RFC deleted, EAC-RFC, and the null cell subpopulations. 51Cr release lymphocyte cytotoxicity against a human lymphoblast target cell line was found in the E-RFC and null cell fractions but was not observed with the EAC-RFC subpopulation.<\/jats:p>","DOI":"10.4049\/jimmunol.115.5.1449","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T21:43:45Z","timestamp":1672436625000},"page":"1449-1455","source":"Crossref","is-referenced-by-count":54,"title":["Functional Activities of Rosette Separated Human Peripheral Blood Leukocytes"],"prefix":"10.1093","volume":"115","author":[{"given":"Jack H","family":"Dean","sequence":"first","affiliation":[{"name":"Department of Immunology, Litton Bionetics, Inc. , Kensington, Maryland 20795"},{"name":"Keesler Medical Center, Keesler Air Force Base , Biloxi, Mississippi 39534"},{"name":"Laboratory of Immunodiagnosis, National Cancer Institute , Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John S","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Immunology, Litton Bionetics, Inc. , Kensington, Maryland 20795"},{"name":"Keesler Medical Center, Keesler Air Force Base , Biloxi, Mississippi 39534"},{"name":"Laboratory of Immunodiagnosis, National Cancer Institute , Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L","family":"McCoy","sequence":"additional","affiliation":[{"name":"Department of Immunology, Litton Bionetics, Inc. , Kensington, Maryland 20795"},{"name":"Keesler Medical Center, Keesler Air Force Base , Biloxi, Mississippi 39534"},{"name":"Laboratory of Immunodiagnosis, National Cancer Institute , Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Connie M","family":"Leonard","sequence":"additional","affiliation":[{"name":"Department of Immunology, Litton Bionetics, Inc. , Kensington, Maryland 20795"},{"name":"Keesler Medical Center, Keesler Air Force Base , Biloxi, Mississippi 39534"},{"name":"Laboratory of Immunodiagnosis, National Cancer Institute , Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Grace B","family":"Cannon","sequence":"additional","affiliation":[{"name":"Department of Immunology, Litton Bionetics, Inc. , Kensington, Maryland 20795"},{"name":"Keesler Medical Center, Keesler Air Force Base , Biloxi, Mississippi 39534"},{"name":"Laboratory of Immunodiagnosis, National Cancer Institute , Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronald B","family":"Herberman","sequence":"additional","affiliation":[{"name":"Department of Immunology, Litton Bionetics, Inc. , Kensington, Maryland 20795"},{"name":"Keesler Medical Center, Keesler Air Force Base , Biloxi, Mississippi 39534"},{"name":"Laboratory of Immunodiagnosis, National Cancer Institute , Bethesda, Maryland 20014"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1975,11,1]]},"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/115\/5\/1449\/62498418\/ji1150051449.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/115\/5\/1449\/62498418\/ji1150051449.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,4,4]],"date-time":"2025-04-04T12:37:51Z","timestamp":1743770271000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/115\/5\/1449\/8089403"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1975,11,1]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1975,11,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.115.5.1449","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"type":"print","value":"0022-1767"},{"type":"electronic","value":"1550-6606"}],"subject":[],"published-other":{"date-parts":[[1975,11]]},"published":{"date-parts":[[1975,11,1]]}}}