{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T07:43:14Z","timestamp":1775029394164,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1979,6,1]],"date-time":"1979-06-01T00:00:00Z","timestamp":297043200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1979,6,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Fusion of cells of the mouse myeloma line, P3\/X63-Ag8 with spleen cells from AKR\/J mice immunized against C3H thymocytes or from (BALB\/c \u00d7 BALB.K)F1 mice immunized against AKR\/J thymocytes gave rise to hybrid cell lines that continuously secrete antibodies specific for the Thy-1.2 and Thy-1.1 antigens, respectively. Monoclonal antibodies from four such cell lines were analyzed in detail. All were 19S IgM, and, in the presence of complement (C), had high lytic titers on T cells of the appropriate antigenicity. Their specificity was shown by lysis of thymocytes from Thy-1 congenic mouse strains, A\/J(Thy-1.2) and A.Thy 1.1. Furthermore, they lyse only 60 to 70% of lymph node cells, suggesting cytotoxicity for mature T cells and not B cells. Treatment of peripheral lymphocyte populations with monoclonal antibody plus C eliminated effector cytotoxic T lymphocytes, their precursors, and the mitogenic response to Con A, but did not affect the response to LPS. Purified, fluorescein-labeled monoclonal anti-Thy-1 antibody could be used to distinguish T and B cells. Purified antibody coupled to Sepharose 6MB was used to separate viable T and B cells. Two independently isolated anti-Thy-1.2 hybridomas are indistinguishable and bind the same determinant whereas a third is unique and may bind a separate site.<\/jats:p>","DOI":"10.4049\/jimmunol.122.6.2491","type":"journal-article","created":{"date-parts":[[2022,12,30]],"date-time":"2022-12-30T17:44:36Z","timestamp":1672422276000},"page":"2491-2497","source":"Crossref","is-referenced-by-count":350,"title":["Properties and Applications of Monoclonal Antibodies Directed against Determinants of the Thy-1 Locus"],"prefix":"10.1093","volume":"122","author":[{"given":"Ann","family":"Marshak-Rothstein","sequence":"first","affiliation":[{"name":"Department of Biology and the Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Fink","sequence":"additional","affiliation":[{"name":"Department of Biology and the Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas","family":"Gridley","sequence":"additional","affiliation":[{"name":"Department of Biology and the Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David H","family":"Raulet","sequence":"additional","affiliation":[{"name":"Department of Biology and the Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J","family":"Bevan","sequence":"additional","affiliation":[{"name":"Department of Biology and the Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malcolm L","family":"Gefter","sequence":"additional","affiliation":[{"name":"Department of Biology and the Center for Cancer Research, Massachusetts Institute of Technology , Cambridge, Massachusetts 02139"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1979,6,1]]},"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/122\/6\/2491\/62439965\/ji1220062491.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/122\/6\/2491\/62439965\/ji1220062491.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,31]],"date-time":"2025-03-31T21:17:44Z","timestamp":1743455864000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/122\/6\/2491\/8084531"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1979,6,1]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1979,6,1]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.122.6.2491","relation":{},"ISSN":["0022-1767","1550-6606"],"issn-type":[{"value":"0022-1767","type":"print"},{"value":"1550-6606","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[1979,6]]},"published":{"date-parts":[[1979,6,1]]}}}