{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T06:22:19Z","timestamp":1779344539284,"version":"3.51.4"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[1992,2,1]],"date-time":"1992-02-01T00:00:00Z","timestamp":696902400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>We have investigated the role of the two AP-1 sites, located at approximately -150 and -180 bp relative to the transcription start site, in induction of the IL-2 promoter through the TCR\/CD3 complex. We show that only the proximal (-150 bp) AP-1 site is functional in vitro, as judged by its ability to bind nuclear proteins from T cells stimulated with Ag or anti-CD3 epsilon. The inducible nuclear proteins binding to this site have the characteristics of AP-1, as judged by their kinetics of induction, the ability to compete and be competed efficiently by a metallothionein AP-1 site oligonucleotide, and their reaction with antibodies to Fos and Jun proteins. Mutations in the proximal AP-1 site greatly diminish or abrogate induction of the IL-2 promoter, indicating that the site is also functional in vivo. Although the distal (-180 bp) AP-1 site is incapable of direct binding to nuclear proteins from activated T cells, a mutation in this site diminishes IL-2 promoter induction, suggesting that this site may also be functional in vivo. Cotransfection of a 5\u2019 IL-2-chloramphenicol acetyltransferase plasmid with c-Fos and\/or c-Jun enhances the induction of IL-2-chloramphenicol acetyltransferase activity, confirming that the IL-2 promoter contains a functional AP-1 site. Both AP-1 sites may be targets for c-Fos action, as inferred from the results of experiments in which c-Fos was cotransfected with internal deletion mutants of the IL-2 promoter lacking either AP-1 site. Northern analysis indicates that mRNAs for at least six members of the Fos\/Jun family (c-fos, fosB, fra-1, c-jun, junB, and junD) are expressed in activated Ar-5 cells; thus the AP-1 sites of the IL-2 promoter may bind different dimeric Fos\/Jun complexes at different times after T cell activation, perhaps mediating both positive and negative regulation of the IL-2 promoter.<\/jats:p>","DOI":"10.4049\/jimmunol.148.4.1240","type":"journal-article","created":{"date-parts":[[2022,12,31]],"date-time":"2022-12-31T09:12:47Z","timestamp":1672477967000},"page":"1240-1250","source":"Crossref","is-referenced-by-count":113,"title":["Analysis of the AP-1 sites in the IL-2 promoter"],"prefix":"10.1093","volume":"148","author":[{"given":"J","family":"Jain","sequence":"first","affiliation":[{"name":"Division of Tumour Virology, Dana-Farber Cancer Institute , Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V E","family":"Valge-Archer","sequence":"additional","affiliation":[{"name":"Division of Tumour Virology, Dana-Farber Cancer Institute , Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Rao","sequence":"additional","affiliation":[{"name":"Division of Tumour Virology, Dana-Farber Cancer Institute , Boston, MA 02115"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1992,2,15]]},"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/148\/4\/1240\/62777771\/1737937.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/148\/4\/1240\/62777771\/1737937.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T01:22:24Z","timestamp":1743297744000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/148\/4\/1240\/8050411"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,2]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[1992,2,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.148.4.1240","relation":{},"ISSN":["1550-6606","0022-1767"],"issn-type":[{"value":"1550-6606","type":"electronic"},{"value":"0022-1767","type":"print"}],"subject":[],"published-other":{"date-parts":[[1992,2]]},"published":{"date-parts":[[1992,2]]}}}