{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T20:44:21Z","timestamp":1771015461941,"version":"3.50.1"},"reference-count":0,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[1992,9,1]],"date-time":"1992-09-01T00:00:00Z","timestamp":715305600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1992,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>We have used APT affinity purification to isolate tyrosine-phosphorylated proteins from MRL lpr\/lpr (lpr) mouse T cells. One such protein is pp81 ezrin, previously identified as a tyrosine-phosphorylated protein in epidermal growth factor-stimulated A431 carcinoma cells. Biochemical analyses in A431 and gastric parietal cells have revealed ezrin to be a cytoskeleton-associated cytosolic protein. In Jurkat T cells, however, using similar methods we have shown ezrin to be a cytosolic protein with no measurable cytoskeletal association. We also observed no increases in ezrin tyrosine phosphorylation in TCR-stimulated Jurkat T cells, unless the cells were pretreated with protein tyrosine phosphatase inhibitors, suggesting that T cell ezrin tyrosine phosphorylation is tightly controlled by protein tyrosine phosphatases. The fraction of tyrosine phosphorylated ezrin in lpr T cells was 5 to 10 times that observed in Jurkat T cells, which along with constitutive TCR-zeta phosphorylation and pp60fyn overexpression, is a feature of the lpr defect.<\/jats:p>","DOI":"10.4049\/jimmunol.149.6.1847","type":"journal-article","created":{"date-parts":[[2022,12,31]],"date-time":"2022-12-31T19:22:15Z","timestamp":1672514535000},"page":"1847-1852","source":"Crossref","is-referenced-by-count":61,"title":["Identification of ezrin as an 81-kDa tyrosine-phosphorylated protein in T cells"],"prefix":"10.1093","volume":"149","author":[{"given":"M","family":"Egerton","sequence":"first","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W H","family":"Burgess","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D","family":"Chen","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B J","family":"Druker","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Bretscher","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L E","family":"Samelson","sequence":"additional","affiliation":[{"name":"Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health , Bethesda, MD 20892"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[1992,9,15]]},"container-title":["The Journal of Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/149\/6\/1847\/62776995\/1381389.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/jimmunol\/article-pdf\/149\/6\/1847\/62776995\/1381389.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,9]],"date-time":"2026-02-09T14:23:43Z","timestamp":1770647023000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jimmunol\/article\/149\/6\/1847\/8054183"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,9]]},"references-count":0,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1992,9,15]]}},"URL":"https:\/\/doi.org\/10.4049\/jimmunol.149.6.1847","relation":{},"ISSN":["1550-6606","0022-1767"],"issn-type":[{"value":"1550-6606","type":"electronic"},{"value":"0022-1767","type":"print"}],"subject":[],"published-other":{"date-parts":[[1992,9]]},"published":{"date-parts":[[1992,9]]}}}